The impact of percutaneous epididymal sperm aspiration on sperm quality in mice.

Lisa Windhofer, Auke Boersma, Maik Dahlhoff, Thomas Rülicke, Kerstin E Auer
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Abstract

Abstract: In laboratory mice, sperm quality is usually assessed in spermatozoa collected from the cauda epididymidis of freshly sacrificed males. Percutaneous epididymal sperm aspiration (PESA) is a non-terminal alternative that would allow repeated sperm collection for sperm quality assessment in living males. To test whether PESA is a suitable method to assess sperm quality, we compared sperm traits between samples collected by PESA vs the commonly applied terminal cauda epididymidis dissection. The collected sperm samples were analyzed using computer-assisted sperm analysis and various parameters, including sperm motility, swimming velocity and morphology, were determined. We were able to retrieve motile sperm from all mice using PESA and the terminal cauda epididymidis dissection. Based on computer-assisted sperm analysis, however, sperm motility and swimming velocity were significantly lower after PESA compared to samples obtained by cauda epididymidis dissection. In addition, we found significantly more morphological abnormalities in PESA samples, probably induced as a side effect of the sampling technique. Although sperm samples collected by PESA are successfully used for in vitro fertilization, we cannot recommend PESA as a suitable method to assess sperm quality in mice, since the procedure seems to impair various sperm traits.

Lay summary: In mice, sperm quality is usually assessed in sperm collected from the epididymis (organ where ripe sperm is stored) of euthanized males. However, there is one non-terminal and minimal invasive alternative to collect sperm, called percutaneous epididymal sperm aspiration (PESA), which allows repeated sample collections from the same individual. Given that individual sperm quality is variable and can change according to various factors, PESA could allow to track sperm quality over time and would be highly appreciated in different research fields. Here, we tested the suitability of PESA to determine sperm quality by comparing sperm samples collected by PESA vs the commonly applied terminal epididymis dissection. We used computer-assisted sperm analysis to determine various sperm quality traits. Surprisingly, we found that sperm collected by PESA showed significantly reduced motility, swimming velocity and more morphological abnormalities compared to sperm samples collected by epididymis dissection. Thus, we cannot recommend PESA as a suitable method to determine sperm quality traits as the procedure itself seems to affect collected sperm cells.

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经皮附睾吸精对小鼠精子质量的影响。
摘要:在实验小鼠中,通常用从刚牺牲的雄性附睾尾收集的精子来评估精子质量。经皮附睾精子抽吸(PESA)是一种非终末的选择,允许在活着的男性中重复收集精子以评估精子质量。为了检验PESA是否适合作为精子质量评估的方法,我们比较了PESA与常用的附睾尾端分离法收集的精子的特征。收集的精子样本使用计算机辅助精子分析进行分析,并确定各种参数,包括精子活力,游泳速度和形态。我们使用PESA和附睾末梢解剖从所有小鼠中提取了活动精子。然而,基于计算机辅助精子分析,与通过附睾尾解剖获得的样本相比,PESA后的精子活力和游泳速度明显降低。此外,我们在PESA样本中发现了更多的形态学异常,这可能是采样技术的副作用。尽管PESA收集的精子样本成功地用于体外受精,但我们不能推荐PESA作为评估小鼠精子质量的合适方法,因为该过程似乎损害了精子的各种特征。摘要:在小鼠中,精子质量通常是通过从被安乐死的雄性的附睾(储存成熟精子的器官)收集的精子来评估的。然而,有一种非终末和微创的精子收集方法,称为经皮附睾精子抽吸(PESA),它允许从同一个体重复收集样本。鉴于个体精子质量是可变的,可以根据各种因素而改变,PESA可以随时间跟踪精子质量,并将在不同的研究领域受到高度赞赏。在这里,我们通过比较PESA收集的精子样本与常用的附睾末端解剖来测试PESA测定精子质量的适用性。我们使用计算机辅助精子分析来确定各种精子质量特征。令人惊讶的是,我们发现通过PESA收集的精子与通过附睾解剖收集的精子相比,运动能力、游泳速度明显降低,形态异常更多。因此,我们不能推荐PESA作为确定精子质量特征的合适方法,因为程序本身似乎会影响收集的精子细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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