Quantitative analysis of lncRNA in formalin-fixed paraffin-embedded tissues of oral squamous cell carcinoma.

IF 2.2 4区工程技术 Q3 BIOCHEMICAL RESEARCH METHODS

BioTechniques Pub Date : 2023-10-01 Epub Date: 2023-08-17 DOI:10.2144/btn-2023-0033

Kiran Kumar, Kaveri Hallikeri, Ajaykumar Oli, Mallikarjun Goni, Apoorva Jain, Jagadeesha Poyya, Alagilavada S Shilpasree, Palaksha Kanive Javaregowda

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引用次数: 0

Abstract

The study evaluated expression profiles of few regulatory lncRNAs in oral squamous cell carcinoma and normal mucosa adjacent to oral cancer using paired fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues stored at a different duration of time (1-5 years) using real-time quantitative PCR. The quantity and quality of total RNA isolated from FFPE tissues was less compared with that of fresh frozen tissues, which resulted in a noncorrelation of quantification cycle values. Following normalization, the expression of lncRNAs in the paired tissues did not differ significantly. The differential expression of the lncRNAs in the study was consistent with The Cancer Genome Atlas head and neck squamous cell carcinoma database. The study findings demonstrate the possibility of performing accurate quantitative analysis of lncRNAs using short amplicons and standardized real-time quantitative PCR assays in oral squamous cell carcinoma FFPE samples.

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口腔鳞状细胞癌福尔马林固定石蜡包埋组织中lncRNA的定量分析。

该研究使用实时定量PCR，使用储存在不同时间（1-5年）的成对新鲜冷冻和甲酰固定石蜡包埋（FFPE）组织，评估了口腔鳞状细胞癌和口腔癌症附近正常粘膜中少数调节lncRNA的表达谱。与新鲜冷冻组织相比，从FFPE组织中分离的总RNA的数量和质量较低，这导致定量周期值不相关。正常化后，配对组织中lncRNA的表达没有显著差异。研究中lncRNA的差异表达与癌症基因组图谱头颈部鳞状细胞癌数据库一致。研究结果证明了在口腔鳞状细胞癌FFPE样本中使用短扩增子和标准化实时定量PCR分析对lncRNA进行准确定量分析的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BioTechniques 工程技术-生化研究方法

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

3.3 months

期刊介绍： BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies. Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers: Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices Surveys of technical approaches related to broad fields of research Reviews discussing advancements in techniques and methods related to broad fields of research Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.