MCM5是一种结肠癌的致癌基因,通过控制细胞周期促进肿瘤进展

IF 2.3 4区 生物学 Q4 CELL BIOLOGY
Jiayan Mao , Jian Shen , Xuemei Lu , Ying Cai , Rujia Tao , Yuqin Deng , Yuanting Zhang , Yuan Wu , Wei Chen
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引用次数: 0

摘要

许多结肠腺癌(COAD)患者被诊断为晚期,而COAD进展的分子机制是复杂和有争议的。因此,迫切需要鉴定更多新的COAD预后生物标志物,并阐明该疾病的分子机制。本研究旨在筛选出与COAD预后相关的关键基因。在本研究中,确定了一个关键模块,并选择了四个与COAD预后相关的枢纽基因(MCM5(编码微小染色体维持复合物组分5)、NOLC1(编码核仁和螺旋体磷蛋白1)、MYC(编码MYC原癌基因、BHLH转录因子)和CDK4(编码细胞周期蛋白依赖性激酶4),基于基因表达综合数据库中的GSE9348数据集。基因本体论富集和京都基因和基因组百科全书通路分析表明,MCM5与细胞周期相关。此外,根据各种数据库,包括癌症基因组图谱、临床蛋白质组肿瘤分析联盟数据库和人类蛋白质图谱数据库,与邻近组织相比,COAD患者的肿瘤组织中MCM5表达上调。小干扰RNA介导的MCM5敲除抑制了大肠癌癌症细胞的细胞周期和迁移。western印迹结果表明,在体外敲除MCM5后,与细胞周期相关的因子(CDK2/6、Cyclin D3、P21)表达下调。此外,在裸鼠模型中,MCM5的下调被证明可以抑制COAD的肺转移。总之,MCM5是COAD的致癌基因,通过细胞周期控制促进COAD的进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MCM5 is an oncogene of colon adenocarcinoma and promotes progression through cell cycle control

Many patients with colon adenocarcinoma (COAD) are diagnosed at an advanced stage, and the molecular mechanism of COAD progression is intricate and controversial. Therefore, there is an urgent need to identify more novel prognosis biomarkers for COAD and elucidate the molecular mechanism of this disease. The present study aimed to screen out key genes correlated with COAD prognosis. In this study, a key module was identified and four hub genes (MCM5 (encoding minichromosome maintenance complex component 5), NOLC1 (encoding nucleolar and coiled-body phosphoprotein 1), MYC (encoding MYC proto-oncogene, BHLH transcription factor), and CDK4 (encoding cyclin dependent kinase 4)) were selected that correlated with COAD prognosis, based on the GSE9348 dataset in Gene Expression Omnibus database. Gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that MCM5 correlated with the cell cycle. Furthermore, MCM5 expression was upregulated in tumor tissues of patients with COAD compared with that in adjacent tissues, based on various databases, including The Cancer Genome Atlas, the Clinical Proteomic Tumor Analysis Consortium database, and the Human Protein Atlas database. Small interfering RNA-mediated knockdown of MCM5 inhibited the cell cycle and migration of colorectal cancer cells in vitro. And western blotting results indicated that factors correlated with cell cycle (CDK2/6, Cyclin D3, P21) were downregulated after knockdown of MCM5 in vitro. Besides, downregulation of MCM5 was demonstrated to inhibit lung metastasis of COAD in nude mice model. In conclusion, MCM5 is an oncogene of COAD that promotes COAD progression via cell cycle control.

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来源期刊
Acta histochemica
Acta histochemica 生物-细胞生物学
CiteScore
4.60
自引率
4.00%
发文量
107
审稿时长
23 days
期刊介绍: Acta histochemica, a journal of structural biochemistry of cells and tissues, publishes original research articles, short communications, reviews, letters to the editor, meeting reports and abstracts of meetings. The aim of the journal is to provide a forum for the cytochemical and histochemical research community in the life sciences, including cell biology, biotechnology, neurobiology, immunobiology, pathology, pharmacology, botany, zoology and environmental and toxicological research. The journal focuses on new developments in cytochemistry and histochemistry and their applications. Manuscripts reporting on studies of living cells and tissues are particularly welcome. Understanding the complexity of cells and tissues, i.e. their biocomplexity and biodiversity, is a major goal of the journal and reports on this topic are especially encouraged. Original research articles, short communications and reviews that report on new developments in cytochemistry and histochemistry are welcomed, especially when molecular biology is combined with the use of advanced microscopical techniques including image analysis and cytometry. Letters to the editor should comment or interpret previously published articles in the journal to trigger scientific discussions. Meeting reports are considered to be very important publications in the journal because they are excellent opportunities to present state-of-the-art overviews of fields in research where the developments are fast and hard to follow. Authors of meeting reports should consult the editors before writing a report. The editorial policy of the editors and the editorial board is rapid publication. Once a manuscript is received by one of the editors, an editorial decision about acceptance, revision or rejection will be taken within a month. It is the aim of the publishers to have a manuscript published within three months after the manuscript has been accepted
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