Magnesium suppresses in vivo oxidative stress and ex vivo DNA damage induced by protracted ACTH treatment in rats.

IF 1.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Vedrana Đurić, Jelena Petrović, Dušanka Stanić, Ana Ivanović, Jelena Kotur-Stevuljević, Vesna Pešić
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引用次数: 0

Abstract

Oxidative stress, arising from disrupted balance between reactive oxygen/nitrogen species (ROS/RNS) and antioxidant defences, has been implicated in the pathogenesis of stress-related disorders. There is a growing body of evidence that supports the relationship between the activity of the hypothalamic-pituitary-adrenal (HPA) stress system, oxidative stress and magnesium (Mg) homeostasis. The present study aimed to explore the gap in our current understanding of antigenotoxic and protective effects of Mg supplementation against excessive ROS production in male rats during chronic treatment with adrenocorticotropic hormone (ACTH). Our findings show that exposure to exogenous ACTH (10 μg/day, s.c., for 21 days), as one of the key mediators of the HPA axis and stress response, produced an increase in superoxide anion levels and a decrease in superoxide dismutase activity in plasma. We observed that Mg supplementation, starting seven days prior to ACTH treatment and lasting 28 days (300 mg/L of drinking water, per os), abolished these effects in experimental animals. Moreover, our study reveals that ACTH increased the susceptibility of peripheral blood lymphocytes to ex vivo H2O2-induced total and high-level oxidative DNA damage, while Mg completely reversed these effects. Collectively, these results highlight the promising role of Mg in stress-related conditions accompanied by increased oxidative stress in animals and support further investigation using human dietary trials.

镁对长时间ACTH诱导的大鼠体内氧化应激和体外DNA损伤有抑制作用。
氧化应激是由活性氧/氮(ROS/RNS)和抗氧化防御之间的平衡被破坏引起的,与应激相关疾病的发病机制有关。越来越多的证据支持下丘脑-垂体-肾上腺(HPA)应激系统、氧化应激和镁(Mg)稳态之间的关系。本研究旨在探讨我们目前对慢性促肾上腺皮质激素(ACTH)治疗期间补充Mg对雄性大鼠过量ROS产生的抗原毒性和保护作用的理解差距。我们的研究结果表明,外源性ACTH (10 μg/d, s.c,持续21天)作为HPA轴和应激反应的关键介质之一,会导致血浆超氧化物阴离子水平升高,超氧化物歧化酶活性降低。我们观察到,在ACTH治疗前7天开始补充Mg,持续28天(每天300 Mg /L饮用水),在实验动物中消除了这些影响。此外,我们的研究表明,ACTH增加了外周血淋巴细胞对体外h2o2诱导的总氧化性和高水平氧化性DNA损伤的敏感性,而Mg完全逆转了这些作用。总的来说,这些结果强调了Mg在动物氧化应激增加的应激相关条件下的有希望的作用,并支持通过人类饮食试验进行进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Magnesium research
Magnesium research 医学-内分泌学与代谢
CiteScore
3.50
自引率
9.40%
发文量
6
审稿时长
>12 weeks
期刊介绍: Magnesium Research, the official journal of the international Society for the Development of Research on Magnesium (SDRM), has been the benchmark journal on the use of magnesium in biomedicine for more than 30 years. This quarterly publication provides regular updates on multinational and multidisciplinary research into magnesium, bringing together original experimental and clinical articles, correspondence, Letters to the Editor, comments on latest news, general features, summaries of relevant articles from other journals, and reports and statements from national and international conferences and symposiums. Indexed in the leading medical databases, Magnesium Research is an essential journal for specialists and general practitioners, for basic and clinical researchers, for practising doctors and academics.
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