Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-08-11 DOI:10.1262/jrd.2023-022

Madalitso Chelenga, Yojiro Yanagawa, Seiji Katagiri, Masashi Nagano

引用次数: 0

Abstract

In this study, we evaluated the effects of pre-maturational culture (pre-IVM) on the developmental competence of bovine oocytes derived from an 8-day in vitro growth (IVG) culture system. IVG oocytes were subjected to 5 h pre-IVM before in vitro maturation, followed by in vitro fertilization (IVF). The proportion of oocytes that progressed to the germinal vesicle breakdown stage was similar between groups with and without pre-IVM. Although metaphase II oocytes and cleavage rates after IVF were similar regardless of pre-IVM culture, the blastocyst rate was significantly higher in the group with pre-IVM (22.5%) than without pre-IVM (11.0%, P < 0.05). In conclusion, pre-IVM culture improved the developmental competence of bovine oocytes derived from an 8-day IVG system.

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在体外培养8天的牛卵母细胞中，预成熟培养可促进其发育能力。

在这项研究中，我们评估了预成熟培养(预ivm)对牛卵母细胞发育能力的影响，这些卵母细胞来自8天的体外生长(IVG)培养系统。IVG卵母细胞在体外成熟前进行5h的预ivm，然后进行体外受精(IVF)。预ivm组和未预ivm组的卵母细胞进入生发囊泡破裂期的比例相似。尽管体外受精后中期卵母细胞和卵裂率与体外受精前后无明显差异，但体外受精组的囊胚率(22.5%)显著高于未体外受精组(11.0%，P < 0.05)。综上所述，体外培养可提高体外培养8天的牛卵母细胞的发育能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.