Establishment risk of invasive golden mussel in a water diversion project: An assessment framework

Abstract

Inter-basin water diversion projects have led to accelerated colonization of aquatic organisms, including the freshwater golden mussel (Limnoperna fortunei), exacerbating global biofouling concerns. While the influence of environmental factors on the mussel's invasion and biofouling impact has been studied, quantitative correlations and underlying mechanisms remain unclear, particularly in large-scale inter-basin water diversion projects with diverse hydrodynamic and environmental conditions. Here, we examine the comprehensive impact of environmental variables on the establishment risk of the golden mussel in China's 1432-km-long Middle Route of the South-to-North Water Diversion Project. Logistic regression and multiclass classification models were used to investigate the environmental influence on the occurrence probability and reproductive density of the golden mussel. Total nitrogen, ammonia nitrogen, water temperature, pH, and velocity were identified as crucial environmental variables affecting the biofouling risk in the project. Logistic regression analysis revealed a negative correlation between the occurrence probability of all larval stages and levels of total nitrogen and ammonia nitrogen. The multiclass classification model showed that elevated levels of total nitrogen hindered mussel reproduction, while optimal water temperature enhanced their reproductive capacity. Appropriate velocity and pH levels were crucial in maintaining moderate larval density. This research presents a quantitative analytical framework for assessing establishment risks associated with invasive mussels, and the framework is expected to enhance invasion management and mitigate biofouling issues in water diversion projects worldwide.