全基因组测序检测溶血葡萄球菌耐利奈唑胺多种机制。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Meerabai Manoharan, Ajit Ramesh Sawant, K Prashanth, Sujatha Sistla
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引用次数: 1

摘要

介绍。利奈唑胺是治疗多重耐药革兰氏阳性菌引起的严重感染的有效治疗选择。据报道,几种机制导致对这种抗生素产生耐药性。假设或缺口陈述。虽然已经报道了溶血葡萄球菌耐利奈唑胺的几种机制,但尚未完全确定耐药基因的流行程度和水平转移的潜力,特别是在印度的溶血葡萄球菌分离株中。目的:对耐利奈唑胺的溶血链球菌进行全基因组测序,以确定其耐药机制。对16株耐利奈唑胺溶血链球菌进行WGS检测,以检测cfr、optrA和poxtA基因的存在,以及23S rRNA和核糖体蛋白(L3、L4和L22)的突变,这些突变可能与利奈唑胺耐药机制有关。利用MLST finder识别序列类型。采用e检验法测定利奈唑胺的最低抑菌浓度(MIC)。采用聚合酶链反应(PCR)检测cfr基因。该研究记录了溶血链球菌耐利奈唑胺的三种不同机制。16株菌株中有13株对利奈唑胺表型耐药,其中12株cfr基因阳性。在大多数分离株中发现了23S rRNA的G2603T突变(n=13)。10株分离株存在L3核糖体蛋白R138V突变。12株cfr基因与G2603T或R138V突变组合的分离株显示出极高的MIC值。令人惊讶的是,发现三个表型敏感的分离株对cfr基因呈阳性,但对其他耐药机制呈阴性。重要的是,在几乎一半的分离株中,cfr基因存在于质粒上。ST3和ST1为优势序列型。所有表型耐药菌株均表现出两种或三种利奈唑胺耐药机制。在许多分离株的质粒上发现了cfr基因,表明其具有向更多致病生物水平转移的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multiple mechanisms of linezolid resistance in Staphylococcus haemolyticus detected by whole-genome sequencing.

Introduction. Linezolid is an effective therapeutic option for treating severe infections caused by multidrug-resistant Gram-positive organisms. Several mechanisms have been reported to be responsible for resistance to this antibiotic.Hypothesis or Gap Statement. Although several mechanisms of linezolid resistance have been reported in Staphylococcus haemolyticus, the prevalence and potential for horizontal transfer of resistance genes have not been fully characterized, particularly among S. haemolyticus isolates from India.Aim. To perform whole-genome sequencing (WGS) of linezolid-resistant S. haemolyticus isolates to characterize the resistance mechanisms.Methodology. WGS was performed for 16 linezolid-resistant S. haemolyticus isolates to check for the presence of cfr, optrA and poxtA genes and mutations in 23S rRNA and ribosomal proteins (L3, L4 and L22) that are possible mechanisms implicated in linezolid resistance. Sequence types were identified using MLST finder. The minimum inhibitory concentration (MIC) of linezolid was determined using the E-test method. Polymerase chain reaction (PCR) was carried out for the detection of the cfr gene.Results. The study documented three different mechanisms of linezolid resistance in S. haemolyticus. Thirteen of the 16 isolates were phenotypically resistant to linezolid, of which 12 were positive for the cfr gene. The G2603T mutation in 23S rRNA was found in the majority of the isolates (n=13). Ten isolates had the R138V mutation in L3 ribosomal protein. Twelve isolates with the cfr gene in combination with either G2603T or R138V mutations displayed extremely high MIC values. Surprisingly, three phenotypically sensitive isolates were found to be positive for the cfr gene but negative for other resistance mechanisms. Importantly, in almost half of the isolates the cfr gene was present on a plasmid. ST3 and ST1 were found to be the predominant sequence types.Conclusion. All phenotypically resistant isolates exhibited two or three linezolid resistance mechanisms. The cfr gene was found on plasmids in many isolates, demonstrating its potential for horizontal transfer to more pathogenic organisms.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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