Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture.

IF 1 Q4 NEUROSCIENCES

Basic and Clinical Neuroscience Pub Date : 2022-09-01 DOI:10.32598/bcn.2021.973.3

Ardeshir Moayeri, Rafieh Alizadeh, Hatef Ghasemi Hamidabadi, Maryam Nazm Bojnordi, Ali Niapour, Azim Hedayatpour, Marzieh Darvishi, Fatemeh Heidari, Maryam Soleimani, Leila Elyasi

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引用次数: 1

Abstract

Introduction: The induction of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in tissue engineering and experimental and clinical treatments of various neurodegenerative diseases, including Parkinson disease. This study aims to differentiate HUC-MSCs into dopaminergic neuron-like cells.

Methods: Following the isolation and characterization of HUC-MSCs, they were transferred to Matrigel-coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. The capacity of differentiation into dopaminergic neuron-like cells in 2-dimensional culture and on Matrigel was assessed by real-time polymerase chain reaction, immunocytochemistry, and high-performance liquid chromatography.

Results: Our results showed that dopaminergic neuronal markers' transcript and protein levels were significantly increased on the Matrigel differentiated cells compared to 2D culture plates.

Conclusion: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potential for the treatment of dopaminergic neuron-related diseases.

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人脐带来源的间充质干细胞在多巴胺能神经元中的三维培养中的转分化。

引言：人类脐带来源的间充质干细胞（HUC-MSCs）向多巴胺能神经元的诱导是组织工程以及包括帕金森病在内的各种神经退行性疾病的实验和临床治疗中的一个重大挑战。本研究旨在将HUC间充质干细胞分化为多巴胺能神经元样细胞。方法：在分离和表征HUC-MSCs后，将其转移到Matrigel包被的平板上，并与多巴胺能神经元分化因子的混合物孵育。通过实时聚合酶链反应、免疫细胞化学和高效液相色谱法评估在二维培养基和基质胶上分化为多巴胺能神经元样细胞的能力。结果：我们的结果显示，与2D培养板相比，Matrigel分化的细胞上多巴胺能神经元标记物的转录物和蛋白质水平显著增加。结论：总之，本研究结果表明，HUC-MSCs在Matrigel上可以成功分化为多巴胺能神经元样细胞，在治疗多巴胺能神经元相关疾病方面具有巨大潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Basic and Clinical Neuroscience NEUROSCIENCES-

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

4 weeks

期刊介绍： BCN is an international multidisciplinary journal that publishes editorials, original full-length research articles, short communications, reviews, methodological papers, commentaries, perspectives and “news and reports” in the broad fields of developmental, molecular, cellular, system, computational, behavioral, cognitive, and clinical neuroscience. No area in the neural related sciences is excluded from consideration, although priority is given to studies that provide applied insights into the functioning of the nervous system. BCN aims to advance our understanding of organization and function of the nervous system in health and disease, thereby improving the diagnosis and treatment of neural-related disorders. Manuscripts submitted to BCN should describe novel results generated by experiments that were guided by clearly defined aims or hypotheses. BCN aims to provide serious ties in interdisciplinary communication, accessibility to a broad readership inside Iran and the region and also in all other international academic sites, effective peer review process, and independence from all possible non-scientific interests. BCN also tries to empower national, regional and international collaborative networks in the field of neuroscience in Iran, Middle East, Central Asia and North Africa and to be the voice of the Iranian and regional neuroscience community in the world of neuroscientists. In this way, the journal encourages submission of editorials, review papers, commentaries, methodological notes and perspectives that address this scope.