Characterization of an EPS-producing bifidobacterial strain based on integration of phenotypic and complete genome sequencing data.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-10-01 Epub Date: 2023-06-23 DOI:10.1139/cjm-2023-0025

Dianbin Liu, Haohan Huang, Jinzhi Han, Qiong Wu, Yaoyao Xiang, Yan Liu, Yanxia Wei

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引用次数: 0

Abstract

Bifidobacterium and Lactobacillus are known to be common members of the human intestinal microbiota, which play important roles in maintaining the homeostasis of host gut microenvironment. Several bifidobacterial and lactobacilli strains have been used as probiotics for health benefits. The exopolysaccharides (EPSs) produced by strains from Bifidobacterium and Lactobacillus are considered as beneficial traits mediating these beneficial effects. In this study, 21 strains belonging to Bifidobacterium and Lactobacillus were isolated from healthy infants' stool and were screened for EPS-producing ability. Among these strains, Bifidobacterium longum XZM1 showed the highest EPS productivity, which was further confirmed and characterized. The complete genome of strain XZM1 was sequenced, which revealed the presence of a gene cluster for EPS production. Furthermore, comparative genome analysis was performed among XZM1 and other strains from B. longum species. Following purification, the molecular weight (Mw) of EPS from XZM1 was determined as 4023 Da (Mw) through gel permeation chromatography. Analysis of the EPS hydrolysates revealed that the EPS was composed of mannose, glucose, galactose, arabinose, and fucose. Additionally, the EPS exhibited higher scavenging abilities toward hydroxyl than 1,1-diphenyl-2-picrylhydrazyl free radical. Overall, these results suggest that XZM1 from B. longum species may be a promising probiotic candidate.

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基于表型和全基因组测序数据整合的产EPS双歧杆菌菌株的特征。

双歧杆菌和乳酸杆菌是人类肠道微生物群的常见成员，在维持宿主肠道微环境的稳态方面发挥着重要作用。一些双歧杆菌和乳酸杆菌菌株已被用作有益健康的益生菌。双歧杆菌和乳酸杆菌菌株产生的胞外多糖（EPSs）被认为是介导这些有益作用的有益性状。本研究从健康婴儿粪便中分离出21株双歧杆菌和乳酸杆菌，并对其产生EPS的能力进行了筛选。在这些菌株中，长双歧杆菌XZM1表现出最高的EPS生产力，这一点得到了进一步的证实和表征。对菌株XZM1的全基因组进行了测序，揭示了EPS生产的基因簇的存在。此外，在XZM1和来自B.longum物种的其他菌株之间进行了比较基因组分析。纯化后，通过凝胶渗透色谱法测定来自XZM1的EPS的分子量（Mw）为4023Da（Mw。对EPS水解产物的分析表明，EPS由甘露糖、葡萄糖、半乳糖、阿拉伯糖和岩藻糖组成。此外，EPS对羟基的清除能力高于1，1-二苯基-2-苦基肼基自由基。总之，这些结果表明，来自B.longum物种的XZM1可能是一种有前途的益生菌候选物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.