Complete genome sequence of Streptomyces sp. HNA39, a new cyclizidine producer isolated from a South China Sea sediment

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-08-01 DOI:10.1016/j.margen.2023.101033

Suzhen Li , Yong-Jun Jiang , Zhongjun Ma , Nan Wang

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引用次数: 1

Abstract

Streptomyces sp. HNA39 is a promising candidate for the production of antineoplastic metabolites screened from a collection of 448 actinomycetes derived from coastal sediments. The complete genome sequence of HNA39 comprises a 7,351,753-bp linear chromosome with a GC content of 71.94%. Whole genome analysis reveals the presence of 29 putative biosynthetic gene clusters (BGCs) encoding secondary metabolites. Among them, a type I PKS BGC shows an 82% similarity with the cyclizidine (CLD) BGC identified from Streptomyces NCIB 11649. LC-MS profiles further supported the production of new CLD congeners. Bafilomycins were also found produced in abundance, corresponding to another type I PKS BGC highly homologous to that of bafilomycin B1 from S. lohii. CLDs are indolizidine alkaloids consisting a fused five- and six-membered ring system with an intriguing cyclopropane terminal linked by a trans-dienic chain. The cyclization mechanism of the cylopropyl ring, one of its pharmacophores, is still unknown. Genome sequencing of the new CLD producer and subsequent comparative analysis of their gene clusters would further our understanding of the chemistry behind cyclopropane formation.

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从南海沉积物中分离到的一种新的环苯胺生成菌链霉菌HNA39的全基因组序列

链霉菌HNA39是从448种海岸沉积物放线菌中筛选出的一种很有前途的抗肿瘤代谢产物的候选菌株。HNA39的全基因组序列包括一条7351753bp的线性染色体，GC含量为71.94%。全基因组分析显示，存在29个编码次级代谢产物的推定生物合成基因簇（BGCs）。其中，I型PKS BGC与从链霉菌NCIB 11649中鉴定的环叠啶（CLD）BGC显示出82%的相似性。LC-MS图谱进一步支持了新的CLD同源物的生产。巴菲洛霉素也被发现大量产生，对应于另一种与来自S.lohii的巴菲洛菌素B1高度同源的I型PKS BGC。CLDs是一种中氮茚生物碱，由一个稠合的五元和六元环系统组成，该系统具有一个由反式二烯链连接的有趣的环丙烷末端。其药效团之一的酰基丙基环的环化机制尚不清楚。新的CLD生产商的基因组测序和随后对其基因簇的比较分析将进一步加深我们对环丙烷形成背后化学的理解。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.