电刺激诱导出具有发育良好的神经网络和功能连通性的结构三维人体工程神经组织。

IF 3.7 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Xiaoting Meng, Xiyao Yu, Yingli Lu, Zhe Pei, Guoqiang Wang, Mingran Qi, Rongrong Liu, Jiaying Zhou, Xiaopin Guo, Zhengjie Zhou, Fang Wang
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引用次数: 0

摘要

目标。三维神经组织工程有望在开发神经疾病模型和功能替代品方面提供新的进展,以辅助中枢神经系统损伤的治疗。我们之前已经详细介绍了电刺激(ES)系统在体外产生3D小鼠工程神经组织(mENT)。然而,es诱导的人耳鼻喉癌(hENT)在结构和功能方面尚未被研究或鉴定。通过免疫荧光化学染色和电镜成像,研究了ES对(1)神经元分化和成熟、(2)神经突的生长和排列、(3)神经突触和髓鞘形成的影响。我们进一步研究了体外融合小鼠和人组织之间突触连接的形成。结果:es能诱导神经元分化,神经突有序生长,神经元亚型成熟,形成发育良好的神经网络,具有突触和髓鞘。最重要的是,我们发现细胞外K+浓度的升高导致hENT中神经元兴奋性的增加,这表明神经元细胞的电活动。意义。我们应用ES生成了有组织的3D hENT,并从结构和功能上对它们进行了鉴定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Electrical stimulation induced structural 3D human engineered neural tissue with well-developed neuronal network and functional connectivity.

Objective.Three-dimensional (3D) neural tissue engineering is expected to provide new stride in developing neural disease models and functional substitutes to aid in the treatment of central nervous system injury. We have previously detailed an electrical stimulation (ES) system to generate 3D mouse engineered neural tissue (mENT)in vitro. However, ES-induced human ENT (hENT) has not previously been either investigated or identified in structural and functional manner. Here, we applied ES as a stimulator to regulate human neural stem cells in 3D Matrigel, explored the components and functional properties of hENTs.Approach.By immunofluorescence chemical staining and electron microscope imaging, we evaluated the effects of ES on (1) neuronal differentiation and maturation, (2) neurites outgrowth and alignment in hENT, (3) formation of synapses and myelin sheaths in hENT. We further investigated the formation of synaptic connections betweenex-vivo-fused mouse and human tissue. We used calcium imaging to detect activities of neurons in hENT culture.Results.ES could induce neuronal differentiation, the orderly growth of neurites and the maturation of neuron subtypes to construct a well-developed neuronal network with synapses and myelin sheaths. Most importantly, we discovered that raising extracellular K+concentration resulted the increasing neuronal excitability in the hENT, indicating electrical activities in neuronal cells.Significance.We applied ES to generate the organised 3D hENTs and identified them in both structural and functional manner.

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来源期刊
Journal of neural engineering
Journal of neural engineering 工程技术-工程:生物医学
CiteScore
7.80
自引率
12.50%
发文量
319
审稿时长
4.2 months
期刊介绍: The goal of Journal of Neural Engineering (JNE) is to act as a forum for the interdisciplinary field of neural engineering where neuroscientists, neurobiologists and engineers can publish their work in one periodical that bridges the gap between neuroscience and engineering. The journal publishes articles in the field of neural engineering at the molecular, cellular and systems levels. The scope of the journal encompasses experimental, computational, theoretical, clinical and applied aspects of: Innovative neurotechnology; Brain-machine (computer) interface; Neural interfacing; Bioelectronic medicines; Neuromodulation; Neural prostheses; Neural control; Neuro-rehabilitation; Neurorobotics; Optical neural engineering; Neural circuits: artificial & biological; Neuromorphic engineering; Neural tissue regeneration; Neural signal processing; Theoretical and computational neuroscience; Systems neuroscience; Translational neuroscience; Neuroimaging.
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