Selim Demir , Ilke Onur Kazaz , Gokcen Kerimoglu , Elif Ayazoglu Demir , Fatih Colak , Ayse Firuze Biyik , Yasin Cansever , Ahmet Mentese
{"title":"蜂胶通过减少氧化应激改善缺血/再灌注引起的睾丸损伤","authors":"Selim Demir , Ilke Onur Kazaz , Gokcen Kerimoglu , Elif Ayazoglu Demir , Fatih Colak , Ayse Firuze Biyik , Yasin Cansever , Ahmet Mentese","doi":"10.1016/j.androl.2023.100364","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>This study was performed to evaluate the effect of ethanolic extract of Turkish propolis (EEP) on testicular ischemia/reperfusion (I/R) damage in rats in terms of biochemistry and histopathology, for the first time.</p></div><div><h3>Methods</h3><p>A total of 18 male Sprague-Dawley rats were divided into three groups with six rats in each group: control, torsion/detorsion (T/D), and T/D<!--> <!-->+<!--> <!-->EEP (100<!--> <!-->mg/kg). Testicular torsion was performed by 720° rotating the left testicle in a clockwise direction. The duration of ischemia was 4<!--> <!-->h and orchiectomy was performed after 2<!--> <!-->h of detorsion. EEP was applied only once 30<!--> <span>min before detorsion. Tissue malondialdehyde (MDA), total oxidant status (TOS) and total antioxidant status (TAS) levels were determined using colorimetric methods. Oxidative stress index (OSI) was calculated by proportioning tissue TOS and TAS values to each other. Tissue glutathione (GSH) and glutathione peroxidase (GPx) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits. Johnsen's testicle scoring system was used for histological evaluation.</span></p></div><div><h3>Results</h3><p><span><span><span>In the T/D group, it was determined that statistically significant decreasing in TAS, GSH, GPx levels and Johnsen score, and increasing in TOS, </span>OSI and </span>MDA levels (</span><em>p</em> <!--><<!--> <!-->0.05) compared with control group. EEP administration statistically significantly restored this I/R damage (<em>p</em> <!--><<!--> <!-->0.05).</p></div><div><h3>Conclusion</h3><p>This is the first study to show that propolis prevent I/R-induced testicular damage through its antioxidant activity. More comprehensive studies are needed to see the underlying mechanisms.</p></div>","PeriodicalId":49129,"journal":{"name":"Revista Internacional De Andrologia","volume":"21 3","pages":"Article 100364"},"PeriodicalIF":0.8000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Propolis ameliorates ischemia/reperfusion-induced testicular damage by reducing oxidative stress\",\"authors\":\"Selim Demir , Ilke Onur Kazaz , Gokcen Kerimoglu , Elif Ayazoglu Demir , Fatih Colak , Ayse Firuze Biyik , Yasin Cansever , Ahmet Mentese\",\"doi\":\"10.1016/j.androl.2023.100364\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>This study was performed to evaluate the effect of ethanolic extract of Turkish propolis (EEP) on testicular ischemia/reperfusion (I/R) damage in rats in terms of biochemistry and histopathology, for the first time.</p></div><div><h3>Methods</h3><p>A total of 18 male Sprague-Dawley rats were divided into three groups with six rats in each group: control, torsion/detorsion (T/D), and T/D<!--> <!-->+<!--> <!-->EEP (100<!--> <!-->mg/kg). Testicular torsion was performed by 720° rotating the left testicle in a clockwise direction. The duration of ischemia was 4<!--> <!-->h and orchiectomy was performed after 2<!--> <!-->h of detorsion. EEP was applied only once 30<!--> <span>min before detorsion. Tissue malondialdehyde (MDA), total oxidant status (TOS) and total antioxidant status (TAS) levels were determined using colorimetric methods. Oxidative stress index (OSI) was calculated by proportioning tissue TOS and TAS values to each other. Tissue glutathione (GSH) and glutathione peroxidase (GPx) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits. Johnsen's testicle scoring system was used for histological evaluation.</span></p></div><div><h3>Results</h3><p><span><span><span>In the T/D group, it was determined that statistically significant decreasing in TAS, GSH, GPx levels and Johnsen score, and increasing in TOS, </span>OSI and </span>MDA levels (</span><em>p</em> <!--><<!--> <!-->0.05) compared with control group. EEP administration statistically significantly restored this I/R damage (<em>p</em> <!--><<!--> <!-->0.05).</p></div><div><h3>Conclusion</h3><p>This is the first study to show that propolis prevent I/R-induced testicular damage through its antioxidant activity. More comprehensive studies are needed to see the underlying mechanisms.</p></div>\",\"PeriodicalId\":49129,\"journal\":{\"name\":\"Revista Internacional De Andrologia\",\"volume\":\"21 3\",\"pages\":\"Article 100364\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Internacional De Andrologia\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1698031X23000249\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ANDROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Internacional De Andrologia","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1698031X23000249","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ANDROLOGY","Score":null,"Total":0}
Propolis ameliorates ischemia/reperfusion-induced testicular damage by reducing oxidative stress
Purpose
This study was performed to evaluate the effect of ethanolic extract of Turkish propolis (EEP) on testicular ischemia/reperfusion (I/R) damage in rats in terms of biochemistry and histopathology, for the first time.
Methods
A total of 18 male Sprague-Dawley rats were divided into three groups with six rats in each group: control, torsion/detorsion (T/D), and T/D + EEP (100 mg/kg). Testicular torsion was performed by 720° rotating the left testicle in a clockwise direction. The duration of ischemia was 4 h and orchiectomy was performed after 2 h of detorsion. EEP was applied only once 30 min before detorsion. Tissue malondialdehyde (MDA), total oxidant status (TOS) and total antioxidant status (TAS) levels were determined using colorimetric methods. Oxidative stress index (OSI) was calculated by proportioning tissue TOS and TAS values to each other. Tissue glutathione (GSH) and glutathione peroxidase (GPx) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits. Johnsen's testicle scoring system was used for histological evaluation.
Results
In the T/D group, it was determined that statistically significant decreasing in TAS, GSH, GPx levels and Johnsen score, and increasing in TOS, OSI and MDA levels (p < 0.05) compared with control group. EEP administration statistically significantly restored this I/R damage (p < 0.05).
Conclusion
This is the first study to show that propolis prevent I/R-induced testicular damage through its antioxidant activity. More comprehensive studies are needed to see the underlying mechanisms.
期刊介绍：
Revista Internacional de Andrología es la revista oficial de la Asociación Española de Andrología, Medicina Sexual y Reproductiva (ASESA), la Sociedade Portuguesa de Ardrologia, la Sociedad Argentina de Andrología (SAA), la Asociación Iberoamericana de Sociedades de Andrología (ANDRO), y la Federación Española de Sociedades de Sexología.
La revista publicada trimestralmente es revisada por pares y es líder en el la especialidad y en español y portugués. Recientemente también publica artículos en inglés.
El objetivo de la revista es principalmente la promoción del conocimiento y la educación médica continua, con un enfoque especial en el público español y latinoamericano, a través de la publicación de las contribuciones importantes de la investigación en el campo. Todos los miembros de las sociedades antes mencionadas reciben la revista y otros suscriptores individuales e institucionales de España, Portugal y América Latina.
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