睾丸特异性丝氨酸/苏氨酸激酶在树鼩生殖和非生殖季节的表达及其在成熟精子中的定位。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Comparative medicine Pub Date : 2023-08-27 Epub Date: 2023-08-07 DOI:10.30802/AALAS-CM-23-000043
Xia Tan, Xin Zhang, Xiang Li, Minghua Yang, Yahui Li
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引用次数: 0

摘要

树鼩表现出明显的繁殖周期,性成熟的雄性树鼩在非繁殖季节很少或不产生精子,精子活力极低;这种现象背后的机制仍然未知。由于睾丸特异性丝氨酸/苏氨酸激酶(TSSK)在哺乳动物的睾丸和雄性生殖细胞中特异性表达,我们假设它们可能在树鼩的精子发生或精子功能调节中发挥重要作用。此外,TSSK在树鼩精子中的表达、分布、亚细胞定位和动态变化尚不清楚。在这里,我们发现,在生殖季节,与非生殖季节相比,曲精小管明显更大,并且含有成熟的精子和其他生殖细胞。Tssk基因在睾丸中的mRNA表达显著高于其他组织,生殖季节睾丸中的信使核糖核酸水平显著高于非生殖季节。此外,Tssk3在睾丸和精子中的mRNA水平显著高于其他成员。具体而言,Tssk1mRNA分布在树鼩精子的顶体和整个鞭毛中,Tssk2存在于顶体中,Tssk3定位于顶体后区并在获能后重新定位到鞭毛的主要部分,Tssk6分布于顶体和顶体后区域。这些结果表明TSSK对树鼩的生殖功能具有重要的调控作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews (Tupaia belangeri).

Tree shrews display obvious reproductive cycles, and sexually mature male tree shrews produce little or no sperm with extremely low motility during the nonreproductive season; the mechanism underlying this phenomenon remains unknown. Because testis-specific serine/threonine kinases (TSSK) are specifically expressed in the testis and male germ cells of mammals, we hypothesized that they may have an important role in spermatogenesis or sperm function regulation in tree shrews. In addition, the expression, distribution, subcellular localization, and dynamic changes of TSSK in tree shrew sperm are unclear. Here we show that during the reproductive season, the seminiferous tubules were significantly larger as compared with the nonreproductive season and contained mature sperm and other germ cells. The mRNA expression of Tssk genes in testis was significantly higher than that in other tissues, and the mRNA level in the testis during the reproductive season was significantly higher than that in nonreproductive season. In addition, the mRNA level of Tssk3 in the testis and sperm was significantly higher than that of other members. Specifically, Tssk1 mRNA was distributed in the acrosome and throughout the flagellum of tree shrew sperm, Tssk2 was present in the acrosome, Tssk3 was localized to postacrosomal region and relocated to the main part of the flagellum after capacitation, and Tssk6 was distributed in the acrosome and postacrosomal region. These results indicate that the TSSK are important regulating reproductive function in tree shrews.

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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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