Stromal Cells Associated with Soft Tissue Augmentation by a Volume-Stable Collagen Matrix (VCMX) Are Predominated by Anti-inflammatory/Reparative Macrophages.

IF 1.3 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Zvi Artzi, Marilena Vered, Tal Maymon-Gil, Uri Renert, Erez Netanely, Daniel S Thoma
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Abstract

This article defines immunophenotypes of stromal inflammatory and endothelial cells and fibroblasts 3 months after augmentation of the peri-implant soft tissue using a porcine cross-linked collagen matrix (VCMX). Peri-implant soft tissue samples were obtained from 12 patients at the lining mucosa (LM)-masticatory mucosa (MM) junction before (1) and 3 months after (2) augmentation. Immu- nohistochemical stains were performed to identify inflammatory cells (T [CD3] and B [CD20] lym- phocytes, plasma cells [CD138]), macrophages (CD68-proinflammatory, CD163-anti-inflammatory/ reparative), endothelial cells (CD31, CD34), and fibroblasts (CD90, TE-7). Differences in the mean positively stained cells pre- and postaugmentation were analyzed by Wilcoxon signed-rank test. CD31+ endothelial cells showed increased mean numbers in MM2 compared to MM1 (P = .025) and in LM2 compared to LM1 (P = .047). CD163+ anti-inflammatory macrophages showed higher mean numbers in MM2 than in MM1 (P = .021) and in LM2 than in LM1 (P = .012). All other cell phenotypes showed nonsignificant changes between pre- and postaugmentation. This molecular study provides novel insight on the frequency of stromal cell phenotypes in the wound healing process at 3 months postaugmentation with VCMX, with anti-inflammatory CD163+ macrophages being predominant. This should be further investigated to help find novel therapeutic approaches to modulate and promote the VCMX-related healing process.

与体积稳定胶原基质(VCMX)软组织增生相关的基质细胞主要是抗炎/修复巨噬细胞。
目的:使用猪交联胶原基质(VCMX)确定种植体周围软组织增量后 3 个月的基质炎症细胞、内皮细胞和成纤维细胞的免疫表型:从 12 名患者的种植体周围粘膜(LM)-咀嚼粘膜(MM)交界处采集种植体周围软组织样本,分别在种植体植入前和植入后 3 个月采集。采用免疫组化染色法鉴定炎症细胞[T(CD3)和 B(CD20)淋巴细胞、浆细胞(CD138)]、巨噬细胞(CD68-前炎症性、CD163-抗炎症/修复性)、内皮细胞(CD31、CD34)和成纤维细胞(CD90、TE-7)。通过 Wilcoxon Signed-Rank 检验分析了增强前后平均阳性染色细胞的差异:CD31+内皮细胞的平均数量在 MM2 中比 MM1 中增加(p=0.025),在 LM2 中比 LM1 中增加(p=0.047)。CD163+抗炎巨噬细胞在 MM2 中的平均数量高于 MM1(p=0.021),在 LM2 中的平均数量高于 LM1(p=0.012)。所有其他细胞表型在增强前后的变化均不显著:这项分子研究为 VCMX 增强 3 个月后伤口愈合过程中基质细胞表型的频率提供了新的见解,其中以抗炎性 CD163+ 巨噬细胞为主。这一点应进一步研究,以便找到新的治疗方法来调节和促进与 VCMX 相关的愈合过程。
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来源期刊
CiteScore
2.90
自引率
6.20%
发文量
113
审稿时长
6-12 weeks
期刊介绍: The International Journal of Periodontics & Restorative Dentistry will publish manuscripts concerned with all aspects of clinical periodontology, restorative dentistry, and implantology. This includes pertinent research as well as clinical methodology (their interdependence and relationship should be addressed where applicable); proceedings of relevant symposia or conferences; and quality review papers. Original manuscripts are considered for publication on the condition that they have not been published or submitted for publication elsewhere.
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