Wafaa Achache, Jean Louis Mege, Mustapha Fellag, Michel Drancourt
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引用次数: 0
摘要
肠球菌(一种肠道共生菌)已被证实能抑制某些导致人类和哺乳动物结核病的结核分枝杆菌复合体(MTC)菌株的生长。为了进一步探究这一初步观察结果,我们使用标准化定量琼脂井扩散试验对五株 E. mundtii 菌株和七株 MTC 菌株进行了交叉调查,这七株菌株分别代表了四种 MTC 菌株。标定为 10 MacFarland 的所有五株 E. mundtii 菌株都抑制了具有不同敏感性的所有 M. tuberculosis 菌株的生长,但在较低的接种量下未观察到抑制作用。此外,8 种 E. mundtii 冻干无细胞培养上清液(CFCS)抑制了结核杆菌、非洲分枝杆菌、牛分枝杆菌和卡内提分枝杆菌(最易感的 MTC 菌种)的生长(抑制直径为 25±1 毫米),抑制作用与 CFCS 蛋白浓度成正比。本文报告的数据表明,E. mundtii 分泌组抑制了所有医学上感兴趣的 MTC 物种的生长,这扩大了之前报告的数据范围。在肠道中,E. mundtii分泌物可能会调节结核病的表达,表现出抗结核作用,对人类和动物健康具有一定的保护作用。
The Enterococcus secretome inhibits the growth of Mycobacterium tuberculosis complex mycobacteria.
Enterococcus mundtii , a commensal intestinal bacterium, was demonstrated to inhibit the growth of some Mycobacterium tuberculosis complex (MTC) species that cause tuberculosis in humans and mammals. To further explore this preliminary observation, we cross-investigated five E. mundtii strains and seven MTC strains representative of four MTC species using a standardized quantitative agar well diffusion assay. All five E. mundtii strains, calibrated at 10 MacFarland, inhibited the growth of all M. tuberculosis strains with various susceptibility profiles, but no inhibition was observed with lower inoculums. Further, eight E. mundtii freeze-dried cell-free culture supernatants (CFCS) inhibited the growth of M. tuberculosis , Mycobacterium africanum, Mycobacterium bovis and Mycobacterium canettii, the most susceptible MTC species (inhibition diameter 25±1 mm), proportionally to CFCS protein concentrations. The data reported here indicate that the E. mundtii secretome inhibited growth of all MTC species of medical interest, which broadens previously reported data. In the gut, the E. mundtii secretome may modulate the expression of tuberculosis, exhibiting an anti-tuberculosis effect, with some protective roles in human and animal health.