KangarooSci®好氧计数板在选定食品和不锈钢环境表面中嗜氧需氧细菌计数的验证:AOAC性能测试方法sm 062301。

IF 1.7 4区 农林科学 Q3 CHEMISTRY, ANALYTICAL
Danping Wei, Di Wu, Jisheng Wang, Yazhou Li, Xinyu Sun, Can Zhang, Shuai Zhang, Wesley Thompson, M Joseph Benzinger, Erin Crowley
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引用次数: 0

摘要

背景:KangaroSci®需氧菌计数板(ACP)是一种可直接计数48-72后需氧菌菌落的样品培养基系统 孵育h。目的:对KangaroSci-ACP进行AOAC性能测试方法SM认证。方法:通过基质研究、产品一致性/稳定性研究和稳健性测试对KangaroSci-ACP进行评估。在基质研究中,九种食品(脱脂奶粉、新鲜生牛乳、巴氏灭菌液态牛乳、新鲜生碎牛肉、冷冻生鸡胸肉、熟肉丝、苹果汁、冰淇淋和新鲜草莓)和一种环境表面(不锈钢)按照KangaroSci-ACP产品说明进行了评估,并与ISO 4833-1:2013进行了比较,食品和动物饲料的微生物学微生物计数的水平方法第1部分:30时的菌落计数 °C,采用浇注板技术参考标准。产品一致性和稳定性测试评估了KangaroSci-ACP的三个独立生产批次。稳健性测试使用析因研究设计检查了三个测试参数,即样品电镀体积、培养时间和培养温度。结果:基质研究的结果表明,KangaroSci-ACP和ISO 4833-1:2013参考标准的性能相当。产品一致性和稳定性测试表明,在长达12 月和生产批次之间。在稳健性测试期间,操作测试条件的微小变化对性能没有显著影响。结论:KangaroSci-ACP是一种对所有基质进行有氧平板计数的有效方法。亮点:袋鼠科学ACP允许快速、可靠地计数需氧细菌。与参考方法相比,使用替代方法在培养箱中占用更少的空间,不需要样品散布器,并且需要更少的耗材。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Validation of the KangarooSci® Aerobic Count Plate for the Enumeration of Meosphilic Aerobic Bacteria in Selected Foods and on Stainless Steel Environmental Surfaces: AOAC Performance Tested MethodSM 062301.

Background: The KangarooSci® Aerobic Count Plate (ACP) is a sample-ready culture medium system for direct counting of aerobic bacteria colonies after 48-72 h of incubation.

Objective: The KangarooSci ACP was evaluated for AOAC Performance Tested MethodsSM certification.

Methods: The KangarooSci ACP was evaluated through matrix studies and product consistency/stability study and robustness testing. For the matrix study, nine food products (nonfat dry milk powder, fresh raw bovine milk, pasteurized liquid bovine milk, fresh raw ground beef, frozen uncooked chicken breast, cooked shredded pork, apple juice, ice cream, and fresh strawberries), and one environmental surface (stainless steel) were evaluated following the KangarooSci ACP product instructions and compared to the ISO 4833-1:2013, Microbiology of food and animal feeding stuffs-Horizontal methods for the enumeration of microorganisms-Part 1: Colony count at 30 °C by the pour plate technique reference standard. The product consistency and stability testing evaluated three separate production lots of the KangarooSci ACP. The robustness testing examined three test parameters, volume of sample plated, incubation time, and incubation temperature, using a factorial study design.

Results: Results from the matrix study demonstrated equivalent performance between the KangarooSci ACP and the ISO 4833-1:2013 reference standard. The product consistency and stability testing showed that the performance of the assay was equivalent over time up to 12 months and between production lots. Minor changes to the operational test conditions showed no significant impact on performance during the robustness testing.

Conclusion: The KangarooSci ACP is an effective method for aerobic plate count for all matrixes evaluated.

Highlights: The KangarooSci ACP allows for fast, reliable enumeration of aerobic bacteria. Utilizing the alternative method takes up less space in incubators, requires no sample spreader, and requires fewer consumables compared to the reference method.

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来源期刊
Journal of AOAC International
Journal of AOAC International 医学-分析化学
CiteScore
3.10
自引率
12.50%
发文量
144
审稿时长
2.7 months
期刊介绍: The Journal of AOAC INTERNATIONAL publishes the latest in basic and applied research in analytical sciences related to foods, drugs, agriculture, the environment, and more. The Journal is the method researchers'' forum for exchanging information and keeping informed of new technology and techniques pertinent to regulatory agencies and regulated industries.
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