Preparation, Physicochemical Characterization, and Stability Study of Lippia origanoides Essential Oil-based Nanoemulsion as a Topical Delivery System.

Q2 Pharmacology, Toxicology and Pharmaceutics
Carlos Andrés Benitez-Llano, Oscar Albeiro Florez-Acosta, Darsy Dayana Velasquez-Polo, Ana Cecilia Mesa-Arango, Carolina Zapata-Zapata
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引用次数: 0

Abstract

Introduction: Fungal diseases are a priority in research, development, and health care, according to the WHO, mainly due to Candida spp. Essential oils (EOs) of the genus Lippia have demonstrated broad antimicrobial biological activity. Previous studies identified the anti-Candida potential of a thymol/p-cymene chemotype EO from Lippia origanoides H.B.K coded "0018". Nanoemulsions favor the biological activity of EOs and overcome limitations such as low solubility, instability against oxidizing agents, pH, light, and low permeability. To develop, characterize, and adjust a prototype of an O/W nanoemulsion containing the "0018" EO from Lippia origanoides for its evaluation in an in vitro permeability study.

Methods: Nanoemulsions were obtained using a high energy high shear method. Their particle size distribution, Z potential, viscosity, pH, encapsulation efficiency (EE), thermodynamic stability and the Turbiscan Stability Index (TSI) were evaluated. The nanoemulsion prototype was adjusted to improve performance characteristics and microbiological efficacy. Thymol was used as an analyte in the EO quantification using UHPLC-DAD.

Results: An O/W nanoemulsion with hydrodynamic diameter <200 nm and polydispersity index <0.3, EE >95%, with TSI < 1.5, anti-Candida albicans efficiency >95% was obtained; permeable with a flow of 6.0264 μg/cm2/h and permeability coefficient of 1.3170x10-3 cm/h.

Conclusion: A pharmaceutical formulation prototype is obtained that maintains the physical and physicochemical characteristics over time. Permeability is verified in an in-vitro model. It is proposed to evaluate its antifungal activity in preclinical or clinical studies as a contribution to the treatment of topical fungal diseases caused by Candida spp., through the use of biological resources and Colombian biodiversity.

作为局部给药系统的麝香石竹精油基纳米乳液的制备、理化特性和稳定性研究
导言:世界卫生组织认为,真菌疾病是研究、开发和医疗保健的重点,主要是由念珠菌属引起的。先前的研究发现,来自 Lippia origanoides H.B.K 的一种百里酚/p-胸腺嘧啶化学型精油(编码为 "0018")具有抗念珠菌的潜力。纳米乳剂有利于提高环氧乙烷的生物活性,并克服了低溶解度、对氧化剂不稳定、pH 值、光和低渗透性等局限性。目的:开发、表征和调整一种含麝香草 "0018 "环氧乙烷的 O/W 纳米乳液原型,以便在体外渗透性研究中对其进行评估:方法:采用高能高剪切法获得纳米乳液。方法:采用高能高剪切法获得纳米乳液,并对其粒度分布、Z 电位、粘度、pH 值、封装效率(EE)、热力学稳定性和 Turbiscan 稳定指数(TSI)进行了评估。对纳米乳液原型进行了调整,以改善其性能特征和微生物功效。使用 UHPLC-DAD 对百里酚作为环氧乙烷的分析物进行定量:结果:获得了一种水动力直径为 95%、TSI < 1.5、抗白色念珠菌效率大于 95% 的 O/W 纳米乳液;渗透流量为 6.0264 μg/cm2/h,渗透系数为 1.3170x10-3 cm/h:结论:获得的药物制剂原型可长期保持物理和理化特性。在体外模型中验证了渗透性。建议在临床前或临床研究中评估其抗真菌活性,通过利用生物资源和哥伦比亚的生物多样性,为治疗由念珠菌属引起的局部真菌疾病做出贡献。
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来源期刊
Pharmaceutical nanotechnology
Pharmaceutical nanotechnology Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
4.20
自引率
0.00%
发文量
46
期刊介绍: Pharmaceutical Nanotechnology publishes original manuscripts, full-length/mini reviews, thematic issues, rapid technical notes and commentaries that provide insights into the synthesis, characterisation and pharmaceutical (or diagnostic) application of materials at the nanoscale. The nanoscale is defined as a size range of below 1 µm. Scientific findings related to micro and macro systems with functionality residing within features defined at the nanoscale are also within the scope of the journal. Manuscripts detailing the synthesis, exhaustive characterisation, biological evaluation, clinical testing and/ or toxicological assessment of nanomaterials are of particular interest to the journal’s readership. Articles should be self contained, centred around a well founded hypothesis and should aim to showcase the pharmaceutical/ diagnostic implications of the nanotechnology approach. Manuscripts should aim, wherever possible, to demonstrate the in vivo impact of any nanotechnological intervention. As reducing a material to the nanoscale is capable of fundamentally altering the material’s properties, the journal’s readership is particularly interested in new characterisation techniques and the advanced properties that originate from this size reduction. Both bottom up and top down approaches to the realisation of nanomaterials lie within the scope of the journal.
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