局部交变电场对脑组织突触可塑性的调节。

IF 3.2 4区医学 Q2 ENGINEERING, BIOMEDICAL

Biomedical Engineering Letters Pub Date : 2023-06-30 eCollection Date: 2023-08-01 DOI:10.1007/s13534-023-00287-7

Chi Zhang, Yiqiang Li, Li Yang, Hongwei Zhao

{"title":"局部交变电场对脑组织突触可塑性的调节。","authors":"Chi Zhang, Yiqiang Li, Li Yang, Hongwei Zhao","doi":"10.1007/s13534-023-00287-7","DOIUrl":null,"url":null,"abstract":"Purpose: External electric fields can regulate the neural network and change the excitability of the in-vivo cerebral cortex. Here, to prove the effect of alternating electric fields on the synaptic plasticity of ex-vivo tissues, the regular changes in the synaptic structure under alternating electric fields were studied.Methods: This study applied alternating electric fields with a peak voltage of 20 V and frequencies of 5, 20, 50, and 80 Hz to the porcine cerebral cortex. Relying on transmission electron microscopy (TEM), the ultrastructure of synapses was observed, and the curvature radius of post-synaptic density (PSD) and the synaptic gap distance was quantified.Results: The results indicated that under alternating electric fields, the average synaptic curvature of the PSD decreased by 30-59% with increasing frequency, and the average synaptic gap distance became narrower.Conclusion: In ex-vivo brain tissue, synaptic plasticity can be regulated by alternating electric fields of different frequencies. This study can provide reference data for the storage and regulation of ex-vivo organs, as well as comparable data for in-vivo studies.","PeriodicalId":46898,"journal":{"name":"Biomedical Engineering Letters","volume":"13 3","pages":"391-396"},"PeriodicalIF":3.2000,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382455/pdf/","citationCount":"0","resultStr":"{\"title\":\"Regulation of local alternating electric fields on synaptic plasticity in brain tissue.\",\"authors\":\"Chi Zhang, Yiqiang Li, Li Yang, Hongwei Zhao\",\"doi\":\"10.1007/s13534-023-00287-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Purpose: External electric fields can regulate the neural network and change the excitability of the in-vivo cerebral cortex. Here, to prove the effect of alternating electric fields on the synaptic plasticity of ex-vivo tissues, the regular changes in the synaptic structure under alternating electric fields were studied.Methods: This study applied alternating electric fields with a peak voltage of 20 V and frequencies of 5, 20, 50, and 80 Hz to the porcine cerebral cortex. Relying on transmission electron microscopy (TEM), the ultrastructure of synapses was observed, and the curvature radius of post-synaptic density (PSD) and the synaptic gap distance was quantified.Results: The results indicated that under alternating electric fields, the average synaptic curvature of the PSD decreased by 30-59% with increasing frequency, and the average synaptic gap distance became narrower.Conclusion: In ex-vivo brain tissue, synaptic plasticity can be regulated by alternating electric fields of different frequencies. This study can provide reference data for the storage and regulation of ex-vivo organs, as well as comparable data for in-vivo studies.\",\"PeriodicalId\":46898,\"journal\":{\"name\":\"Biomedical Engineering Letters\",\"volume\":\"13 3\",\"pages\":\"391-396\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2023-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382455/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical Engineering Letters\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s13534-023-00287-7\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/8/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Engineering Letters","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s13534-023-00287-7","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/8/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}

引用次数: 0

摘要

目的：外部电场可以调节神经网络，改变体内大脑皮层的兴奋性。为了证明交变电场对离体组织突触可塑性的影响，研究了交变电场下突触结构的规律变化。方法：本研究在猪大脑皮层施加峰值电压为20V、频率为5、20、50和80Hz的交变电场。利用透射电子显微镜（TEM）观察突触的超微结构，定量测定突触后密度（PSD）的曲率半径和突触间隙距离。结果：在交变电场下，PSD的平均突触曲率随着频率的增加而减小30-59%，平均突触间隙距离变窄。结论：在离体脑组织中，不同频率的交变电场可以调节突触可塑性。该研究可为离体器官的储存和调节提供参考数据，也可为体内研究提供可比数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Regulation of local alternating electric fields on synaptic plasticity in brain tissue.

Purpose: External electric fields can regulate the neural network and change the excitability of the in-vivo cerebral cortex. Here, to prove the effect of alternating electric fields on the synaptic plasticity of ex-vivo tissues, the regular changes in the synaptic structure under alternating electric fields were studied.

Methods: This study applied alternating electric fields with a peak voltage of 20 V and frequencies of 5, 20, 50, and 80 Hz to the porcine cerebral cortex. Relying on transmission electron microscopy (TEM), the ultrastructure of synapses was observed, and the curvature radius of post-synaptic density (PSD) and the synaptic gap distance was quantified.

Results: The results indicated that under alternating electric fields, the average synaptic curvature of the PSD decreased by 30-59% with increasing frequency, and the average synaptic gap distance became narrower.

Conclusion: In ex-vivo brain tissue, synaptic plasticity can be regulated by alternating electric fields of different frequencies. This study can provide reference data for the storage and regulation of ex-vivo organs, as well as comparable data for in-vivo studies.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biomedical Engineering Letters ENGINEERING, BIOMEDICAL-

CiteScore

6.80

自引率

0.00%

发文量

期刊介绍： Biomedical Engineering Letters (BMEL) aims to present the innovative experimental science and technological development in the biomedical field as well as clinical application of new development. The article must contain original biomedical engineering content, defined as development, theoretical analysis, and evaluation/validation of a new technique. BMEL publishes the following types of papers: original articles, review articles, editorials, and letters to the editor. All the papers are reviewed in single-blind fashion.