欧洲地松鼠与北美地松鼠没有相同的隐孢子虫属。

IF 1.5 4区 医学 Q3 PARASITOLOGY
Jana Jezkova, Nikola Holubova, Jana Kvicerova, Jan Mateju, Bohumil Sak, Martin Kvac
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引用次数: 0

摘要

泰泽隐孢子虫(crypsporidium Tyzzer)是脊椎动物中最常见的原生寄生虫之一。本研究结果首次提供了欧洲地松鼠(spermoophilus citellus)隐孢子虫多样性的资料。对来自捷克共和国39个地区的128份欧洲地松鼠粪便样本进行了隐孢子虫的镜检和小亚基核糖体RNA (SSU)及肌动蛋白基因的PCR/序列分析。虽然显微镜检查未发现任何隐孢子虫卵囊的存在,但来自6个地点的8个样本pcr阳性。系统发育分析显示存在5种不同的隐孢子虫分离株。在野生种群中检测到的4个分离株,被命名为隐孢子虫Sc01-04,与之前在北美地松鼠物种中发现的隐孢子虫基因型密切相关。隐孢子虫(crypsporidium sciurinum Prediger), Ježková, holubov, Sak, Konečný, Rost, McEvoy, Rajský et Kváč, 2021年在动物保护区发现。由于在同一地点曾在欧亚红松鼠(Sciurus vulgaris Linnaeus)中检测到隐孢子虫,因此可以认为该隐孢子虫是在动物保护区内由树松鼠传播给地松鼠的。结果表明,欧洲和北美地松鼠种群中存在不同隐孢子虫,同时首次描述了隐孢子虫在地松鼠中的发生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
European ground squirrels Spermophilus citellus (Linnaeus) do not share identical Cryptosporidium spp. with North American ground squirrels.

Cryptosporidium Tyzzer, 1910 is one of the most common protistan parasites of vertebrates. The results of this study provide the first data on Cryptosporidium diversity in the European ground squirrel Spermophilus citellus (Linnaeus). A total of 128 faecal samples of European ground squirrels from 39 localities in the Czech Republic were analysed for the presence of Cryptosporidium spp. by microscopy and PCR/sequence analysis of small subunit ribosomal RNA (SSU) and the actin gene. While the microscopical examination did not reveal the presence of any Cryptosporidium oocysts, eight samples from six localities were PCR-positive. Phylogenetic analyses revealed the presence of five different Cryptosporidium spp. isolates. Four isolates, designated as Cryptosporidium sp. isolate Sc01-04, detected in wild populations and never recorded before, clustered closely to Cryptosporidium genotypes that have previously been found in North American ground squirrels' species. Cryptosporidium sciurinum Prediger, Ježková, Holubová, Sak, Konečný, Rost, McEvoy, Rajský et Kváč, 2021 was found in an animal sanctuary. Because C. sciurinum had previously been detected in Eurasian red squirrels Sciurus vulgaris Linnaeus at the same facility, it can be concluded that this Cryptosporidium was transmitted from tree squirrels to ground squirrels within the animal sanctuary. The results indicate that populations of European and North American ground squirrels are parasitised by different Cryptosporidium spp. At the same time, this is the first description of the occurrence of C. sciurinum in ground squirrels.

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来源期刊
Folia Parasitologica
Folia Parasitologica 医学-寄生虫学
CiteScore
2.70
自引率
0.00%
发文量
25
审稿时长
>12 weeks
期刊介绍: FOLIA PARASITOLOGICA, issued in online versions, is an international journal that covers the whole field of general, systematic, ecological and experimental parasitology. It publishes original research papers, research notes and review articles. Contributions from all branches of animal parasitology, such as morphology, taxonomy, biology, biochemistry, physiology, immunology, molecular biology and evolution of parasites, and host-parasite relationships, are eligible. Novelty and importance in the international (not local or regional) context are required. New geographical records of parasites, records of new hosts, regional parasite and/or host surveys (if they constitute the principal substance of manuscript), local/regional prevalence surveys of diseases, local/regional studies on epidemiology of well known diseases and of parasite impact on human/animal health, case reports, routine clinical studies and testing of established diagnostic or treatment procedures, will not be considered. One species description will also not be considered unless they include more general information, such as new diagnostic characters, host-parasite associations, phylogenetic implications, etc. Manuscripts found suitable on submission will be reviewed by at least two reviewers.
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