Polysorbate 80 surface modified PLGA nanoparticles: an in-vitro evaluation of cellular uptake and cytotoxicity on neuro-2a cells.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2023-11-01 Epub Date: 2023-08-23 DOI:10.1080/02652048.2023.2244095

Surbhi Sharma, Shweta Dang

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引用次数: 1

Abstract

Aim: Present study focuses on the development of P80 coated PLGA Nanoparticles loaded with drugs, paroxetine (P80-Par-PLGA-NPs) and clonidine (P80-CLD-PLGA-NPs) for in-vitro evaluation of Cellular Uptake & Cytotoxicity on Neuro-2a cells.

Method: P80-Par-PLGA-NPs and P80-CLD-PLGA-NPs were developed and characterised for zeta size, potential, PDI, EE%, DL%, TEM, SEM, FTIR, DSC, in-vitro release, cytotoxicity, histopathological and cell uptake studies using rhodamine loaded P80-NPs.

Result: Mean particle diameter of P80-Par-PLGA-NPs and P80-CLD-PLGA-NPs was 204; 182.7 nm, ZP of -21.8; -18.72 mV and 0.275; 0.341 PDI, respectively. TEM and SEM images revealed homogenous surface morphology. In-vitro drug release showed sustained and complete release in 72 h. Cell viability (>90%) at C_max and no cytotoxicity in histopathology was observed. Significant higher uptake (96.9%) of P80-modified-NPS was observed as compared to unmodified-NPs (81%) (p < 0.05).

Conclusion: The finding clearly indicated a higher cell uptake of drugs via surface modified P80-coated PLGA-NPs as compared to unmodified particles.

查看原文本刊更多论文

聚山梨醇酯80表面修饰的PLGA纳米颗粒：细胞摄取和对神经-2a细胞的细胞毒性的体外评估。

目的：本研究的重点是开发负载药物、帕罗西汀（P80-Par-PLGA NPs）和可乐定（P80-CLD-PLGA NPs）的P80包被PLGA纳米颗粒，用于体外评估Neuro-2a细胞的细胞摄取和细胞毒性。方法：使用罗丹明负载的P80 NPs开发并表征P80-Par-PLGA NPs和P80-CLD-PLGA NPs的ζ大小、电位、PDI、EE%、DL%、TEM、SEM、FTIR、DSC、体外释放、细胞毒性、组织病理学和细胞摄取研究。结果：P80-Par-PLGA NPs和P80-CLD-PLGA NPs的平均粒径为204；182.7 nm，ZP为-21.8-18.72 mV和0.275；分别为0.341 PDI。TEM和SEM图像显示了均匀的表面形态。体外药物释放显示持续和完全释放72 h.Cmax时的细胞活力（>90%），组织病理学中未观察到细胞毒性。与未修饰的NPS（81%）相比，观察到P80修饰的NPS的摄取显著更高（96.9%）（p 结论：与未修饰的颗粒相比，该发现清楚地表明，通过表面修饰的P80包被的PLGA NPs，细胞对药物的摄取更高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.