Yu-Mi Lee, Dong-Hee Choi, Jeong-Hye Park, Min-Woo Cheon, Jae Gwan Kim, Jeong-Sang Kim, Taejin Choi, Hye-Ran Kim, Daehwan Youn
{"title":"针刺对大鼠脾脏线粒体融合和分裂基因表达的影响。","authors":"Yu-Mi Lee, Dong-Hee Choi, Jeong-Hye Park, Min-Woo Cheon, Jae Gwan Kim, Jeong-Sang Kim, Taejin Choi, Hye-Ran Kim, Daehwan Youn","doi":"10.51507/j.jams.2023.16.2.49","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>A significant amount of research has been conducted to establish the validity of acupuncture, and it has been demonstrated through animal disease model studies that acupuncture influences mitochondrial changes. However, to more accurately examine the mechanisms of acupuncture treatment effectiveness in pathological models, it is crucial to investigate changes in disease-free animals. Among various hypotheses regarding the effects of acupuncture on the body, we focused on the result that acupuncture stimulation is related to mitochondria.</p><p><strong>Objectives: </strong>We examined the effects of acupuncture mitochondrial fission and fusionrelated mediators in disease-free Sprague Dawley (SD) rats' spleen meridian acupoints.</p><p><strong>Methods: </strong>SD rats were divided into control, SP1, SP2, SP3, SP5, and SP9 acupuncture groups. Acupuncture was performed at each point for 10 minutes daily for four days. Peroxisome proliferator-activated receptor-gamma coactivator 1-α (<i>PGC-1α</i>) and fission protein 1 (<i>Fis1</i>) levels were evaluated using quantitative real-time polymerase chain reaction (<i>qRT-PCR</i>), while dynamin-related protein 1 (<i>DRP1</i>), optic atrophy-1 (<i>OPA1</i>), mitofusin-1 (<i>MFN1</i>), and mitofusin-2 (<i>MFN2</i>) levels were assessed via western blotting. Mitochondria protein concentrations and NADH dehydrogenase activity in spleen tissues were measured using enzyme-linked immunosorbent assay (<i>ELISA</i>).</p><p><strong>Results: </strong>PGC-1α expression decreased in the SP1 (<i>p</i> < 0.01), SP5 (<i>p</i> < 0.05), and SP9 (<i>p</i> < 0.05) groups, while <i>Fis1</i> expression increased in the SP1 (<i>p</i> < 0.01), SP5 (<i>p</i> < 0.01), and SP9 (<i>p</i> < 0.05) groups. <i>DRP1</i>, <i>OPA1</i>, <i>MFN1</i>, and <i>MFN2</i> levels exhibited no significant changes. Mitochondrial protein concentrations decreased in the SP2 (<i>p</i> < 0.01), SP3 (<i>p</i> < 0.01), SP5 (<i>p</i> < 0.01), and SP9 (<i>p</i> < 0.01) groups, while NADH dehydrogenase activity decreased in the SP2 (<i>p</i> < 0.05) and SP9 (<i>p</i> < 0.05) groups.</p><p><strong>Conclusion: </strong>Acupuncture at the SP9 acupoint influenced the mitochondrial fission pathway by modulating <i>PGC-1α</i> and <i>Fis1</i> mediators in the rat spleen under non-disease conditions.</p>","PeriodicalId":46854,"journal":{"name":"Journal of Acupuncture and Meridian Studies","volume":"16 2","pages":"49-55"},"PeriodicalIF":0.9000,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Effects of Manual Acupuncture on Mitochondrial Fusion and Fission Gene Expression in Rat Spleen.\",\"authors\":\"Yu-Mi Lee, Dong-Hee Choi, Jeong-Hye Park, Min-Woo Cheon, Jae Gwan Kim, Jeong-Sang Kim, Taejin Choi, Hye-Ran Kim, Daehwan Youn\",\"doi\":\"10.51507/j.jams.2023.16.2.49\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>A significant amount of research has been conducted to establish the validity of acupuncture, and it has been demonstrated through animal disease model studies that acupuncture influences mitochondrial changes. However, to more accurately examine the mechanisms of acupuncture treatment effectiveness in pathological models, it is crucial to investigate changes in disease-free animals. Among various hypotheses regarding the effects of acupuncture on the body, we focused on the result that acupuncture stimulation is related to mitochondria.</p><p><strong>Objectives: </strong>We examined the effects of acupuncture mitochondrial fission and fusionrelated mediators in disease-free Sprague Dawley (SD) rats' spleen meridian acupoints.</p><p><strong>Methods: </strong>SD rats were divided into control, SP1, SP2, SP3, SP5, and SP9 acupuncture groups. Acupuncture was performed at each point for 10 minutes daily for four days. Peroxisome proliferator-activated receptor-gamma coactivator 1-α (<i>PGC-1α</i>) and fission protein 1 (<i>Fis1</i>) levels were evaluated using quantitative real-time polymerase chain reaction (<i>qRT-PCR</i>), while dynamin-related protein 1 (<i>DRP1</i>), optic atrophy-1 (<i>OPA1</i>), mitofusin-1 (<i>MFN1</i>), and mitofusin-2 (<i>MFN2</i>) levels were assessed via western blotting. Mitochondria protein concentrations and NADH dehydrogenase activity in spleen tissues were measured using enzyme-linked immunosorbent assay (<i>ELISA</i>).</p><p><strong>Results: </strong>PGC-1α expression decreased in the SP1 (<i>p</i> < 0.01), SP5 (<i>p</i> < 0.05), and SP9 (<i>p</i> < 0.05) groups, while <i>Fis1</i> expression increased in the SP1 (<i>p</i> < 0.01), SP5 (<i>p</i> < 0.01), and SP9 (<i>p</i> < 0.05) groups. <i>DRP1</i>, <i>OPA1</i>, <i>MFN1</i>, and <i>MFN2</i> levels exhibited no significant changes. Mitochondrial protein concentrations decreased in the SP2 (<i>p</i> < 0.01), SP3 (<i>p</i> < 0.01), SP5 (<i>p</i> < 0.01), and SP9 (<i>p</i> < 0.01) groups, while NADH dehydrogenase activity decreased in the SP2 (<i>p</i> < 0.05) and SP9 (<i>p</i> < 0.05) groups.</p><p><strong>Conclusion: </strong>Acupuncture at the SP9 acupoint influenced the mitochondrial fission pathway by modulating <i>PGC-1α</i> and <i>Fis1</i> mediators in the rat spleen under non-disease conditions.</p>\",\"PeriodicalId\":46854,\"journal\":{\"name\":\"Journal of Acupuncture and Meridian Studies\",\"volume\":\"16 2\",\"pages\":\"49-55\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2023-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Acupuncture and Meridian Studies\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.51507/j.jams.2023.16.2.49\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Acupuncture and Meridian Studies","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.51507/j.jams.2023.16.2.49","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
The Effects of Manual Acupuncture on Mitochondrial Fusion and Fission Gene Expression in Rat Spleen.
Background: A significant amount of research has been conducted to establish the validity of acupuncture, and it has been demonstrated through animal disease model studies that acupuncture influences mitochondrial changes. However, to more accurately examine the mechanisms of acupuncture treatment effectiveness in pathological models, it is crucial to investigate changes in disease-free animals. Among various hypotheses regarding the effects of acupuncture on the body, we focused on the result that acupuncture stimulation is related to mitochondria.
Objectives: We examined the effects of acupuncture mitochondrial fission and fusionrelated mediators in disease-free Sprague Dawley (SD) rats' spleen meridian acupoints.
Methods: SD rats were divided into control, SP1, SP2, SP3, SP5, and SP9 acupuncture groups. Acupuncture was performed at each point for 10 minutes daily for four days. Peroxisome proliferator-activated receptor-gamma coactivator 1-α (PGC-1α) and fission protein 1 (Fis1) levels were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR), while dynamin-related protein 1 (DRP1), optic atrophy-1 (OPA1), mitofusin-1 (MFN1), and mitofusin-2 (MFN2) levels were assessed via western blotting. Mitochondria protein concentrations and NADH dehydrogenase activity in spleen tissues were measured using enzyme-linked immunosorbent assay (ELISA).
Results: PGC-1α expression decreased in the SP1 (p < 0.01), SP5 (p < 0.05), and SP9 (p < 0.05) groups, while Fis1 expression increased in the SP1 (p < 0.01), SP5 (p < 0.01), and SP9 (p < 0.05) groups. DRP1, OPA1, MFN1, and MFN2 levels exhibited no significant changes. Mitochondrial protein concentrations decreased in the SP2 (p < 0.01), SP3 (p < 0.01), SP5 (p < 0.01), and SP9 (p < 0.01) groups, while NADH dehydrogenase activity decreased in the SP2 (p < 0.05) and SP9 (p < 0.05) groups.
Conclusion: Acupuncture at the SP9 acupoint influenced the mitochondrial fission pathway by modulating PGC-1α and Fis1 mediators in the rat spleen under non-disease conditions.
期刊介绍：
The Journal of Acupuncture and Meridian Studies is a quarterly, peer-reviewed journal featuring high-quality studies related to basic and clinical acupuncture and meridian research. It also includes new paradigm of integrative research, covering East–West and traditional–modern medicine. Manuscripts should fall into one of the categories: topical review, original research paper, brief ... click here for full Aims & Scope The Journal of Acupuncture and Meridian Studies is a quarterly, peer-reviewed journal featuring high-quality studies related to basic and clinical acupuncture and meridian research. It also includes new paradigm of integrative research, covering East–West and traditional–modern medicine.
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