Claudin-3对慢性鼻窦炎伴鼻息肉患者上皮屏障功能障碍的预测意义。

IF 4.1 2区 医学 Q2 ALLERGY
Zhi-Qun Huang, Jing Ye, Jing Liu, Li-Ying Sun, Hsiao Hui Ong, Yong-Hao Wei, Shu-Cai Fu, Xiao-Xun Hu, Yu Xu, De-Yun Wang
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引用次数: 1

摘要

目的:紧密连接蛋白(tight junction, TJ)的异常表达在慢性鼻窦炎伴鼻息肉(CRSwNP)的发病过程中起着至关重要的作用。然而,在临床实践中,没有合适的工具来区分和诊断上皮屏障缺陷。本研究旨在评估claudin-3对CRSwNP中上皮屏障功能障碍的预测价值。方法:本研究采用实时定量聚合酶链反应、免疫荧光和免疫组织化学染色检测对照组和CRSwNP患者的TJ蛋白水平。建立受试者工作特征(ROC)曲线,评价TJ分解对临床预后的预测价值。体外培养人鼻上皮细胞,测定其经皮电阻(TER)水平。结果:CRSwNP患者occludin、tricellulin、claudin-3、claudin-10的表达水平较健康者降低(P < 0.05), claudin-1表达水平较健康者升高(P < 0.05)。此外,claudin-3和occludin水平与CRSwNP的计算机断层扫描评分呈负相关(均P < 0.05), ROC曲线显示claudin-3水平在评估上皮屏障破坏方面具有最高的预测准确性(曲线下面积= 0.791,P < 0.001)。最后,时间序列分析显示,TER与claudin-3的相关系数最高(互相关函数= 0.75)。结论:在本研究中,我们提示cludin -3可能是预测CRSwNP患者鼻上皮屏障缺陷和疾病严重程度的有价值的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Predictive Significance of Claudin-3 for Epithelial Barrier Dysfunction in Chronic Rhinosinusitis With Nasal Polyps.

Predictive Significance of Claudin-3 for Epithelial Barrier Dysfunction in Chronic Rhinosinusitis With Nasal Polyps.

Predictive Significance of Claudin-3 for Epithelial Barrier Dysfunction in Chronic Rhinosinusitis With Nasal Polyps.

Predictive Significance of Claudin-3 for Epithelial Barrier Dysfunction in Chronic Rhinosinusitis With Nasal Polyps.

Purpose: The abnormal expression of tight junction (TJ) plays a vital role in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). However, there is no appropriate tool to distinguish and diagnose epithelial barrier defects in clinical practice. This study aimed to evaluate the predictive value of claudin-3 for epithelial barrier dysfunction in CRSwNP.

Methods: In this study, TJ protein levels were evaluated by real-time quantitative polymerase chain reaction, immunofluorescent, and immunohistochemistry staining in control subjects and CRSwNP patients. The receiver operating characteristic (ROC) curve was created to assess the predictive value of TJ breakdown in clinical outcomes. In vitro, human nasal epithelial cells were cultured at the air-liquid interface to analyze the transepithelial electrical resistance (TER) level.

Results: The expression levels of occludin, tricellulin, claudin-3, and claudin-10 were decreased (all P < 0.05), and those of claudin-1 was increased (P < 0.05) in CRSwNP patients as compared to healthy subjects. Additionally, claudin-3 and occludin levels were negatively correlated with the computed tomography score in CRSwNP (all P < 0.05), and the ROC curve indicated that the claudin-3 level had the most predictive accuracy in evaluating epithelial barrier disruption (area under the curve = 0.791, P < 0.001). Finally, the time-series analysis showed the highest correlation coefficient between TER and claudin-3 (cross-correlation function = 0.75).

Conclusion: In this study, we suggest that claudin-3 could be a valuable biomarker for predicting nasal epithelial barrier defects and disease severity in CRSwNP.

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来源期刊
CiteScore
6.10
自引率
6.80%
发文量
53
审稿时长
>12 weeks
期刊介绍: The journal features cutting-edge original research, brief communications, and state-of-the-art reviews in the specialties of allergy, asthma, and immunology, including clinical and experimental studies and instructive case reports. Contemporary reviews summarize information on topics for researchers and physicians in the fields of allergy and immunology. As of January 2017, AAIR do not accept case reports. However, if it is a clinically important case, authors can submit it in the form of letter to the Editor. Editorials and letters to the Editor explore controversial issues and encourage further discussion among physicians dealing with allergy, immunology, pediatric respirology, and related medical fields. AAIR also features topics in practice and management and recent advances in equipment and techniques for clinicians concerned with clinical manifestations of allergies and pediatric respiratory diseases.
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