Gang Chen , Salma Mostafa , Zhaogeng Lu , Ran Du , Jiawen Cui , Yun Wang , Qinggang Liao , Jinkai Lu , Xinyu Mao , Bang Chang , Quan Gan , Li Wang , Zhichao Jia , Xiulian Yang , Yingfang Zhu , Jianbin Yan , Biao Jin
{"title":"茉莉花（Jasminum sambac）基因组深入了解花香和茉莉酸盐的生物合成","authors":"Gang Chen , Salma Mostafa , Zhaogeng Lu , Ran Du , Jiawen Cui , Yun Wang , Qinggang Liao , Jinkai Lu , Xinyu Mao , Bang Chang , Quan Gan , Li Wang , Zhichao Jia , Xiulian Yang , Yingfang Zhu , Jianbin Yan , Biao Jin","doi":"10.1016/j.gpb.2022.12.005","DOIUrl":null,"url":null,"abstract":"<div><p><strong><em>Jasminum sambac</em></strong> (<strong>jasmine flower</strong>), a world-renowned plant appreciated for its exceptional <strong>flower fragrance</strong>, is of cultural and economic importance. However, the genetic basis of its fragrance is largely unknown. Here, we present the first <em>de novo</em> <strong>genome</strong> assembly of <em>J. sambac</em> with 550.12 Mb (scaffold N50 = 40.10 Mb) assembled into 13 pseudochromosomes. Terpene synthase (TPS) genes associated with flower fragrance are considerably amplified in the form of gene clusters through tandem duplications in the genome. Gene clusters within the salicylic acid/benzoic acid/theobromine (SABATH) and benzylalcohol <em>O</em>-acetyltransferase/anthocyanin <em>O</em>-hydroxycinnamoyltransferases/anthranilate <em>N</em>-hydroxycinnamoyl/benzoyltransferase/deacetylvindoline 4-<em>O</em>-acetyltransferase (BAHD) superfamilies were identified to be related to the biosynthesis of phenylpropanoid/benzenoid compounds. Several key genes involved in <strong>jasmonate</strong> biosynthesis were duplicated, causing an increase in copy numbers. In addition, multi-omics analyses identified various aromatic compounds and many genes involved in fragrance biosynthesis pathways. Furthermore, the roles of <em>JsTPS3</em> in β-ocimene biosynthesis, as well as <em>JsAOC1</em> and <em>JsAOS</em> in jasmonic acid biosynthesis, were functionally validated. The genome assembled in this study for <em>J. sambac</em> offers a basic genetic resource for studying floral scent and jasmonate biosynthesis, and provides a foundation for functional genomic research and variety improvements in <em>Jasminum</em>.</p></div>","PeriodicalId":12528,"journal":{"name":"Genomics, Proteomics & Bioinformatics","volume":"21 1","pages":"Pages 127-149"},"PeriodicalIF":11.5000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10372924/pdf/","citationCount":"5","resultStr":"{\"title\":\"The Jasmine (Jasminum sambac) Genome Provides Insight into the Biosynthesis of Flower Fragrances and Jasmonates\",\"authors\":\"Gang Chen , Salma Mostafa , Zhaogeng Lu , Ran Du , Jiawen Cui , Yun Wang , Qinggang Liao , Jinkai Lu , Xinyu Mao , Bang Chang , Quan Gan , Li Wang , Zhichao Jia , Xiulian Yang , Yingfang Zhu , Jianbin Yan , Biao Jin\",\"doi\":\"10.1016/j.gpb.2022.12.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><strong><em>Jasminum sambac</em></strong> (<strong>jasmine flower</strong>), a world-renowned plant appreciated for its exceptional <strong>flower fragrance</strong>, is of cultural and economic importance. However, the genetic basis of its fragrance is largely unknown. Here, we present the first <em>de novo</em> <strong>genome</strong> assembly of <em>J. sambac</em> with 550.12 Mb (scaffold N50 = 40.10 Mb) assembled into 13 pseudochromosomes. Terpene synthase (TPS) genes associated with flower fragrance are considerably amplified in the form of gene clusters through tandem duplications in the genome. Gene clusters within the salicylic acid/benzoic acid/theobromine (SABATH) and benzylalcohol <em>O</em>-acetyltransferase/anthocyanin <em>O</em>-hydroxycinnamoyltransferases/anthranilate <em>N</em>-hydroxycinnamoyl/benzoyltransferase/deacetylvindoline 4-<em>O</em>-acetyltransferase (BAHD) superfamilies were identified to be related to the biosynthesis of phenylpropanoid/benzenoid compounds. Several key genes involved in <strong>jasmonate</strong> biosynthesis were duplicated, causing an increase in copy numbers. In addition, multi-omics analyses identified various aromatic compounds and many genes involved in fragrance biosynthesis pathways. Furthermore, the roles of <em>JsTPS3</em> in β-ocimene biosynthesis, as well as <em>JsAOC1</em> and <em>JsAOS</em> in jasmonic acid biosynthesis, were functionally validated. The genome assembled in this study for <em>J. sambac</em> offers a basic genetic resource for studying floral scent and jasmonate biosynthesis, and provides a foundation for functional genomic research and variety improvements in <em>Jasminum</em>.</p></div>\",\"PeriodicalId\":12528,\"journal\":{\"name\":\"Genomics, Proteomics & Bioinformatics\",\"volume\":\"21 1\",\"pages\":\"Pages 127-149\"},\"PeriodicalIF\":11.5000,\"publicationDate\":\"2023-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10372924/pdf/\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genomics, Proteomics & Bioinformatics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1672022922001711\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genomics, Proteomics & Bioinformatics","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1672022922001711","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
The Jasmine (Jasminum sambac) Genome Provides Insight into the Biosynthesis of Flower Fragrances and Jasmonates
Jasminum sambac (jasmine flower), a world-renowned plant appreciated for its exceptional flower fragrance, is of cultural and economic importance. However, the genetic basis of its fragrance is largely unknown. Here, we present the first de novogenome assembly of J. sambac with 550.12 Mb (scaffold N50 = 40.10 Mb) assembled into 13 pseudochromosomes. Terpene synthase (TPS) genes associated with flower fragrance are considerably amplified in the form of gene clusters through tandem duplications in the genome. Gene clusters within the salicylic acid/benzoic acid/theobromine (SABATH) and benzylalcohol O-acetyltransferase/anthocyanin O-hydroxycinnamoyltransferases/anthranilate N-hydroxycinnamoyl/benzoyltransferase/deacetylvindoline 4-O-acetyltransferase (BAHD) superfamilies were identified to be related to the biosynthesis of phenylpropanoid/benzenoid compounds. Several key genes involved in jasmonate biosynthesis were duplicated, causing an increase in copy numbers. In addition, multi-omics analyses identified various aromatic compounds and many genes involved in fragrance biosynthesis pathways. Furthermore, the roles of JsTPS3 in β-ocimene biosynthesis, as well as JsAOC1 and JsAOS in jasmonic acid biosynthesis, were functionally validated. The genome assembled in this study for J. sambac offers a basic genetic resource for studying floral scent and jasmonate biosynthesis, and provides a foundation for functional genomic research and variety improvements in Jasminum.
期刊介绍：
Genomics, Proteomics and Bioinformatics (GPB) is the official journal of the Beijing Institute of Genomics, Chinese Academy of Sciences / China National Center for Bioinformation and Genetics Society of China. It aims to disseminate new developments in the field of omics and bioinformatics, publish high-quality discoveries quickly, and promote open access and online publication. GPB welcomes submissions in all areas of life science, biology, and biomedicine, with a focus on large data acquisition, analysis, and curation. Manuscripts covering omics and related bioinformatics topics are particularly encouraged. GPB is indexed/abstracted by PubMed/MEDLINE, PubMed Central, Scopus, BIOSIS Previews, Chemical Abstracts, CSCD, among others.
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