在法医尸检收集的尸体尿液和胃内容物中检测利地安非他明及其代谢物d-安非他明。

IF 2.8 4区 医学 Q2 TOXICOLOGY
Suguru Torimitsu, Kanju Saka, Kanako Noritake, Akira Namera, Yohsuke Makino, Rutsuko Yamaguchi, Hirotaro Iwase
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引用次数: 0

摘要

目的:利地安非他明(LDX)是一种由左旋赖氨酸与右苯丙胺(d-安非他明)结合而成的药物,用于治疗注意力缺陷/多动障碍和嗜睡症。在这篇文章中,我们报告了一个法医尸检的情况下,处方药物是未知的尸检。在检测到安非他明的同时,液相色谱-串联质谱(LC-MS/MS)在所有样品中均未检测到甲基苯丙胺。因此,我们的目的是量化尸检样本中LDX的浓度,并证明在这种情况下检测到的安非他明是由于代谢的LDX。方法:尸体解剖时取股静脉血、心脏全血、尿液、胃内容物进行毒理学分析。采用LC-MS/MS进行定性和定量分析。此外,还对检测到的安非他明进行了光学异构体分离。研究了三种不同温度下LDX在全血和尿中的稳定性。结论:本病例报告的结果可能有助于解释尸体中检测到的安非他明是否是LDX的代谢物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Detection of lisdexamfetamine and its metabolite d-amphetamine in urine and gastric contents collected from a cadaver at forensic autopsy.

Detection of lisdexamfetamine and its metabolite d-amphetamine in urine and gastric contents collected from a cadaver at forensic autopsy.

Detection of lisdexamfetamine and its metabolite d-amphetamine in urine and gastric contents collected from a cadaver at forensic autopsy.

Detection of lisdexamfetamine and its metabolite d-amphetamine in urine and gastric contents collected from a cadaver at forensic autopsy.

Purpose: Lisdexamfetamine (LDX), which is used for the treatment of attention-deficit/hyperactivity disorder and narcolepsy, is composed of L-lysine attached to dextroamphetamine (d-amphetamine). In this article, we report a forensic autopsy case in which prescription drugs were unknown at autopsy. While amphetamine was detected, methamphetamine could not be detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in any of samples collected. Thus, we aimed to quantify LDX concentrations in autopsy samples and to prove that the amphetamine detected in this case was due to metabolized LDX.

Methods: Femoral vein blood, cardiac whole blood, urine, and gastric content samples were taken at autopsy for toxicological analysis. Qualitative and quantitative analyses were performed using LC-MS/MS. In addition, optical isomer separation for the amphetamine detected was conducted. The stability of LDX in whole blood and urine was also examined at three different temperatures.

Results: The concentrations of LDX were < 4.00, 30.9, and 4.42 ng/mL in whole blood, urine, and gastric content samples, respectively. The concentrations of amphetamine were 329, 510, 2970, and 915 ng/mL in femoral vein blood, heart whole blood, urine, and gastric contents, respectively. The amphetamine detected in this case was identified to be only d-amphetamine by optical isomer separation. The d-amphetamine detected was considered to be derived from LDX. Stability experiments revealed that LDX in whole blood decreased at ambient temperature.

Conclusions: The results in the present case report may be useful in interpreting whether or not the amphetamine detected in a cadaver is a metabolite of LDX.

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来源期刊
Forensic Toxicology
Forensic Toxicology TOXICOLOGY-
CiteScore
5.80
自引率
9.10%
发文量
40
审稿时长
3 months
期刊介绍: The journal Forensic Toxicology provides an international forum for publication of studies on toxic substances, drugs of abuse, doping agents, chemical warfare agents, and their metabolisms and analyses, which are related to laws and ethics. It includes original articles, reviews, mini-reviews, short communications, and case reports. Although a major focus of the journal is on the development or improvement of analytical methods for the above-mentioned chemicals in human matrices, appropriate studies with animal experiments are also published. Forensic Toxicology is the official publication of the Japanese Association of Forensic Toxicology (JAFT) and is the continuation of the Japanese Journal of Forensic Toxicology (ISSN 0915-9606).
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