The LRG-TGF-β-Alk-1/TGFßRII-Smads as Predictive Biomarkers of Chronic Hydrocephalus after Aneurysmal Subarachnoid Hemorrhage.

IF 0.9 4区医学 Q4 CLINICAL NEUROLOGY

Journal of neurological surgery. Part A, Central European neurosurgery Pub Date : 2024-09-01 Epub Date: 2023-08-21 DOI:10.1055/s-0043-1771277

Dongying Ma, Luyao Ma, Yongqiang Zhao, Yongli Li, Wei Ye, Xianfeng Li

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引用次数: 0

Abstract

Background: Chronic hydrocephalus is a common complication of aneurysmal subarachnoid hemorrhage (aSAH); however, the risk factors and the mechanisms underlying its occurrence have yet to be fully elucidated. The purpose of this study was to identify biomarkers that could be used to predict chronic hydrocephalus after aSAH and to investigate the relationships.

Methods: We analyzed cerebrospinal fluid (CSF) samples from 19 patients with chronic hydrocephalus after aSAH and 44 controls without hydrocephalus after aSAH. Enzyme-linked immunosorbent assay was used to determine the levels of leucine-rich alpha-2-glycoprotein (LRG), transforming growth factor-β (TGF-β), Smad1, Smad4, Smad5, Smad8, activin receptor-like kinase 1 (Alk-1), activin receptor-like kinase 5 (Alk-5), P38, and TGF-β type II receptor (TGFßRII) in CSF samples.

Results: In the CSF of patients with chronic hydrocephalus after aSAH, the levels of LRG, TGF-β, Alk-1, Smad5, and TGFßRII were significantly increased (p < 0.05) and the levels of Smad1, Smad4, and Smad8 were significantly decreased (p < 0.05). There were no significant differences between the two groups concerning the levels of P38 and Alk-5 (p > 0.05). The analysis also identified significant correlations between specific biomarkers: LRG and Smad1, LRG and Smad5, TGF-β and Alk-1, and Alk-1 and Smad4 (p < 0.05); the Pearson's correlation coefficients for these relationships were -0.341, 0.257, 0.256, and -0.424, respectively.

Conclusion: The levels of LRG, TGF-β, Alk-1, TGFßRII, Smad1/5/8, and Smad4 in the CSF are potentially helpful as predictive biomarkers of chronic hydrocephalus after aSAH. Moreover, the LRG-TGF-β-Alk-1/TGFßRII-Smad1/5/8-Smad4 signaling pathway is highly likely to be involved in the pathogenic process of chronic hydrocephalus after aSAH.

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LRG-TGF-β-Alk-1/TGFßRII-Smads作为动脉瘤性蛛网膜下腔出血后慢性脑积水的预测性生物标志物。

背景：慢性脑积水是动脉瘤性蛛网膜下腔出血（aSAH）的常见并发症；然而，其发生的风险因素和机制尚未完全阐明。本研究的目的是确定可用于预测动脉瘤性蛛网膜下腔出血后慢性脑积水的生物标志物，并研究其中的关系：方法：我们分析了来自 19 名急性脑积水后慢性脑积水患者和 44 名急性脑积水后无脑积水对照者的脑脊液（CSF）样本。采用酶联免疫吸附法测定脑脊液样本中富含亮氨酸的α-2-糖蛋白（LRG）、转化生长因子-β（TGF-β）、Smad1、Smad4、Smad5、Smad8、激活素受体样激酶 1（Alk-1）、激活素受体样激酶 5（Alk-5）、P38 和 TGF-β II 型受体（TGFßRII）的水平：结果：在SAH 后慢性脑积水患者的脑脊液中，LRG、TGF-β、Alk-1、Smad5 和 TGFßRII 的水平显著升高（p p > 0.05）。分析还确定了特定生物标志物之间的重要相关性：LRG 与 Smad1、LRG 与 Smad5、TGF-β 与 Alk-1、Alk-1 与 Smad4（p 结论：LRG、TGF-β、Smad5 和 TGFßRII 的水平均明显升高（p p > 0.05）：脑脊液中的 LRG、TGF-β、Alk-1、TGFßRII、Smad1/5/8 和 Smad4 水平可作为预测SAH 后慢性脑积水的生物标志物。此外，LRG-TGF-β-Alk-1/TGFßRII-Smad1/5/8-Smad4 信号通路极有可能参与了 aSAH 后慢性脑积水的致病过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of neurological surgery. Part A, Central European neurosurgery CLINICAL NEUROLOGY-SURGERY

CiteScore

2.30

自引率

0.00%

发文量

期刊介绍： The Journal of Neurological Surgery Part A: Central European Neurosurgery (JNLS A) is a major publication from the world''s leading publisher in neurosurgery. JNLS A currently serves as the official organ of several national neurosurgery societies. JNLS A is a peer-reviewed journal publishing original research, review articles, and technical notes covering all aspects of neurological surgery. The focus of JNLS A includes microsurgery as well as the latest minimally invasive techniques, such as stereotactic-guided surgery, endoscopy, and endovascular procedures. JNLS A covers purely neurosurgical topics.