Plasmid – Chromosome interplay in natural and non-natural hosts: global transcription study of three Bacillus cereus group strains carrying pCER270 plasmid

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology Pub Date : 2023-07-01 DOI:10.1016/j.resmic.2023.104074

Alicia Nevers , Markus Kranzler , Stéphane Perchat , Michel Gohar , Alexei Sorokin , Didier Lereclus , Monika Ehling-Schulz , Vincent Sanchis-Borja

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引用次数: 1

Abstract

The Bacillus cereus group comprises genetically related Gram-positive spore-forming bacteria that colonize a wide range of ecological niches and hosts. Despite their high degree of genome conservation, extrachromosomal genetic material diverges between these species. The discriminating properties of the B. cereus group strains are mainly due to plasmid-borne toxins, reflecting the importance of horizontal gene transfers in bacterial evolution and species definition. To investigate how a newly acquired megaplasmid can impact the transcriptome of its host, we transferred the pCER270 from the emetic B. cereus strains to phylogenetically distant B. cereus group strains. RNA-sequencing experiments allowed us to determine the transcriptional influence of the plasmid on host gene expression and the impact of the host genomic background on the pCER270 gene expression. Our results show a transcriptional cross-regulation between the megaplasmid and the host genome. pCER270 impacted carbohydrate metabolism and sporulation genes expression, with a higher effect in the natural host of the plasmid, suggesting a role of the plasmid in the adaptation of the carrying strain to its environment. In addition, the host genomes also modulated the expression of pCER270 genes. Altogether, these results provide an example of the involvement of megaplasmids in the emergence of new pathogenic strains.

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质粒-染色体在自然和非自然宿主中的相互作用:三个携带pCER270质粒的蜡样芽孢杆菌群菌株的全球转录研究

蜡样芽孢杆菌群包括在广泛的生态位和宿主中定植的遗传相关的革兰氏阳性孢子形成细菌。尽管它们具有高度的基因组保守性，但染色体外遗传物质在这些物种之间存在差异。蜡状芽孢杆菌群菌株的鉴别特性主要是由于质粒携带的毒素，反映了水平基因转移在细菌进化和物种定义中的重要性。为了研究新获得的大质粒如何影响其宿主的转录组，我们将pCER270从呕吐的蜡状芽孢杆菌菌株转移到系统发育上遥远的蜡状分枝杆菌群菌株。RNA测序实验使我们能够确定质粒对宿主基因表达的转录影响以及宿主基因组背景对pCER270基因表达的影响。我们的结果显示了大质粒和宿主基因组之间的转录交叉调节。pCER270影响碳水化合物代谢和孢子形成基因的表达，在质粒的天然宿主中具有更高的作用，表明质粒在携带菌株适应其环境中的作用。此外，宿主基因组还调节pCER270基因的表达。总之，这些结果提供了一个大质粒参与新致病菌株出现的例子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.