Association of epidemiological and clinical features with PCR cycle threshold values of SARS-CoV-2 infection: a cross-sectional study.

IF 4.9 4区 医学 Q1 PARASITOLOGY
Pathogens and Global Health Pub Date : 2023-07-01 Epub Date: 2022-12-15 DOI:10.1080/20477724.2022.2158003
Marlies Heudobler, Hansjörg Baurecht, Helen Schmied, Daniel Heudobler, Carmen Jochem, Anja M Sedlmeier, Andrea Weber, Stilla Bauernfeind, Michael Leitzmann, Bernd Salzberger, Benedikt M J Lampl
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引用次数: 1

Abstract

The cycle threshold (Ct) in quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) is inversely correlated to the amount of viral nucleic acid or viral load and can be regarded as an indicator of infectivity. We examined the association of socio-demographic and clinical characteristics of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) polymerase chain reaction (PCR) positive cases with PCR cycle threshold (Ct) values at the time of diagnosis. SARS-CoV-2 cases reported between 12 October 2020 and 24 January 2021 in Regensburg were analyzed employing bivariate and multivariable methods. We included 3,029 SARS-CoV-2 cases (31% asymptomatic at diagnosis) and analyzed the association of case characteristics with Ct values in 2,606 cases. Among symptomatic patients, cough (38.0%), rhinitis (32.4%), headache (32.0), and fever/chills (29.9%) were the most frequent complaints. Ct values ≤20 were more frequent in symptomatic cases (20.9% vs. 11.3%), whereas Ct values >30 were more common in asymptomatic cases (32.6% vs. 18.0%). Ct values >20 and ≤30 were most common in symptomatic and asymptomatic cases (48.0% vs 40.7%). We observed lower median Ct values of E and N gene in symptomatic cases. In a random forest model, the total number of symptoms, respiratory symptoms, and age were most strongly associated with low Ct values. In conclusion, certain symptoms and age were associated with lower Ct values. Ct values can be used as a pragmatic approach in estimating infectivity at the first notification of a case and, thus, in guiding containment measures.

流行病学和临床特征与 SARS-CoV-2 感染 PCR 周期阈值的关系:一项横断面研究。
定量实时反转录酶聚合酶链反应(qRT-PCR)中的循环阈值(Ct)与病毒核酸或病毒载量成反比,可被视为传染性指标。我们研究了严重急性呼吸系统综合征冠状病毒 2 型(SARS-CoV-2)聚合酶链反应(PCR)阳性病例的社会人口学和临床特征与诊断时 PCR 周期阈值(Ct)的关联。我们采用双变量和多变量方法对雷根斯堡 2020 年 10 月 12 日至 2021 年 1 月 24 日期间报告的 SARS-CoV-2 病例进行了分析。我们纳入了 3,029 例 SARS-CoV-2 病例(31% 在确诊时无症状),并分析了 2,606 例病例的特征与 Ct 值的关系。在有症状的患者中,咳嗽(38.0%)、鼻炎(32.4%)、头痛(32.0)和发热/发冷(29.9%)是最常见的主诉。Ct值≤20在无症状病例中更为常见(20.9%对11.3%),而Ct值>30在无症状病例中更为常见(32.6%对18.0%)。Ct值>20和≤30在有症状和无症状病例中最为常见(48.0% vs 40.7%)。在无症状病例中,我们观察到 E 和 N 基因的中位 Ct 值较低。在随机森林模型中,症状总数、呼吸道症状和年龄与低 Ct 值的关系最为密切。总之,某些症状和年龄与较低的 Ct 值有关。Ct 值可作为一种实用方法,用于估计首次发现病例时的感染率,从而指导遏制措施。
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来源期刊
Pathogens and Global Health
Pathogens and Global Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-PARASITOLOGY
CiteScore
6.00
自引率
0.00%
发文量
60
审稿时长
6-12 weeks
期刊介绍: Pathogens and Global Health is a journal of infectious disease and public health that focuses on the translation of molecular, immunological, genomics and epidemiological knowledge into control measures for global health threat. The journal publishes original innovative research papers, reviews articles and interviews policy makers and opinion leaders on health subjects of international relevance. It provides a forum for scientific, ethical and political discussion of new innovative solutions for controlling and eradicating infectious diseases, with particular emphasis on those diseases affecting the poorest regions of the world.
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