Dysbiosis of gut microbiota during fecal stream diversion in patients with colorectal cancer.

IF 4.3 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Gut Pathogens Pub Date : 2023-08-18 DOI:10.1186/s13099-023-00566-9

Soo Young Lee, Hyeung-Min Park, Chang Hyun Kim, Hyeong Rok Kim

{"title":"Dysbiosis of gut microbiota during fecal stream diversion in patients with colorectal cancer.","authors":"Soo Young Lee, Hyeung-Min Park, Chang Hyun Kim, Hyeong Rok Kim","doi":"10.1186/s13099-023-00566-9","DOIUrl":null,"url":null,"abstract":"Background: The effect of fecal stream diversion on the gut microbiota is still uncertain. The present study was designed to assess the effect of fecal stream diversion on the composition of the gut microbiota in patients with colorectal cancer. We included patients undergoing left-sided colorectal cancer surgery with (ileostomy group) or without (control group) diverting ileostomy. Fecal samples were collected from 10 patients in each group before surgery (t1) and after ileostomy repair in the ileostomy group and 6-12 months after the initial surgery in the control group (t2). The fecal microbiota was assessed using 16S rRNA sequencing, and changes in the composition of the fecal microbiota were compared between the two groups.Results: Alpha diversity analysis revealed that the complexity of fecal microbiota decreased between t1 and t2 only in the ileostomy group. Beta diversity analysis also showed dissimilarity between t1 and t2 only in the ileostomy group. The composition of the microbiota was similar between the two groups at t1. However, at t2, the ileostomy group had lower proportion of beneficial bacteria (Lachnospiraceae, 3.8% vs. 29.9%, p < 0.001; Ruminococcaceae, 0.6% vs. 18.4%, p < 0.001; Blautia, 0.1% vs. 9.1%, p < 0.001; Faecalibacterium, 0.2% vs. 7.5%, p < 0.001) and a higher proportion of harmful bacteria (Proteobacteria, 17.9% vs. 5.1%, p = 0.006; Clostridium, 16.2% vs. 1.1%, p = 0.013; Streptococcus, 17.7% vs. 1.6%, p = 0.002) than the control group.Conclusions: Fecal stream diversion was closely associated with less diversity and dysbiosis of the gut microbiota.","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":"15 1","pages":"40"},"PeriodicalIF":4.3000,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439566/pdf/","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gut Pathogens","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s13099-023-00566-9","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}

引用次数: 1

Abstract

Background: The effect of fecal stream diversion on the gut microbiota is still uncertain. The present study was designed to assess the effect of fecal stream diversion on the composition of the gut microbiota in patients with colorectal cancer. We included patients undergoing left-sided colorectal cancer surgery with (ileostomy group) or without (control group) diverting ileostomy. Fecal samples were collected from 10 patients in each group before surgery (t₁) and after ileostomy repair in the ileostomy group and 6-12 months after the initial surgery in the control group (t₂). The fecal microbiota was assessed using 16S rRNA sequencing, and changes in the composition of the fecal microbiota were compared between the two groups.

Results: Alpha diversity analysis revealed that the complexity of fecal microbiota decreased between t₁ and t₂ only in the ileostomy group. Beta diversity analysis also showed dissimilarity between t₁ and t₂ only in the ileostomy group. The composition of the microbiota was similar between the two groups at t₁. However, at t₂, the ileostomy group had lower proportion of beneficial bacteria (Lachnospiraceae, 3.8% vs. 29.9%, p < 0.001; Ruminococcaceae, 0.6% vs. 18.4%, p < 0.001; Blautia, 0.1% vs. 9.1%, p < 0.001; Faecalibacterium, 0.2% vs. 7.5%, p < 0.001) and a higher proportion of harmful bacteria (Proteobacteria, 17.9% vs. 5.1%, p = 0.006; Clostridium, 16.2% vs. 1.1%, p = 0.013; Streptococcus, 17.7% vs. 1.6%, p = 0.002) than the control group.

Conclusions: Fecal stream diversion was closely associated with less diversity and dysbiosis of the gut microbiota.

查看原文本刊更多论文

结直肠癌患者粪流转移过程中肠道菌群的生态失调。

背景:粪流改道对肠道菌群的影响尚不确定。本研究旨在评估粪便分流对结直肠癌患者肠道菌群组成的影响。我们纳入了接受左侧结肠直肠癌手术的患者(回肠造口组)或未(对照组)转移回肠造口。每组10例患者于手术前(t1)、造口组于造口修复后、对照组于手术后6-12个月(t2)采集粪便样本。采用16S rRNA测序评估粪便微生物群，比较两组粪便微生物群组成的变化。结果:α多样性分析显示，只有回肠造口组在t1和t2之间粪便微生物群的复杂性降低。β多样性分析也显示仅回肠造口组t1与t2之间存在差异。t1时两组菌群组成相似。然而，在t2时，回肠造口组的有益菌比例较低(毛缕菌科，3.8%比29.9%)。结论:粪便流转移与肠道微生物群多样性减少和生态失调密切相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY

CiteScore

7.70

自引率

2.40%

发文量

期刊介绍： Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).