Leonardo S Barros, Giovanna C Denucci, Flávia Lb Amoral, Fabiana Mg Franga, Roberta T Basting, Cecilia P Turssi
{"title":"唾液白蛋白降解复合树脂的潜力。","authors":"Leonardo S Barros, Giovanna C Denucci, Flávia Lb Amoral, Fabiana Mg Franga, Roberta T Basting, Cecilia P Turssi","doi":"10.54589/aol.36/1/34","DOIUrl":null,"url":null,"abstract":"<p><p>Albumin is a salivary enzyme capable of cleaving ester linkages and catalyzing degradation of resin-based dental materials. However, the effect of concentration-dependent esterolytic action on composite resins as yet remains unexplored.</p><p><strong>Aim: </strong>The purpose of this study was to evaluate whether artificial saliva formulations with different concentrations of albumin affected the surface roughness, flexural strength and microhardness of a composite resin.</p><p><strong>Materials and method: </strong>Specimens (25x2x2mm) of a nanofilled composite (Filtek Z350XT, 3M/ESPE) were prepared and analyzed for average surface roughness (Ra/pm). The specimens were then allocated to 6 groups (n=30), to be treated with different salivary albumin concentrations: 0, 10, 50, 100, 200, 400 pg/mL. The specimens were stored in their respective artificial saliva groups, half of them for 24 h and the remainder for 180 days (artificial saliva renewed weekly), after which they were submitted to a new Ra reading, and tested for three-point flexural strength (FS, MPa). The specimens stored for 180 days were analyzed for Knoop microhardness (KH, Kg/mm<sup>2</sup>). Data were submitted to two-way ANOVA (Ra and FS) and one-way ANOVA (KH).</p><p><strong>Results: </strong>Although Ra increased (p < 0.001) and FS decreased (p < 0.001) from 24 hours to 180 days of storage, the albumin concentration did not significantly affectRa (p = 0.168), FS (p = 0.477) or KH (p = 0.378).</p><p><strong>Conclusion: </strong>The esterolytic action of albumin did not increase the artificial-saliva-induced hydrolytic degradation of the composite resin.</p>","PeriodicalId":7033,"journal":{"name":"Acta odontologica latinoamericana : AOL","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/59/22/1852-4834-36-1-34.PMC10283366.pdf","citationCount":"0","resultStr":"{\"title\":\"The potential of salivary albumin to degrade composite resin.\",\"authors\":\"Leonardo S Barros, Giovanna C Denucci, Flávia Lb Amoral, Fabiana Mg Franga, Roberta T Basting, Cecilia P Turssi\",\"doi\":\"10.54589/aol.36/1/34\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Albumin is a salivary enzyme capable of cleaving ester linkages and catalyzing degradation of resin-based dental materials. However, the effect of concentration-dependent esterolytic action on composite resins as yet remains unexplored.</p><p><strong>Aim: </strong>The purpose of this study was to evaluate whether artificial saliva formulations with different concentrations of albumin affected the surface roughness, flexural strength and microhardness of a composite resin.</p><p><strong>Materials and method: </strong>Specimens (25x2x2mm) of a nanofilled composite (Filtek Z350XT, 3M/ESPE) were prepared and analyzed for average surface roughness (Ra/pm). The specimens were then allocated to 6 groups (n=30), to be treated with different salivary albumin concentrations: 0, 10, 50, 100, 200, 400 pg/mL. The specimens were stored in their respective artificial saliva groups, half of them for 24 h and the remainder for 180 days (artificial saliva renewed weekly), after which they were submitted to a new Ra reading, and tested for three-point flexural strength (FS, MPa). The specimens stored for 180 days were analyzed for Knoop microhardness (KH, Kg/mm<sup>2</sup>). Data were submitted to two-way ANOVA (Ra and FS) and one-way ANOVA (KH).</p><p><strong>Results: </strong>Although Ra increased (p < 0.001) and FS decreased (p < 0.001) from 24 hours to 180 days of storage, the albumin concentration did not significantly affectRa (p = 0.168), FS (p = 0.477) or KH (p = 0.378).</p><p><strong>Conclusion: </strong>The esterolytic action of albumin did not increase the artificial-saliva-induced hydrolytic degradation of the composite resin.</p>\",\"PeriodicalId\":7033,\"journal\":{\"name\":\"Acta odontologica latinoamericana : AOL\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-04-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/59/22/1852-4834-36-1-34.PMC10283366.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta odontologica latinoamericana : AOL\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.54589/aol.36/1/34\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta odontologica latinoamericana : AOL","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.54589/aol.36/1/34","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The potential of salivary albumin to degrade composite resin.
Albumin is a salivary enzyme capable of cleaving ester linkages and catalyzing degradation of resin-based dental materials. However, the effect of concentration-dependent esterolytic action on composite resins as yet remains unexplored.
Aim: The purpose of this study was to evaluate whether artificial saliva formulations with different concentrations of albumin affected the surface roughness, flexural strength and microhardness of a composite resin.
Materials and method: Specimens (25x2x2mm) of a nanofilled composite (Filtek Z350XT, 3M/ESPE) were prepared and analyzed for average surface roughness (Ra/pm). The specimens were then allocated to 6 groups (n=30), to be treated with different salivary albumin concentrations: 0, 10, 50, 100, 200, 400 pg/mL. The specimens were stored in their respective artificial saliva groups, half of them for 24 h and the remainder for 180 days (artificial saliva renewed weekly), after which they were submitted to a new Ra reading, and tested for three-point flexural strength (FS, MPa). The specimens stored for 180 days were analyzed for Knoop microhardness (KH, Kg/mm2). Data were submitted to two-way ANOVA (Ra and FS) and one-way ANOVA (KH).
Results: Although Ra increased (p < 0.001) and FS decreased (p < 0.001) from 24 hours to 180 days of storage, the albumin concentration did not significantly affectRa (p = 0.168), FS (p = 0.477) or KH (p = 0.378).
Conclusion: The esterolytic action of albumin did not increase the artificial-saliva-induced hydrolytic degradation of the composite resin.