Efficacy of soy protein concentrate replacing animal protein supplements in mucosa-associated microbiota, intestinal health, and growth performance of nursery pigs

IF 6.3
Zixiao Deng, Marcos Elias Duarte, Sung Woo Kim
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引用次数: 0

Abstract

This study investigated the effects of using soy protein concentrate (SPC) to replace animal protein supplements on mucosa-associated microbiota, intestinal health, and growth performance of nursery pigs. Fifty-six newly weaned pigs (BW = 6.4 ± 0.6 kg) were allotted to 5 treatments in a randomized complete block design. Pigs were fed for 35 d in 3 phases (P; 1, 2, 3) for 10, 12, 13 d, respectively. Dietary treatments were: (1) basal diet with fish meal (P1: 4%, P2: 2%, and P3: 1%), poultry meal (P1: 10%, P2: 8%, and P3: 4%), and blood plasma (P1: 4%, P2: 2%, and P3: 1%), where SPC replacing none (NC); (2) basal diet with SPC replacing fish meal (RFM); (3) basal diet with SPC replacing poultry meal (RPM); (4) basal diet with SPC replacing blood plasma (RBP); and (5) basal diet with SPC replacing all animal protein supplements (PC). Growth performance was recorded for each phase. Pigs were euthanized on d 35 to collect jejunal mucosa and tissue to evaluate intestinal health and microbiota, and ileal digesta to measure apparent ileal digestibility (AID) of nutrients. Data were analyzed using the MIXED procedure of SAS. Overall, RFM, RPM, and RBP did not affect growth performance, whereas PC decreased (P < 0.05) ADG and ADFI. The RPM increased (P < 0.05) Prevotella stercorea and decreased (P < 0.05) Helicobacter rappini. The PC decreased (P < 0.05) H. rappini, whilst increasing (P < 0.05) Prevotella copri, Propionibacterium acnes, and Pelomonas aquatica. The RFM tended to increase (P = 0.096) immunoglobulin A in the jejunum. The PC tended to decrease (P = 0.078) jejunal crypt cell proliferation. There were no differences in the villus height, AID of nutrients, intestinal inflammation, and intestinal oxidative stress among treatments. In conclusion, SPC can replace fish meal, poultry meal, or blood plasma individually without affecting growth performance and intestinal health, and AID of nutrients of nursery pigs. Particularly SPC replacing poultry meal benefitted intestinal health by reducing H. rappini and increasing P. stercorea. However, SPC replacing all three animal protein supplements reduced growth of nursery pigs mainly by reducing feed intake.

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大豆浓缩蛋白替代动物蛋白补充剂对仔猪粘膜相关微生物群、肠道健康和生长性能的影响
本研究调查了使用大豆浓缩蛋白(SPC)代替动物蛋白补充剂对仔猪粘膜相关微生物群、肠道健康和生长性能的影响。56头新断奶的猪(体重=6.4±0.6kg)被分配到5个处理中,采用随机完全区组设计。猪分3个阶段饲养35天(P;1、2、3),分别饲养10、12、13天。日粮处理为:(1)基础日粮,鱼粉(P1:4%、P2:2%和P3:1%)、家禽粉(P1:10%、P2:8%和P3:4%)和血浆(P1:4%、P2:2%和P3:1%),其中SPC取代无(NC);(2) SPC替代鱼粉(RFM)的基础日粮;(3) 用SPC代替家禽粉的基础日粮(RPM);(4) SPC替代血浆(RBP)的基础日粮;和(5)SPC取代所有动物蛋白补充剂(PC)的基础饮食。记录每个阶段的增长表现。在第35天对猪实施安乐死,收集空肠粘膜和组织以评估肠道健康和微生物群,并收集回肠消化物以测量营养物质的表观回肠消化率(AID)。使用SAS的MIXED程序对数据进行分析。总体而言,RFM、RPM和RBP不影响生长性能,而PC降低了ADG和ADFI(P<;0.05)。RPM增加(P<;0.05)斯特科雷沃氏菌,并降低(P<)幽门螺杆菌。PC降低了(P<;0.05)H.rappini,同时增加了(P>;0.05)粪普雷沃氏菌、痤疮丙酸杆菌和水性Pelomonas aquatica。RFM倾向于增加(P=0.096)空肠中的免疫球蛋白A。PC有减少空肠隐窝细胞增殖的趋势(P=0.078)。不同治疗组在绒毛高度、营养物质AID、肠道炎症和肠道氧化应激方面没有差异。总之,SPC可以单独替代鱼粉、家禽粉或血浆,而不会影响生长性能和肠道健康,也不会影响育肥猪营养物质的AID。特别是SPC替代了家禽粉,通过减少H.rappini和增加P.stercorea有益于肠道健康。然而,SPC替代了所有三种动物蛋白补充剂,主要通过减少饲料摄入量来减少育肥猪的生长。
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来源期刊
Animal Nutrition
Animal Nutrition Animal Science and Zoology
CiteScore
9.70
自引率
0.00%
发文量
542
审稿时长
65 days
期刊介绍: Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.
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