A Validated High-Performance Thin-Layer Chromatography Technique for Routine Analysis of Curcumin in Four Different Species of Curcuma Viz. C. amada, C. caesia, C. longa and C. zedoaria.

IF 1.5 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of chromatographic science Pub Date : 2025-02-08 DOI:10.1093/chromsci/bmad063

Avinash Gangal, Manisha Duseja, Neeraj K Sethiya, Dheeraj Bisht, Sushil Kumar Chaudhary, Vijay Singh Rana

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Abstract

In this study, we investigated a new, simple, sensitive, selective and precise high-performance thin-layer chromatography (HPTLC) fingerprint and quantitative estimation method for the routine analysis of curcumin in Curcuma species viz. Curcuma amada, Curcuma caesia, Curcuma longa and Curcuma zedoaria. Linear ascending development was carried out in a twin-trough glass chamber saturated with toluene:acetic acid (4:1; v/v with 20 minutes of saturation). The plate was dried and analyzed by CAMAG TLC scanner III at white light and 366 nm. The system was found to give compact spots for curcumin (Rf 0.42). The relationship between the concentration of standard solutions and the peak response is linear within the concentration range of 10-70 ng/spot for curcumin. In result, curcumin was not detected in any of C. caesia extracts. The percentage of curcumin was found between 0.042 and 4.908 (%w/w) in different Curcuma species obtained by two different extraction methods viz. Soxhlet and sonication, respectively. Further, extraction via Soxhlet method is most suitable method to get higher curcumin content from rhizomes. The proposed HPTLC method may be use for routine quality testing and quantification of curcumin in Curcuma samples.

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高效薄层色谱法测定四种姜黄中姜黄素的含量

本研究建立了一种简便、灵敏、选择性和精确的高效薄层色谱（HPTLC）指纹图谱和定量分析方法，用于姜黄、黄姜黄、长姜黄和莪术中姜黄素的常规分析。在饱和甲苯：醋酸(4:1；V / V， 20分钟饱和)。干燥后用CAMAG TLC III扫描仪在白光和366nm下进行分析。该体系对姜黄素有致密的斑点（Rf 0.42）。姜黄素浓度在10 ~ 70 ng/spot范围内，标准溶液浓度与峰响应呈线性关系。结果表明，莪术提取物中均未检测到姜黄素。索氏法和超声法提取的姜黄素含量在0.042 ~ 4.908 （%w/w）之间。此外，索氏法提取是获得较高姜黄素含量的最佳方法。该方法可用于姜黄样品中姜黄素的常规质量检测和定量。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.