{"title":"柬埔寨乙型脑炎病毒媒介种的系统发育和空间分布。","authors":"Rutaiwan Dusadeepong, Pierre-Olivier Maquart, Mallorie Hide, Sebastien Boyer","doi":"10.1111/mve.12678","DOIUrl":null,"url":null,"abstract":"<p>In Southeast Asia, despite the use of Japanese encephalitis vaccines and vaccination coverage, Japanese encephalitis (JE) transmission is still a major public health issue. The main vectors of this virus are mosquitoes from the genus <i>Culex,</i> which diversity and density are important in Southeast Asia. The main vector species of Japanese encephalitis virus (JEV) in Cambodia belong to the Vishnui subgroup. However, their morphological identification solely based on the adult stage remains challenging, making their segregation and detection difficult.</p><p>In order to identify and describe the distribution of the three main JEV vector species in Cambodia, namely <i>Culex vishnui</i>, <i>Cx. pseudovishnui</i> and <i>Cx. tritaeniorhynchus</i>, mosquito samplings were carried out throughout the country in different environments. Phylogenetic analysis of the cytochrome c oxidase subunit I (coI) gene using maximum-likelihood tree with ultrafast bootstrap and phylogeographic analysis were performed. The three main <i>Culex</i> species are phylogenetically separated, and represent two distinct clades, one with <i>Cx. tritaeniorhynchus</i> and the second with <i>Cx. vishnui</i> and <i>Cx. pseudovishnui</i>, the latter appearing as a subgroup of <i>Cx. vishnui</i>. The phylogeographic analysis shows a distribution of the Vishnui subgroup on the entire Cambodian territory with an overlapped distribution areas leading to a sympatric distribution of these species. The three JEV vector species are geographically well-defined with a strong presence of <i>Cx. pseudovishnui</i> in the forest. Combined with the presence of <i>Cx. tritaeniorhynchus</i> and C<i>x. vishnui</i> in rural, peri-urban, and urban areas, the presence of JEV-competent vectors is widespread in Cambodia.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phylogeny and spatial distribution of Japanese encephalitis virus vector species in Cambodia\",\"authors\":\"Rutaiwan Dusadeepong, Pierre-Olivier Maquart, Mallorie Hide, Sebastien Boyer\",\"doi\":\"10.1111/mve.12678\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>In Southeast Asia, despite the use of Japanese encephalitis vaccines and vaccination coverage, Japanese encephalitis (JE) transmission is still a major public health issue. The main vectors of this virus are mosquitoes from the genus <i>Culex,</i> which diversity and density are important in Southeast Asia. The main vector species of Japanese encephalitis virus (JEV) in Cambodia belong to the Vishnui subgroup. However, their morphological identification solely based on the adult stage remains challenging, making their segregation and detection difficult.</p><p>In order to identify and describe the distribution of the three main JEV vector species in Cambodia, namely <i>Culex vishnui</i>, <i>Cx. pseudovishnui</i> and <i>Cx. tritaeniorhynchus</i>, mosquito samplings were carried out throughout the country in different environments. Phylogenetic analysis of the cytochrome c oxidase subunit I (coI) gene using maximum-likelihood tree with ultrafast bootstrap and phylogeographic analysis were performed. The three main <i>Culex</i> species are phylogenetically separated, and represent two distinct clades, one with <i>Cx. tritaeniorhynchus</i> and the second with <i>Cx. vishnui</i> and <i>Cx. pseudovishnui</i>, the latter appearing as a subgroup of <i>Cx. vishnui</i>. The phylogeographic analysis shows a distribution of the Vishnui subgroup on the entire Cambodian territory with an overlapped distribution areas leading to a sympatric distribution of these species. The three JEV vector species are geographically well-defined with a strong presence of <i>Cx. pseudovishnui</i> in the forest. Combined with the presence of <i>Cx. tritaeniorhynchus</i> and C<i>x. vishnui</i> in rural, peri-urban, and urban areas, the presence of JEV-competent vectors is widespread in Cambodia.</p>\",\"PeriodicalId\":18350,\"journal\":{\"name\":\"Medical and Veterinary Entomology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2023-07-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Medical and Veterinary Entomology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/mve.12678\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical and Veterinary Entomology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/mve.12678","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Phylogeny and spatial distribution of Japanese encephalitis virus vector species in Cambodia
In Southeast Asia, despite the use of Japanese encephalitis vaccines and vaccination coverage, Japanese encephalitis (JE) transmission is still a major public health issue. The main vectors of this virus are mosquitoes from the genus Culex, which diversity and density are important in Southeast Asia. The main vector species of Japanese encephalitis virus (JEV) in Cambodia belong to the Vishnui subgroup. However, their morphological identification solely based on the adult stage remains challenging, making their segregation and detection difficult.
In order to identify and describe the distribution of the three main JEV vector species in Cambodia, namely Culex vishnui, Cx. pseudovishnui and Cx. tritaeniorhynchus, mosquito samplings were carried out throughout the country in different environments. Phylogenetic analysis of the cytochrome c oxidase subunit I (coI) gene using maximum-likelihood tree with ultrafast bootstrap and phylogeographic analysis were performed. The three main Culex species are phylogenetically separated, and represent two distinct clades, one with Cx. tritaeniorhynchus and the second with Cx. vishnui and Cx. pseudovishnui, the latter appearing as a subgroup of Cx. vishnui. The phylogeographic analysis shows a distribution of the Vishnui subgroup on the entire Cambodian territory with an overlapped distribution areas leading to a sympatric distribution of these species. The three JEV vector species are geographically well-defined with a strong presence of Cx. pseudovishnui in the forest. Combined with the presence of Cx. tritaeniorhynchus and Cx. vishnui in rural, peri-urban, and urban areas, the presence of JEV-competent vectors is widespread in Cambodia.
期刊介绍:
Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of:
-epidemiology and transmission of vector-borne pathogens
changes in vector distribution that have impact on the pathogen transmission-
arthropod behaviour and ecology-
novel, field evaluated, approaches to biological and chemical control methods-
host arthropod interactions.
Please note that we do not consider submissions in forensic entomology.