Hakkı Can Ölke, Ömer Sunkar Biçer, Akif Mirioğlu, Dilek Şaker, Işıl Öcal, Cenk Özkan
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A combination of coenzyme Q10 and vitamin E was applied to the repair site by a catheter placed subcutaneously in the treatment group. Only transection-repair was done in the control group. All groups were divided into 2 subgroups for histomorphological, clinical, and electrophysiological experiments because of concerns about possible interference with histomorphological preparation (5 rats in each group). The experiment results were examined by the thermal plantar test, action potential and latency time measurements, and electron microscopy at the end of 4 and 8 weeks. The intact group was studied as the uninterrupted 10 left sciatic nerves of control for 4 weeks.</p><p><strong>Results: </strong>The mean thermal plantar test results of the intact group were better than those of the control groups (P < .05). However, there was no significant difference between the intact and treatment groups. 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引用次数: 1
摘要
目的:探讨局部使用辅酶Q10和维生素E联合对周围神经损伤大鼠模型的临床、电生理和组织形态学的影响。方法:将体重250 ~ 350 g的成年雌性Wistar-Albino大鼠40只，置于温度20 ~ 22℃，明暗循环12小时的室内。他们可以免费获得食物和水。对40只大鼠右侧坐骨神经进行横切修复。将受试者分为4组:对照组(对照组4周和对照组8周)和治疗组(治疗4周和治疗8周)。治疗组通过皮下导管将辅酶Q10和维生素E联合应用于修复部位。对照组仅行横断修复。考虑到可能干扰组织形态学准备，将各组分为2个亚组(每组5只)进行组织形态学、临床和电生理实验。实验4周末和8周末分别用足底热测试、动作电位和潜伏期测量、电镜观察观察实验结果。完整组作为不间断的左侧坐骨神经对照组研究4周。结果:完整组平均足底热测试结果优于对照组(P < 0.05)。然而，在完整组和治疗组之间没有显著差异。在组织形态学检查中，有髓鞘轴突数量明显增加，髓鞘结构更接近于完整组，特别是治疗-8组与对照组比较(control-4: P < 0.0001, control-8: P < 0.01)。结论:局部应用辅酶Q10和维生素E对实验性大鼠坐骨神经横断修复模型有效。
Clinical, electrophysiological, and histomorphological effects of local coenzyme Q10 and vitamin E use in a rat model of peripheral nerve injury.
Objective: This study aimed to investigate the clinical, electrophysiological, and histomorphological effects of local use of coenzyme Q10 and vitamin E combination in a rat model of peripheral nerve injury.
Methods: Forty adult female Wistar-Albino rats weighing 250-350 g were kept in a room with a temperature of 20-22°C and a light/dark cycle of 12 hours. They had free access to food and water. The right sciatic nerves of 40 rats were transected and repaired. Subjects were divided into 4 groups: controls (control-4 weeks and control-8 weeks) and treatments (treatment-4 weeks and treatment-8 weeks). A combination of coenzyme Q10 and vitamin E was applied to the repair site by a catheter placed subcutaneously in the treatment group. Only transection-repair was done in the control group. All groups were divided into 2 subgroups for histomorphological, clinical, and electrophysiological experiments because of concerns about possible interference with histomorphological preparation (5 rats in each group). The experiment results were examined by the thermal plantar test, action potential and latency time measurements, and electron microscopy at the end of 4 and 8 weeks. The intact group was studied as the uninterrupted 10 left sciatic nerves of control for 4 weeks.
Results: The mean thermal plantar test results of the intact group were better than those of the control groups (P < .05). However, there was no significant difference between the intact and treatment groups. In the histomorphological examination, the number of myelinated axons increased significantly, and the myelin structure was closer to that of the intact group, especially when the treatment-8 group was compared with the control groups (control-4: P < .0001, control-8: P < .01).
Conclusion: Local use of coenzyme Q10 and vitamin E seems useful in the experimental rat sciatic nerve transection-repair model.
期刊介绍：
Acta Orthopaedica et Traumatologica Turcica (AOTT) is an international, scientific, open access periodical published in accordance with independent, unbiased, and double-blinded peer-review principles. The journal is the official publication of the Turkish Association of Orthopaedics and Traumatology, and Turkish Society of Orthopaedics and Traumatology. It is published bimonthly in January, March, May, July, September, and November. The publication language of the journal is English.
The aim of the journal is to publish original studies of the highest scientific and clinical value in orthopedics, traumatology, and related disciplines. The scope of the journal includes but not limited to diagnostic, treatment, and prevention methods related to orthopedics and traumatology. Acta Orthopaedica et Traumatologica Turcica publishes clinical and basic research articles, case reports, personal clinical and technical notes, systematic reviews and meta-analyses and letters to the Editor. Proceedings of scientific meetings are also considered for publication.
The target audience of the journal includes healthcare professionals, physicians, and researchers who are interested or working in orthopedics and traumatology field, and related disciplines.
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