基于聚乳酸的三维纳米纤维结构,具有精心设计的形态和增强的比表面积,可用于组织工程。

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Fatemeh Hejazi, Hamid Mirzadeh, Shahrokh Shojaei
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引用次数: 1

摘要

组织工程为生物组织替代应用开辟了新的前景。支架、合适的细胞和信号诱导是任何组织工程应用中主要的三个决定参数。设计一种合适的支架来模拟细胞固有的和自然的居住环境,对细胞的生长和增殖具有重要意义。就像一个天然的细胞外基质(ECM)一样,支架为细胞提供了一个执行生物功能的环境。因此,巨大的表面积和三维纳米纤维结构是组织工程中功能支架的关键特征之一,增强其性能是本研究的主要目的。在我们之前的研究中,引入了一种由连续纳米纤维和微粒子组成的图案结构。在这项工作中,应用了一种新的改性方法来调节静电纺丝/静电喷涂支架的表面积。为此目的,在静电纺丝/静电喷涂期间的预定阶段,氮气以与射流运动相反的方向通过收集器的网孔被冲洗。这种方法通过提供扫氮的冷却效果,导致在氮气冲洗间隔形成非常薄的纳米纤维层。因此,形成了具有极高表面积/体积比的分层结构。对制备的支架进行了孔隙率、吸水率和形态分析。体外细胞相容性评估和组织学分析表明,制备的支架为细胞附着、增殖和浸润提供了合适的底物。这些发现对三维组织工程如骨组织工程的应用具有一定的优势。此外,由于制备的样品具有先进的微观结构和巨大的表面积,它们可以应用于许多其他应用,如膜、过滤等。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PCL-based 3D nanofibrous structure with well-designed morphology and enhanced specific surface area for tissue engineering application.

Tissue engineering opens a new horizon for biological tissue replacement applications. Scaffolds, appropriate cells, and signaling induction are the main three determinant parameters in any tissue engineering applications. Designing a suitable scaffold which can mimic the cellular inherent and natural habitation is of great importance for cellular growth and proliferation. Just like a natural extracellular matrix (ECM), scaffolds provide the cells with an environment for performing biological functions. Accordingly, vast surface area and three-dimensional nanofibrous structures are among the pivotal characteristics of functional scaffolds in tissue engineering, and enhancement of their properties is the main purpose of the present research. In our previous study, a patterned structure composed of continuous nanofibers and microparticles was introduced. In this work, a new modification is applied for adjustment of the surface area of an electrospun/electrosprayed scaffold. For this purpose, at predetermined stages during electrospinning/electrospraying, the nitrogen gas is flushed through the mesh holes of the collector in the opposite direction of the jet movement. This method has led to the formation of very thin nanofibrous layers at nitrogen flush intervals by providing a cooling effect of the sweeping nitrogen. As a consequence, a straticulated structure has been fabricated which possesses extremely high surface/volume ratio. The porosity, water absorption, and morphological analysis were conducted on the obtained scaffold. In vitro cytocompatibility assessments as well as histological analysis demonstrated that the fabricated scaffold provides a proper substrate for cellular attachment, proliferation and infiltration. These findings can be advantageous in three-dimensional tissue engineering such as bone tissue engineering applications. Furthermore, according to the advanced microstructure and vast surface area of the fabricated samples, they can be applied in many other applications, such as membrane, filtration, etc.

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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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