针刺对大鼠脾脏线粒体融合和分裂基因表达的影响。

IF 0.9 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE
Yu-Mi Lee, Dong-Hee Choi, Jeong-Hye Park, Min-Woo Cheon, Jae Gwan Kim, Jeong-Sang Kim, Taejin Choi, Hye-Ran Kim, Daehwan Youn
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引用次数: 0

摘要

背景:为了建立针灸的有效性,已经进行了大量的研究,并通过动物疾病模型研究证明针灸影响线粒体的变化。然而,为了更准确地研究病理模型中针灸治疗效果的机制,研究无病动物的变化至关重要。在关于针灸对身体影响的各种假设中,我们重点关注针灸刺激与线粒体有关的结果。目的:观察针刺线粒体分裂及融合相关介质对无病大鼠脾经穴的影响。方法:将SD大鼠分为对照、SP1、SP2、SP3、SP5、SP9针刺组。每日各穴位针灸10分钟,连续4天。采用实时荧光定量聚合酶链反应(qRT-PCR)检测过氧化物酶体增殖物激活受体- γ辅助激活因子1-α (PGC-1α)和裂变蛋白1 (Fis1)水平,采用免疫印迹法检测动力蛋白相关蛋白1 (DRP1)、视神经萎缩蛋白1 (OPA1)、有丝分裂蛋白1 (MFN1)和有丝分裂蛋白2 (MFN2)水平。采用酶联免疫吸附法(ELISA)测定脾脏组织线粒体蛋白浓度和NADH脱氢酶活性。结果:PGC-1α在SP1、SP5、SP9组表达降低(p < 0.01),而Fis1在SP1、SP5、SP9组表达升高(p < 0.01)。DRP1、OPA1、MFN1、MFN2水平无明显变化。SP2、SP3、SP5、SP9组线粒体蛋白浓度降低(p < 0.01), NADH脱氢酶活性降低(p < 0.05), SP2、SP9组线粒体蛋白浓度降低(p < 0.01)。结论:针刺SP9穴通过调节脾PGC-1α和Fis1介质影响非疾病状态下大鼠脾线粒体分裂通路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effects of Manual Acupuncture on Mitochondrial Fusion and Fission Gene Expression in Rat Spleen.

Background: A significant amount of research has been conducted to establish the validity of acupuncture, and it has been demonstrated through animal disease model studies that acupuncture influences mitochondrial changes. However, to more accurately examine the mechanisms of acupuncture treatment effectiveness in pathological models, it is crucial to investigate changes in disease-free animals. Among various hypotheses regarding the effects of acupuncture on the body, we focused on the result that acupuncture stimulation is related to mitochondria.

Objectives: We examined the effects of acupuncture mitochondrial fission and fusionrelated mediators in disease-free Sprague Dawley (SD) rats' spleen meridian acupoints.

Methods: SD rats were divided into control, SP1, SP2, SP3, SP5, and SP9 acupuncture groups. Acupuncture was performed at each point for 10 minutes daily for four days. Peroxisome proliferator-activated receptor-gamma coactivator 1-α (PGC-1α) and fission protein 1 (Fis1) levels were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR), while dynamin-related protein 1 (DRP1), optic atrophy-1 (OPA1), mitofusin-1 (MFN1), and mitofusin-2 (MFN2) levels were assessed via western blotting. Mitochondria protein concentrations and NADH dehydrogenase activity in spleen tissues were measured using enzyme-linked immunosorbent assay (ELISA).

Results: PGC-1α expression decreased in the SP1 (p < 0.01), SP5 (p < 0.05), and SP9 (p < 0.05) groups, while Fis1 expression increased in the SP1 (p < 0.01), SP5 (p < 0.01), and SP9 (p < 0.05) groups. DRP1, OPA1, MFN1, and MFN2 levels exhibited no significant changes. Mitochondrial protein concentrations decreased in the SP2 (p < 0.01), SP3 (p < 0.01), SP5 (p < 0.01), and SP9 (p < 0.01) groups, while NADH dehydrogenase activity decreased in the SP2 (p < 0.05) and SP9 (p < 0.05) groups.

Conclusion: Acupuncture at the SP9 acupoint influenced the mitochondrial fission pathway by modulating PGC-1α and Fis1 mediators in the rat spleen under non-disease conditions.

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来源期刊
Journal of Acupuncture and Meridian Studies
Journal of Acupuncture and Meridian Studies INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
1.50
自引率
10.00%
发文量
35
审稿时长
12 weeks
期刊介绍: The Journal of Acupuncture and Meridian Studies is a quarterly, peer-reviewed journal featuring high-quality studies related to basic and clinical acupuncture and meridian research. It also includes new paradigm of integrative research, covering East–West and traditional–modern medicine. Manuscripts should fall into one of the categories: topical review, original research paper, brief ... click here for full Aims & Scope The Journal of Acupuncture and Meridian Studies is a quarterly, peer-reviewed journal featuring high-quality studies related to basic and clinical acupuncture and meridian research. It also includes new paradigm of integrative research, covering East–West and traditional–modern medicine.
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