Polymerase chain reaction-based typing methods and protein profiling analysis of Acinetobacter baumannii isolated from environmental and clinical sources from South India.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-11-01 Epub Date: 2023-06-26 DOI:10.1139/cjm-2023-0010

Srajana Nayak, D'Almeida Aanice, Dsouza Andria, Archana Pai, Biswajit Maiti

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Abstract

Acinetobacter baumannii is an opportunistic pathogen known for causing hospital-acquired infections. The natural habitat includes soil, water, sewage, and drains, but it is also detected in infected individuals' blood, pus, and respiratory pathways. Due to its resilient nature, it is known to be a causative agent for outbreaks. Therefore, it is crucial to understand the genetic similarity between clinical and environmental isolates. The study aimed to find the genetic relationships between clinical and environmental isolates using PCR-based typing methods such as enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR), random amplified polymorphic DNA (RAPD), and repetitive extragenic palindromic sequence-based PCR (Rep-PCR). Additionally, outer membrane protein (OMP) and whole cell protein (WCP) profiles were also used. The PCR-based methods, ERIC-PCR and Rep-PCR, showed decreased genetic similarity between clinical and environmental isolates (66% and 58%, respectively). However, RAPD showed relatively higher genetic similarity (91%). The OMP and WCP profiles showed varied banding patterns between the clinical and environmental isolates in the 29-43 kDa region. The PCR-based methods proved to be a reliable and reproducible technique. The OMP and WCP profiles, though not as discriminatory as the molecular typing methods, could help identify the most and least commonly occurring protein bands and thus help in typing clinical and environmental A. baumannii isolates.

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基于聚合酶链式反应的分型方法和从南印度环境和临床来源分离的鲍曼不动杆菌的蛋白质谱分析。

鲍曼不动杆菌是一种机会性病原体，以引起医院获得性感染而闻名。自然栖息地包括土壤、水、污水和下水道，但在感染者的血液、脓液和呼吸道中也能检测到。由于其弹性，它被认为是疫情爆发的病原体。因此，了解临床分离株和环境分离株之间的遗传相似性至关重要。本研究旨在使用基于PCR的分型方法，如肠道细菌重复基因间一致序列PCR（ERIC-PCR）、随机扩增多态性DNA（RAPD）和基于重复基因外回文序列的PCR（Rep-PCR），来寻找临床和环境分离株之间的遗传关系。此外，还使用了外膜蛋白（OMP）和全细胞蛋白（WCP）图谱。基于PCR的ERIC-PCR和Rep-PCR方法显示，临床和环境分离株之间的遗传相似性降低（分别为66%和58%）。然而，RAPD表现出相对较高的遗传相似性（91%）。在29-43 kDa区域。基于PCR的方法被证明是一种可靠且可重复的技术。OMP和WCP图谱虽然不像分子分型方法那样具有歧视性，但有助于识别最常见和最不常见的蛋白带，从而有助于对临床和环境鲍曼不动杆菌分离株进行分型。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.