柔印工人细胞遗传损伤的评估:脱落颊上皮细胞的微核测定。

IF 0.8 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Parvatareddy Naveen Kumar, Bernard A Reginald, Basireddy Siva Reddy, Meda Samatha
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引用次数: 0

摘要

背景和目的:文献综述显示,印刷业中使用的化学品显示出遗传毒性和职业暴露之间的联系。柔性版印刷是最近流行的一种印刷技术,因为它具有快速、经济高效和高质量的标签印刷。微核(MN)被认为是遗传毒性损害的可靠标志物,通过确定染色体损伤的存在和程度,它与癌症发病率密切相关。由于没有关于柔性版印刷工人(FWs)的研究,本研究旨在分析和评估职业暴露对口腔上皮细胞MN频率的影响。材料和方法:该研究包括100名FW和100名有吸烟习惯和无吸烟习惯的年龄匹配的健康对照。使用细胞刷从所有受试者收集颊上皮细胞,然后用Feulgen快绿染色。使用Tolbert等人的标准记录每个个体的MN频率。采用单因素方差分析和事后检验对数据进行统计分析。结果:有吸烟习惯的FWs的MN频率(1.86±1.77)显著高于无吸烟习惯的工人(1.02±1.08)和对照组(有吸烟习惯1.26±1.33和无吸烟习惯0.62±0.92)。结论:本研究观察了FWs的细胞遗传学损伤,并得出结论,这些工人的遗传毒性风险更大,MN检测可以作为一种有用的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Assessment of Cytogenetic Damage in Flexographic Workers: Micronucleus Assay in Exfoliated Buccal Epithelial Cells.

Assessment of Cytogenetic Damage in Flexographic Workers: Micronucleus Assay in Exfoliated Buccal Epithelial Cells.

Background and objective: Literature review revealed that chemicals used in the printing industry show an association between genotoxicity and occupational exposure. Flexography is one type of printing technique recently becoming popular because of its fast, cost-efficient, and high-quality label printing. The micronucleus (MN) is considered to be a reliable marker for genotoxic damage, and it has a close association with cancer incidences by determining the presence and the extent of the chromosomal damage. Because there are no studies on flexographic workers (FWs), this study was intended to analyze and evaluate the effect of occupational exposure on the MN frequency of buccal epithelial cells.

Materials and methods: The study comprised 100 FWs and 100 age-matched healthy controls with and without smoking habits. Buccal epithelial cells were collected from all subjects by using a cytobrush, followed by staining with Feulgen fast green. The MN frequency was recorded for each individual using the Tolbert et al. criteria. Data was statistically analyzed by using one-way analysis of variance and the posthoc test.

Results: FWs with smoking habits showed a significant increase in MN frequency (1.86 ± 1.77) than workers without the habit (1.02 ± 1.08) and controls (with the habit 1.26 ± 1.33 and without the habit 0.62 ± 0.92). However, there was no significant increase of MN in FWs without the habit when compared with controls.

Conclusion: This study observed the cytogenetic damage in FWs and concludes that these workers are at greater risk for genotoxicity, and the MN assay can serve as a useful biomarker.

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来源期刊
Indian Journal of Occupational and Environmental Medicine
Indian Journal of Occupational and Environmental Medicine PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-
CiteScore
1.60
自引率
0.00%
发文量
25
期刊介绍: The website of Indian Journal of Occupational and Environmental Medicine aims to make the printed version of the journal available to the scientific community on the web. The site is purely for educational purpose of the medical community. The site does not cater to the needs of individual patients and is designed to support, not replace, the relationship that exists between a patient/site visitor and his/her existing physician.
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