Decellularized tendon scaffolds loaded with collagen targeted extracellular vesicles from tendon-derived stem cells facilitate tendon regeneration

IF 10.5 1区医学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Journal of Controlled Release Pub Date : 2023-08-01 DOI:10.1016/j.jconrel.2023.07.032

Jing Cui , Yan-Jing Zhang , Xuan Li, Jia-Jiao Luo, Lei-Lei Zhao, Xin-Yue Xie, Wei Ding, Jing-Cong Luo, Ting-Wu Qin

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Abstract

Stem cell-based treatment of tendon injuries remains to have some inherent issues. Extracellular vesicles derived from stem cells have shown promising achievements in tendon regeneration, though their retention in vivo is low. This study reports on the use of a collagen binding domain (CBD) to bind extracellular vesicles, obtained from tendon-derived stem cells (TDSCs), to collagen. CBD-extracellular vesicles (CBD-EVs) were coupled to decellularized bovine tendon sheets (DBTS) to fabricate a bio-functionalized scaffold (CBD-EVs-DBTS). Our results show that thus obtained bio-functionalized scaffolds facilitate the proliferation, migration and tenogenic differentiation of stem cells in vitro. Furthermore, the scaffolds promote endogenous stem cell recruitment to the defects, facilitate collagen deposition and improve the biomechanics of injured tendons, thus resulting in functional regeneration of tendons.

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从肌腱来源的干细胞中装载胶原靶向细胞外囊泡的脱细胞肌腱支架促进肌腱再生

基于干细胞的肌腱损伤治疗仍然有一些固有的问题。来自干细胞的细胞外囊泡在肌腱再生中显示出有希望的成就，尽管它们在体内的保留率很低。本研究报道了利用胶原结合域(CBD)将从肌腱源性干细胞(tdsc)中获得的细胞外囊泡与胶原结合。将cbd -细胞外囊泡(cbd - ev)与脱细胞牛腱片(DBTS)偶联，制备生物功能化支架(cbd - ev -DBTS)。结果表明，生物功能化支架可促进体外干细胞的增殖、迁移和成腱分化。此外，支架可促进内源性干细胞向缺损处募集，促进胶原沉积，改善损伤肌腱的生物力学，从而实现肌腱的功能性再生。

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来源期刊

Journal of Controlled Release 医学-化学综合

CiteScore

18.50

自引率

5.60%

发文量

700

审稿时长

39 days

期刊介绍： The Journal of Controlled Release (JCR) proudly serves as the Official Journal of the Controlled Release Society and the Japan Society of Drug Delivery System. Dedicated to the broad field of delivery science and technology, JCR publishes high-quality research articles covering drug delivery systems and all facets of formulations. This includes the physicochemical and biological properties of drugs, design and characterization of dosage forms, release mechanisms, in vivo testing, and formulation research and development across pharmaceutical, diagnostic, agricultural, environmental, cosmetic, and food industries. Priority is given to manuscripts that contribute to the fundamental understanding of principles or demonstrate the advantages of novel technologies in terms of safety and efficacy over current clinical standards. JCR strives to be a leading platform for advancements in delivery science and technology.

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