Organization of collagen fibers and tissue hardening: Markers of fibrotic scarring after spinal cord injury in mice revealed by multiphoton-atomic force microscopy imaging

IF 4.2 2区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Clara Manesco MSc , Oscar Saavedra-Villanueva PhD , Marta Martin PhD , Joshua de Lizaraga MSc , Béla Varga PhD , Thierry Cloitre PhD , Yannick Nicolas Gerber PhD , Florence Evelyne Perrin Prof. , Csilla Gergely Prof.
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引用次数: 0

Abstract

Spinal cord injury is a dramatic disease leading to severe motor, sensitive and autonomic impairments. After injury the axonal regeneration is partly inhibited by the glial scar, acting as a physical and chemical barrier. The scarring process involves microglia, astrocytes and extracellular matrix components, such as collagen, constructing the fibrotic component of the scar. To investigate the role of collagen, we used a multimodal label-free imaging approach combining multiphoton and atomic force microscopy. The second harmonic generation signal exhibited by fibrillar collagen enabled to specifically monitor it as a biomarker of the lesion. An increase in collagen density and the formation of more tortuous fibers over time after injury are observed. Nano-mechanical investigations revealed a noticeable hardening of the injured area, correlated with collagen fibers' formation. These observations indicate the concomitance of important structural and mechanical modifications during the fibrotic scar evolution.

Abstract Image

胶原纤维的组织和组织硬化:多光子原子力显微镜成像揭示的小鼠脊髓损伤后纤维瘢痕形成的标志物。
脊髓损伤是一种严重的疾病,会导致严重的运动、敏感和自主神经损伤。损伤后,轴突再生部分受到神经胶质瘢痕的抑制,起到物理和化学屏障的作用。瘢痕形成过程涉及小胶质细胞、星形胶质细胞和细胞外基质成分,如胶原蛋白,构成瘢痕的纤维化成分。为了研究胶原蛋白的作用,我们使用了多光子和原子力显微镜相结合的多模式无标记成像方法。原纤维胶原表现出的二次谐波生成信号能够将其作为病变的生物标志物进行特异性监测。随着时间的推移,观察到损伤后胶原密度的增加和更多弯曲纤维的形成。纳米力学研究显示,损伤区域明显硬化,与胶原纤维的形成有关。这些观察结果表明,在纤维瘢痕的演变过程中,伴随着重要的结构和机械改变。
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来源期刊
CiteScore
11.10
自引率
0.00%
发文量
133
审稿时长
42 days
期刊介绍: The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.
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