唾液白蛋白降解复合树脂的潜力。

Leonardo S Barros, Giovanna C Denucci, Flávia Lb Amoral, Fabiana Mg Franga, Roberta T Basting, Cecilia P Turssi
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引用次数: 0

摘要

白蛋白是一种唾液酶,能够切断酯键并催化树脂基牙科材料的降解。然而,浓度依赖性的酯水解作用对复合树脂的影响仍未被探索。目的:本研究的目的是评估不同浓度白蛋白的人工唾液配方是否影响复合树脂的表面粗糙度、抗弯强度和显微硬度。材料和方法:制备纳米填充复合材料(Filtek Z350XT, 3M/ESPE)的试样(25x2x2mm),分析其平均表面粗糙度(Ra/pm)。将标本分为6组(n=30),分别给予0、10、50、100、200、400 pg/mL不同浓度的唾液白蛋白处理。将标本分别保存在各自的人工唾液组中,其中一半保存24 h,其余保存180天(每周更新人工唾液),之后提交新的Ra读数,并进行三点抗弯强度(FS, MPa)测试。保存180 d的标本进行Knoop显微硬度(KH, Kg/mm2)分析。数据进行双向方差分析(Ra和FS)和单向方差分析(KH)。结果:贮藏24 ~ 180 d, Ra升高(p < 0.001), FS降低(p < 0.001),但白蛋白浓度对tra (p = 0.168)、FS (p = 0.477)、KH (p = 0.378)无显著影响。结论:白蛋白的酯解作用不增加人工唾液诱导的复合树脂水解降解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The potential of salivary albumin to degrade composite resin.

Albumin is a salivary enzyme capable of cleaving ester linkages and catalyzing degradation of resin-based dental materials. However, the effect of concentration-dependent esterolytic action on composite resins as yet remains unexplored.

Aim: The purpose of this study was to evaluate whether artificial saliva formulations with different concentrations of albumin affected the surface roughness, flexural strength and microhardness of a composite resin.

Materials and method: Specimens (25x2x2mm) of a nanofilled composite (Filtek Z350XT, 3M/ESPE) were prepared and analyzed for average surface roughness (Ra/pm). The specimens were then allocated to 6 groups (n=30), to be treated with different salivary albumin concentrations: 0, 10, 50, 100, 200, 400 pg/mL. The specimens were stored in their respective artificial saliva groups, half of them for 24 h and the remainder for 180 days (artificial saliva renewed weekly), after which they were submitted to a new Ra reading, and tested for three-point flexural strength (FS, MPa). The specimens stored for 180 days were analyzed for Knoop microhardness (KH, Kg/mm2). Data were submitted to two-way ANOVA (Ra and FS) and one-way ANOVA (KH).

Results: Although Ra increased (p < 0.001) and FS decreased (p < 0.001) from 24 hours to 180 days of storage, the albumin concentration did not significantly affectRa (p = 0.168), FS (p = 0.477) or KH (p = 0.378).

Conclusion: The esterolytic action of albumin did not increase the artificial-saliva-induced hydrolytic degradation of the composite resin.

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