类风湿关节炎患者甲氨蝶呤治疗前后血清细胞因子的变化。

IF 1.9 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Manoj Lama, Rajat Sarkar, Bappaditya Ghosh
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引用次数: 0

摘要

类风湿性关节炎(RA)是一种影响全球约1%人口的炎症性自身免疫性疾病。细胞因子在RA的发病机制中起着至关重要的作用。本研究的目的是比较甲氨蝶呤治疗组和甲氨蝶呤初治组、甲氨蝶呤治疗组和健康对照组、甲氨蝶呤初治组和健康对照组之间的血清细胞因子谱。采用酶联免疫吸附试验(ELISA)试剂盒定量测定80例RA患者(48例MTX治疗组和32例MTX初发组)和80例健康对照者血清中肿瘤坏死因子-α (TNF-α)、白细胞介素(IL)-1β、IL-17、IL-6、干扰素-γ (IFN-γ)和IL-10的浓度。对于所有细胞因子的测定,在450nm处使用微孔板阅读器(Bio-Rad)测量吸光度。采用独立样本t检验比较各组血清细胞因子浓度,采用SPSS版本25。mtx治疗组RA患者血清TNF-α(36.13±17.64比45.82±23.07,*P = 0.037)、IL-17(307.85±151.74比435.42±241.19,**P = 0.006)、IFN-γ(414.93±212.13比527.15±269.61,*P = 0.041)水平明显低于mtx治疗组RA患者。与健康对照组相比,mtx治疗组和mtx初发RA患者组血清TNF-α、IL-1β、IL-17、IL-6、IFN-γ和IL-10水平均显著升高(***P
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Serum Cytokine Profiles in Patients with Rheumatoid Arthritis Before and After Treatment with Methotrexate.

Rheumatoid arthritis (RA) is an inflammatory autoimmune illness affecting around 1% of the population globally. Cytokines have a crucial role in the pathogenesis of RA. The objectives of the present study were to compare the serum cytokine profiles between methotrexate (MTX)-treated and MTX-naive RA patient groups, MTX-treated RA patient group and healthy controls, and MTX-naive RA patient group and healthy controls. Enzyme linked immunosorbent assay (ELISA) kits were used to quantify the serum concentrations of tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, IL-17, IL-6, interferon-gamma (IFN-γ), and IL-10 in 80 RA patients (48 MTX treated and 32 MTX naive) and 80 healthy controls. For all cytokine assays, absorbance was measured at 450 nm using a microplate reader (Bio-Rad). Independent sample t-test was used to compare the serum cytokine concentrations between the study groups using SPSS version 25. MTX-treated RA patient group had significantly reduced serum levels of TNF-α (36.13 ± 17.64 versus 45.82 ± 23.07, *P = 0.037), IL-17 (307.85 ± 151.74 versus 435.42 ± 241.19, **P = 0.006), and IFN-γ (414.93 ± 212.13 versus 527.15 ± 269.61, *P = 0.041) compared to MTX-naive RA patients. Both MTX-treated and MTX-naive RA patient groups had significantly high serum levels of TNF-α, IL-1β, IL-17, IL-6, IFN-γ, and IL-10 when compared to healthy controls (***P < 0.001). Downregulation of the serum concentrations of certain key cytokines, viz. TNF-α, IL-17, and IFN-γ, demonstrates the anti-inflammatory effect of MTX in RA patients.

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来源期刊
CiteScore
3.80
自引率
0.00%
发文量
78
审稿时长
2.2 months
期刊介绍: Journal of Interferon & Cytokine Research (JICR) provides the latest groundbreaking research on all aspects of IFNs and cytokines. The Journal delivers current findings on emerging topics in this niche community, including the role of IFNs in the therapy of diseases such as multiple sclerosis, the understanding of the third class of IFNs, and the identification and function of IFN-inducible genes.
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