新型肺炎链球菌血清型7D的简易PCR鉴定。

IF 2.4 4区医学 Q3 MICROBIOLOGY

Journal of medical microbiology Pub Date : 2023-08-01 DOI:10.1099/jmm.0.001739

Karsten Maruhn, Andreas Itzek, Matthias Imoehl, Mark van der Linden

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引用次数: 0

摘要

通过纽菲尔德平息反应鉴定新型肺炎球菌血清型7D需要大量的专业知识。为了避免这种情况，我们开发了一种简单的血清型特异性PCR方法来区分血清型7D与密切相关的血清型7C, 7B和40。用德国国家链球菌参考中心(GNRCS)收集的菌株对建立的PCR进行验证。然而，没有一株最初被鉴定为血清型7B、7C或40的分离株被鉴定为血清型7D。

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A simple PCR assay for the identification of the novel Streptococcus pneumoniae serotype 7D.

The identification of the novel pneumococcal serotype 7D by Neufeld quellung reaction requires significant expertise. To circumvent this, we developed a simple serotype-specific PCR method to discriminate serotype 7D from the closely related serotypes 7C, 7B and 40. The established PCR was validated with the strain collection of the German National Reference Center for Streptococci (GNRCS). However, no isolate initially assigned as serotype 7B, 7C or 40 was identified as serotype 7D.

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来源期刊

Journal of medical microbiology 医学-微生物学

CiteScore

5.50

自引率

3.30%

发文量

143

审稿时长

4.5 months

期刊介绍： Journal of Medical Microbiology provides comprehensive coverage of medical, dental and veterinary microbiology, and infectious diseases. We welcome everything from laboratory research to clinical trials, including bacteriology, virology, mycology and parasitology. We publish articles under the following subject categories: Antimicrobial resistance; Clinical microbiology; Disease, diagnosis and diagnostics; Medical mycology; Molecular and microbial epidemiology; Microbiome and microbial ecology in health; One Health; Pathogenesis, virulence and host response; Prevention, therapy and therapeutics