{"title":"常规根管冲洗剂和壳聚糖纳米粒子抗真菌效果的比较评价。","authors":"Omkar Balsaraf, Srinidhi Surya Raghavendra, Dipali Shah, Mulay Sanjyot, Aishwarya Balsaraf","doi":"10.4103/jcd.jcd_617_22","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>The purpose of this study was to evaluate the efficacy of 3% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and chitosan nanoparticles against <i>Candida albicans</i> using the agar disc-diffusion test.</p><p><strong>Materials and methods: </strong>Strain of <i>C. albicans</i> was cultivated in Sabouraud Dextrose Agar. Chitosan nanoparticles were synthesized using an ionic gelation method. Four groups were made according to the irrigants used. Group 1: 3% NaOCl, Group 2: 2% CHX, Group 3: chitosan nanoparticles, and Group 4: saline as control. Discs were added with the different irrigants and placed in a dish containing <i>C. albicans</i>. The plates were incubated at 37°C for 24h. The zone of inhibition was measured in millimeter.</p><p><strong>Results: </strong>Statistical analysis was performed using the test of one-way variance (ANOVA) and <i>post hoc</i> Tukey. Group 1 showed significantly higher zone of inhibition compared to Groups 2 and 3 (<i>P</i> < 0.05). There were no significant differences between the zones of inhibition of Groups 2 and 3 (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>Chitosan nanoparticles and 2% CHX have similar efficacy against <i>C. albicans</i>, whereas 3% NaOCl was significantly better than both chitosan nanoparticles and CHX.</p>","PeriodicalId":38892,"journal":{"name":"Journal of Conservative Dentistry","volume":"26 2","pages":"226-229"},"PeriodicalIF":0.0000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10190093/pdf/","citationCount":"0","resultStr":"{\"title\":\"Comparative evaluation of antifungal efficacy of conventional endodontic irrigants and chitosan nanoparticles.\",\"authors\":\"Omkar Balsaraf, Srinidhi Surya Raghavendra, Dipali Shah, Mulay Sanjyot, Aishwarya Balsaraf\",\"doi\":\"10.4103/jcd.jcd_617_22\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>The purpose of this study was to evaluate the efficacy of 3% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and chitosan nanoparticles against <i>Candida albicans</i> using the agar disc-diffusion test.</p><p><strong>Materials and methods: </strong>Strain of <i>C. albicans</i> was cultivated in Sabouraud Dextrose Agar. Chitosan nanoparticles were synthesized using an ionic gelation method. Four groups were made according to the irrigants used. Group 1: 3% NaOCl, Group 2: 2% CHX, Group 3: chitosan nanoparticles, and Group 4: saline as control. Discs were added with the different irrigants and placed in a dish containing <i>C. albicans</i>. The plates were incubated at 37°C for 24h. The zone of inhibition was measured in millimeter.</p><p><strong>Results: </strong>Statistical analysis was performed using the test of one-way variance (ANOVA) and <i>post hoc</i> Tukey. Group 1 showed significantly higher zone of inhibition compared to Groups 2 and 3 (<i>P</i> < 0.05). There were no significant differences between the zones of inhibition of Groups 2 and 3 (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>Chitosan nanoparticles and 2% CHX have similar efficacy against <i>C. albicans</i>, whereas 3% NaOCl was significantly better than both chitosan nanoparticles and CHX.</p>\",\"PeriodicalId\":38892,\"journal\":{\"name\":\"Journal of Conservative Dentistry\",\"volume\":\"26 2\",\"pages\":\"226-229\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10190093/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Conservative Dentistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/jcd.jcd_617_22\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/3/16 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"Dentistry\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Conservative Dentistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jcd.jcd_617_22","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/3/16 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"Dentistry","Score":null,"Total":0}
Comparative evaluation of antifungal efficacy of conventional endodontic irrigants and chitosan nanoparticles.
Aim: The purpose of this study was to evaluate the efficacy of 3% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and chitosan nanoparticles against Candida albicans using the agar disc-diffusion test.
Materials and methods: Strain of C. albicans was cultivated in Sabouraud Dextrose Agar. Chitosan nanoparticles were synthesized using an ionic gelation method. Four groups were made according to the irrigants used. Group 1: 3% NaOCl, Group 2: 2% CHX, Group 3: chitosan nanoparticles, and Group 4: saline as control. Discs were added with the different irrigants and placed in a dish containing C. albicans. The plates were incubated at 37°C for 24h. The zone of inhibition was measured in millimeter.
Results: Statistical analysis was performed using the test of one-way variance (ANOVA) and post hoc Tukey. Group 1 showed significantly higher zone of inhibition compared to Groups 2 and 3 (P < 0.05). There were no significant differences between the zones of inhibition of Groups 2 and 3 (P < 0.05).
Conclusion: Chitosan nanoparticles and 2% CHX have similar efficacy against C. albicans, whereas 3% NaOCl was significantly better than both chitosan nanoparticles and CHX.
期刊介绍：
The Journal of Conservative Dentistry (ISSN - 0972-0707) is the official journal of the Indian Association of Conservative Dentistry and Endodontics (IACDE). Our journal publishes scientific articles, case reports, short communications, invited reviews and comparative studies evaluating materials and methods in the fields of Conservative Dentistry, Dental Materials and Endodontics. J Conserv Dent has a diverse readership that includes full-time clinicians, full-time academicians, residents, students and scientists. Effective communication with this diverse readership requires careful attention to writing style.
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