高度网络化的严重急性呼吸系统综合征冠状病毒2型肽以增强的特异性激发T细胞反应。

Gabriel Duette, Eunok Lee, Gabriela Martins Costa Gomes, Katie Tungatt, Chloe Doyle, Vicki V Stylianou, Ashley Lee, Susan Maddocks, Janette Taylor, Rajiv Khanna, Rowena A Bull, Marianne Martinello, Kerrie J Sandgren, Anthony L Cunningham, Sarah Palmer
{"title":"高度网络化的严重急性呼吸系统综合征冠状病毒2型肽以增强的特异性激发T细胞反应。","authors":"Gabriel Duette, Eunok Lee, Gabriela Martins Costa Gomes, Katie Tungatt, Chloe Doyle, Vicki V Stylianou, Ashley Lee, Susan Maddocks, Janette Taylor, Rajiv Khanna, Rowena A Bull, Marianne Martinello, Kerrie J Sandgren, Anthony L Cunningham, Sarah Palmer","doi":"10.4049/immunohorizons.2300034","DOIUrl":null,"url":null,"abstract":"<p><p>Identifying SARS-CoV-2-specific T cell epitope-derived peptides is critical for the development of effective vaccines and measuring the duration of specific SARS-CoV-2 cellular immunity. In this regard, we previously identified T cell epitope-derived peptides within topologically and structurally essential regions of SARS-CoV-2 spike and nucleocapsid proteins by applying an immunoinformatics pipeline. In this study, we selected 30 spike- and nucleocapsid-derived peptides and assessed whether these peptides induce T cell responses and avoid major mutations found in SARS-CoV-2 variants of concern. Our peptide pool was highly specific, with only a single peptide driving cross-reactivity in people unexposed to SARS-COV-2, and immunogenic, inducing a polyfunctional response in CD4+ and CD8+ T cells from COVID-19 recovered individuals. All peptides were immunogenic and individuals recognized broad and diverse peptide repertoires. Moreover, our peptides avoided most mutations/deletions associated with all four SARS-CoV-2 variants of concern while retaining their physicochemical properties even when genetic changes are introduced. This study contributes to an evolving definition of individual CD4+ and CD8+ T cell epitopes that can be used for specific diagnostic tools for SARS-CoV-2 T cell responses and is relevant to the development of variant-resistant and durable T cell-stimulating vaccines.</p>","PeriodicalId":13448,"journal":{"name":"ImmunoHorizons","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5a/dc/ih2300034.PMC10580120.pdf","citationCount":"0","resultStr":"{\"title\":\"Highly Networked SARS-CoV-2 Peptides Elicit T Cell Responses with Enhanced Specificity.\",\"authors\":\"Gabriel Duette, Eunok Lee, Gabriela Martins Costa Gomes, Katie Tungatt, Chloe Doyle, Vicki V Stylianou, Ashley Lee, Susan Maddocks, Janette Taylor, Rajiv Khanna, Rowena A Bull, Marianne Martinello, Kerrie J Sandgren, Anthony L Cunningham, Sarah Palmer\",\"doi\":\"10.4049/immunohorizons.2300034\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Identifying SARS-CoV-2-specific T cell epitope-derived peptides is critical for the development of effective vaccines and measuring the duration of specific SARS-CoV-2 cellular immunity. In this regard, we previously identified T cell epitope-derived peptides within topologically and structurally essential regions of SARS-CoV-2 spike and nucleocapsid proteins by applying an immunoinformatics pipeline. In this study, we selected 30 spike- and nucleocapsid-derived peptides and assessed whether these peptides induce T cell responses and avoid major mutations found in SARS-CoV-2 variants of concern. Our peptide pool was highly specific, with only a single peptide driving cross-reactivity in people unexposed to SARS-COV-2, and immunogenic, inducing a polyfunctional response in CD4+ and CD8+ T cells from COVID-19 recovered individuals. All peptides were immunogenic and individuals recognized broad and diverse peptide repertoires. Moreover, our peptides avoided most mutations/deletions associated with all four SARS-CoV-2 variants of concern while retaining their physicochemical properties even when genetic changes are introduced. This study contributes to an evolving definition of individual CD4+ and CD8+ T cell epitopes that can be used for specific diagnostic tools for SARS-CoV-2 T cell responses and is relevant to the development of variant-resistant and durable T cell-stimulating vaccines.</p>\",\"PeriodicalId\":13448,\"journal\":{\"name\":\"ImmunoHorizons\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5a/dc/ih2300034.PMC10580120.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ImmunoHorizons\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4049/immunohorizons.2300034\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ImmunoHorizons","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4049/immunohorizons.2300034","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

鉴定严重急性呼吸系统综合征冠状病毒2型特异性T细胞表位衍生肽对于开发有效疫苗和测量特异性严重急性呼吸系冠状病毒2型细胞免疫的持续时间至关重要。在这方面,我们之前通过应用免疫信息学管道在严重急性呼吸系统综合征冠状病毒2型刺突蛋白和核衣壳蛋白的拓扑和结构必需区域内鉴定了T细胞表位衍生肽。在这项研究中,我们选择了30种来源于刺突和核衣壳的肽,并评估这些肽是否诱导T细胞反应并避免在SARS-CoV-2变异毒株中发现的主要突变。我们的肽库具有高度特异性,只有一种肽在未暴露于SARS-COV-2的人中驱动交叉反应,并且具有免疫原性,在新冠肺炎康复者的CD4+和CD8+T细胞中诱导多功能反应。所有肽都具有免疫原性,个体识别出广泛多样的肽库。此外,我们的肽避免了与所有四种SARS-CoV-2变异毒株相关的大多数突变/缺失,同时即使在引入遗传变化时也保留了它们的物理化学性质。这项研究有助于对单个CD4+和CD8+T细胞表位的不断发展的定义,这些表位可用于严重急性呼吸系统综合征冠状病毒2型T细胞反应的特异性诊断工具,并与开发具有变异耐药性和持久性T细胞刺激疫苗相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Highly Networked SARS-CoV-2 Peptides Elicit T Cell Responses with Enhanced Specificity.

Highly Networked SARS-CoV-2 Peptides Elicit T Cell Responses with Enhanced Specificity.

Highly Networked SARS-CoV-2 Peptides Elicit T Cell Responses with Enhanced Specificity.

Highly Networked SARS-CoV-2 Peptides Elicit T Cell Responses with Enhanced Specificity.

Identifying SARS-CoV-2-specific T cell epitope-derived peptides is critical for the development of effective vaccines and measuring the duration of specific SARS-CoV-2 cellular immunity. In this regard, we previously identified T cell epitope-derived peptides within topologically and structurally essential regions of SARS-CoV-2 spike and nucleocapsid proteins by applying an immunoinformatics pipeline. In this study, we selected 30 spike- and nucleocapsid-derived peptides and assessed whether these peptides induce T cell responses and avoid major mutations found in SARS-CoV-2 variants of concern. Our peptide pool was highly specific, with only a single peptide driving cross-reactivity in people unexposed to SARS-COV-2, and immunogenic, inducing a polyfunctional response in CD4+ and CD8+ T cells from COVID-19 recovered individuals. All peptides were immunogenic and individuals recognized broad and diverse peptide repertoires. Moreover, our peptides avoided most mutations/deletions associated with all four SARS-CoV-2 variants of concern while retaining their physicochemical properties even when genetic changes are introduced. This study contributes to an evolving definition of individual CD4+ and CD8+ T cell epitopes that can be used for specific diagnostic tools for SARS-CoV-2 T cell responses and is relevant to the development of variant-resistant and durable T cell-stimulating vaccines.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信