Determination of Serum Differential Carnitine Ester Levels in HIV(+) Patients: A Cross-Sectional Study.

IF 0.8 4区医学 Q4 IMMUNOLOGY

Current HIV Research Pub Date : 2023-01-01 DOI:10.2174/1570162X21666221219141016

Irfan Binici, Halil İbrahim Akbay, Zübeyir Huyut, Hamit Hakan Alp, Hasan Karsen, Ismail Koyuncu, Ataman Gonel, Şükrü Akmeşe, Hakim Çelik

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引用次数: 0

Abstract

Objective: It has been reported that carnitine deficiency is observed in various viral infections and in the follow-up of the prognosis of some diseases. In this cross-sectional study, we aimed to determine how carnitine ester derivatives change in HIV-positive patients.

Materials and methods: In this study, 25 HIV-infected patients who applied to Harran University Faculty of Medicine Education Research and Practice Hospital Infectious Diseases and Clinical Microbiology Outpatient Clinic and who did not receive any antiretroviral treatment, as well as 25 healthy volunteers were included in the study. Carnitine ester levels in serum samples were measured by Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) method (Shimadzu North America, Columbia, MD, USA).

Results: While suberoylcarnitine (C8DC), myristoleylcarnitine (C14:1), tetradecadienoylcarnitine (C14:2), palmitoleylcarnitine (C16:1), and linoleylcarnitine (C18:2) levels in HIV(+) patients were quite low compared to the control group, tiglylcarnitine (C5:1) levels were high (p ≤ 0.05). In addition, C5:1 and C14:2 index parameters according to VIP score, and C5:1 and C14:1/C16 index parameters according to ROC analysis were determined as markers with high potential to distinguish HIV(+) patients from healthy volunteers.

Conclusion: This study showed that levels of acylcarnitine derivatives might be altered in HIV(+) patients, and the results obtained may contribute to a better understanding of carnitine metabolism.

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HIV(+)患者血清肉碱酯水平差异的测定:一项横断面研究。

目的:在各种病毒感染和某些疾病预后随访中均可观察到肉碱缺乏症。在这项横断面研究中，我们旨在确定肉毒碱酯衍生物在hiv阳性患者中的变化。材料与方法:本研究纳入25名申请到哈兰大学医学院教育研究与实践医院传染病与临床微生物学门诊未接受任何抗逆转录病毒治疗的hiv感染者，以及25名健康志愿者。采用液相色谱-质谱/质谱(LC-MS/MS)法测定血清样品中的肉碱酯水平(Shimadzu North America, Columbia, MD, USA)。结果:HIV(+)患者亚硝基肉碱(C8DC)、肉豆醇基肉碱(C14:1)、十四二烯基肉碱(C14:2)、棕榈油基肉碱(C16:1)、亚油基肉碱(C18:2)水平较对照组低，而甲酰肉碱(C5:1)水平较高(p≤0.05)。此外，根据VIP评分确定C5:1和C14:2指标参数，根据ROC分析确定C5:1和C14:1/C16指标参数是区分HIV(+)患者与健康志愿者的高潜力标志物。结论:本研究表明，在HIV(+)患者中酰基肉碱衍生物的水平可能会发生改变，所得结果可能有助于更好地了解肉碱代谢。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current HIV Research 医学-病毒学

CiteScore

1.90

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Current HIV Research covers all the latest and outstanding developments of HIV research by publishing original research, review articles and guest edited thematic issues. The novel pioneering work in the basic and clinical fields on all areas of HIV research covers: virus replication and gene expression, HIV assembly, virus-cell interaction, viral pathogenesis, epidemiology and transmission, anti-retroviral therapy and adherence, drug discovery, the latest developments in HIV/AIDS vaccines and animal models, mechanisms and interactions with AIDS related diseases, social and public health issues related to HIV disease, and prevention of viral infection. Periodically, the journal invites guest editors to devote an issue on a particular area of HIV research of great interest that increases our understanding of the virus and its complex interaction with the host.