Cu/Zn-SOD和Mn-SOD对UVC诱导的NIH/3T3细胞和小鼠皮肤损伤的保护作用

IF 2.3 4区 生物学 Q4 CELL BIOLOGY
Disi Chen , Xiaoyang Ai , Yang Li , Yue Li , Yunfan Ao , Jun Rong , Guopan Li
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引用次数: 1

摘要

超氧化物歧化酶(SOD)是一种具有多种金属辅因子的抗氧化酶,可以特异性清除活性氧(ROS),在各种紫外线诱导的损伤中发挥重要作用。因此,SOD具有抗紫外线辐射的作用。本研究的目的是比较不同金属辅因子Cu/Zn-SOD和Mn-SOD在抗紫外线辐射作用方面的差异。首先采用疏水相互作用色谱法和离子交换色谱法对SOD进行纯化。其次,采用甲基噻唑基二苯四唑溴化法和细胞衰老试剂盒研究了SOD对紫外线诱导的细胞损伤的保护作用。最后,通过组织病理学方法评价SOD对紫外线诱导的皮肤损伤的保护作用,并检测组织中丙二醛(MDA)和基质金属蛋白酶(MMPs)的表达水平。结果表明,Cu/Zn-SOD在促进细胞增殖、减轻细胞损伤、保护皮肤结构、调节MDA和MMPs表达水平等方面优于Mn-SOD,且无副作用。总之,Cu/Zn-SOD具有比Mn-SOD更好的抗紫外线辐射作用,可用于抗衰老和抗紫外线护肤品中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Protective effects of Cu/Zn-SOD and Mn-SOD on UVC radiation-induced damage in NIH/3T3 cells and murine skin

Protective effects of Cu/Zn-SOD and Mn-SOD on UVC radiation-induced damage in NIH/3T3 cells and murine skin

Superoxide dismutase (SOD) is an antioxidant enzyme with multiple metal cofactors that can specifically clear reactive oxygen species (ROS), which plays an important role in a variety of ultraviolet-induced lesions. Therefore, SOD has the anti-ultraviolet radiation effect. The objective of this study was to compare the differences in the anti-ultraviolet radiation effect of SOD with distinct metal cofactors: Cu/Zn-SOD and Mn-SOD. SOD was first purified using hydrophobic interaction chromatography and ion-exchange chromatography. Second, the Methylthiazolyldiphenyl-tetrazolium bromide method and cell senescence kits were used to study the protective effect of SOD on ultraviolet-induced cell damage. Finally, the protective effect of SOD on ultraviolet -induced skin damage was histopathologically evaluated, and the expression levels of malondialdehyde (MDA) and matrix metalloproteinases (MMPs) in tissues were detected. The results showed that Cu/Zn-SOD was superior to Mn-SOD in promoting cell proliferation, alleviating cell damage, protecting skin structure, and regulating the expression levels of MDA and MMPs, and it has no side effects. In conclusion, Cu/Zn-SOD had a better anti-ultraviolet radiation effect than Mn-SOD, and it can be used in anti-aging and anti-ultraviolet skin-care products.

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来源期刊
Acta histochemica
Acta histochemica 生物-细胞生物学
CiteScore
4.60
自引率
4.00%
发文量
107
审稿时长
23 days
期刊介绍: Acta histochemica, a journal of structural biochemistry of cells and tissues, publishes original research articles, short communications, reviews, letters to the editor, meeting reports and abstracts of meetings. The aim of the journal is to provide a forum for the cytochemical and histochemical research community in the life sciences, including cell biology, biotechnology, neurobiology, immunobiology, pathology, pharmacology, botany, zoology and environmental and toxicological research. The journal focuses on new developments in cytochemistry and histochemistry and their applications. Manuscripts reporting on studies of living cells and tissues are particularly welcome. Understanding the complexity of cells and tissues, i.e. their biocomplexity and biodiversity, is a major goal of the journal and reports on this topic are especially encouraged. Original research articles, short communications and reviews that report on new developments in cytochemistry and histochemistry are welcomed, especially when molecular biology is combined with the use of advanced microscopical techniques including image analysis and cytometry. Letters to the editor should comment or interpret previously published articles in the journal to trigger scientific discussions. Meeting reports are considered to be very important publications in the journal because they are excellent opportunities to present state-of-the-art overviews of fields in research where the developments are fast and hard to follow. Authors of meeting reports should consult the editors before writing a report. The editorial policy of the editors and the editorial board is rapid publication. Once a manuscript is received by one of the editors, an editorial decision about acceptance, revision or rejection will be taken within a month. It is the aim of the publishers to have a manuscript published within three months after the manuscript has been accepted
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