薄层色谱-质谱-生物自显像鉴定抗酪氨酸酶化合物及从rsm优化的芦花茎醇提物的生物活性部分制备外用凝胶制剂。

IF 1.7 4区 农林科学 Q3 CHEMISTRY, ANALYTICAL
Areeba Insaf, Rabea Parveen, Varsha Srivastava, Monalisha Samal, Muzayyana Khan, Sayeed Ahmad
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引用次数: 0

摘要

背景:茜草科茜草在全球范围内被报道可以治疗皮肤相关问题。本研究旨在评估茜草(ARC)抗酪氨酸酶的潜力和凝胶制剂的开发。方法:使用AutoDock Vina(版本V.1.2.0)程序包进行分子对接,以检查配体与蛋白质的结合亲和力。采用响应面法(RSM)软件对茜草醇提物的提取参数进行了优化。根据国际协调会议(ICH)指南,对开发的用于定量ARC中嘌呤的HPTLC方法进行了验证。进行了一项评估抗酪氨酸酶作用的生物图谱研究;开发并评价了一种可用于减少皮肤色素沉着的富含蒽醌部分(AEF)的凝胶制剂。结果:嘌呤表现出最佳结合亲和力(-7.4 kcal/mol)与分子靶标(酪氨酸酶)的结合,与标准曲酸的结合(-5.3 kcal/mol)。通过RSM软件优化的ARC中嘌呤的定量得到了验证,并观察到AEF的抗酪氨酸酶潜力的生理学显著结果,同时对抗酪氨酸酶化合物:嘌呤(m/z256.21)和鞣花酸(m/z302.19)进行了TLC-MS生物图谱鉴定。通过体外和离体渗透研究对AEF凝胶制剂进行了评价。结论:RSM优化ARC提取参数,并进行生物图谱研究,有助于鉴定抗酪氨酸酶化合物。开发凝胶制剂可能是未来治疗色素脱失的一种具有成本效益的选择。亮点:基于薄层色谱-质谱生物图谱的抗酪氨酸酶化合物鉴定,以及从茜草茎的RSM优化醇提取物的生物活性部分开发AEF负载的局部凝胶制剂,这可能有助于减少皮肤色素沉着和保持均匀色调。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
TLC-MS-Bioautographic Identification of Antityrosinase Compounds and Preparation of a Topical Gel Formulation from a Bioactive Fraction of an RSM-Optimized Alcoholic Extract of Rubia Cordifolia L. stem.

Background: Rubia cordifolia L., Rubiaceae, is globally reported to treat skin-related problems. The study aimed to assess the antityrosinase potential of Rubia cordifolia (ARC) and the development of gel formulation.

Methods: The AutoDock Vina (version V.1.2.0) program package was used for molecular docking to check for the binding affinity of ligands with protein. Response surface methodology (RSM) software was used to optimize extraction parameters for an alcoholic extract of Rubia cordifolia (ARC). The developed HPTLC method for the quantification of purpurin in ARC was validated as per the International Conference on Harmonization (ICH) guidelines. A bioautographic study for the evaluation of antityrosinase effects was performed; an anthraquinone-enriched fraction (AEF)-loaded gel formulation developed and evaluated physicochemically which could be used to reduce skin pigmentation.

Results: Purpurin showed optimum binding affinity (-7.4 kcal/mol) with the molecular target (tyrosinase) when compared to that of standard kojic acid (-5.3 kcal/mol). Quantification of purpurin in ARC, optimized by RSM software, was validated and physiologically significant results were observed for the antityrosinase potential of an AEF, along with TLC-MS-bioautographic identification for antityrosinase compounds: purpurin (m/z 256.21) and ellagic acid (m/z 302.19). Evaluation of an AEF-loaded gel formulation by in vitro and ex vivo permeation studies was performed.

Conclusion: ARC extraction parameters optimized by RSM, and a bioautographic study helped identify antityrosinase compounds. The development of a gel formulation could be a cost-effective option for the treatment of depigmentation in the future.

Highlights: A TLC-MS-Bioautography-based Identification of Antityrosinase Compounds and development of AEF-loaded Topical Gel formulation from a Bioactive Fraction of an RSM-Optimized Alcoholic Extract of Rubia Cordifolia L. stem, which could help with promising results in reducing skin pigmentation and maintaining even tone.

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来源期刊
Journal of AOAC International
Journal of AOAC International 医学-分析化学
CiteScore
3.10
自引率
12.50%
发文量
144
审稿时长
2.7 months
期刊介绍: The Journal of AOAC INTERNATIONAL publishes the latest in basic and applied research in analytical sciences related to foods, drugs, agriculture, the environment, and more. The Journal is the method researchers'' forum for exchanging information and keeping informed of new technology and techniques pertinent to regulatory agencies and regulated industries.
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