没食子酸辛酯作为抗氧化食品添加剂在体外试验系统中的遗传毒性研究。

IF 2.5 4区 医学 Q3 GENETICS & HEREDITY
Mutagenesis Pub Date : 2023-06-20 DOI:10.1093/mutage/gead005
Ece Avuloglu Yilmaz, Deniz Yuzbasioglu, Fatma Unal
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引用次数: 0

摘要

几种抗氧化食品添加剂被添加到油、汤、酱、口香糖、薯片等中。其中之一是没食子酸辛酯。本研究的目的是通过体外染色体异常(CA)、姐妹染色单体交换(SCE)、细胞分裂阻断微核细胞组(CBMN-Cyt)、微核- fish (MN-FISH)和彗星试验来评估没食子酸辛酯对人淋巴细胞的潜在遗传毒性。采用不同浓度(0.031、0.063、0.125、0.25、0.50 μg/ml)的没食子酸辛酯。每个处理分别为阴性(蒸馏水)、阳性(0.20 μl/ml丝裂霉素- c)和溶剂对照(8.77 μl/ml乙醇)。没食子酸辛酯未引起染色体异常、微核、核芽(NBUD)和核质桥(NPB)频率的改变。同样,与溶剂对照相比,DNA损伤(彗星试验),着丝粒阳性和阴性细胞的百分比(MN-FISH试验)没有显著差异。没食子酸辛酯对复制和核分裂指数没有影响。另一方面,在处理24 h时,与溶剂对照相比,在三个最高浓度下,SCE/细胞比显著增加。同样,在处理48 h时,在所有浓度(0.031 μg/ml除外)下,与溶剂对照相比,SCE的频率显著升高。在处理24 h的最高浓度和48 h的几乎所有浓度(0.031和0.063µg/ml除外)中,有丝分裂指数值都有显著降低。结果表明,在本研究中应用的浓度下,没食子酸辛酯对人外周血淋巴细胞没有重要的遗传毒理学作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of genotoxic effect of octyl gallate used as an antioxidant food additive in in vitro test systems.
Abstract Several antioxidant food additives are added to oils, soups, sauces, chewing gum, potato chips, and so on. One of them is octyl gallate. The purpose of this study was to evaluate the potential genotoxicity of octyl gallate in human lymphocytes, using in vitro chromosomal abnormalities (CA), sister chromatid exchange (SCE), cytokinesis block micronucleus cytome (CBMN-Cyt), micronucleus-FISH (MN-FISH), and comet tests. Different concentrations (0.031, 0.063, 0.125, 0.25, and 0.50 μg/ml) of octyl gallate were used. A negative (distilled water), a positive (0.20 μg/ml Mitomycin-C), and a solvent control (8.77 μl/ml ethanol) were also applied for each treatment. Octyl gallate did not cause changes in chromosomal abnormalities, micronucleus, nuclear bud (NBUD), and nucleoplasmic bridge (NPB) frequency. Similarly, there was no significant difference in DNA damage (comet assay), percentage of centromere positive and negative cells (MN-FISH test) compared to the solvent control. Moreover, octyl gallate did not affect replication and nuclear division index. On the other hand, it significantly increased the SCE/cell ratio in three highest concentrations compared to solvent control at 24 h treatment. Similarly, at 48 h treatment, the frequency of SCE raised significantly compared to solvent controls at all the concentrations (except 0.031 μg/ml). An important reduction was detected in mitotic index values in the highest concentration at 24 h treatment and almost all concentrations (except 0.031 and 0.063 µg/ml) at 48 h treatment. The results obtained suggest that octyl gallate has no important genotoxicological action on human peripheral lymphocytes at the concentrations applied in this study.
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来源期刊
Mutagenesis
Mutagenesis 生物-毒理学
CiteScore
5.90
自引率
3.70%
发文量
22
审稿时长
6-12 weeks
期刊介绍: Mutagenesis is an international multi-disciplinary journal designed to bring together research aimed at the identification, characterization and elucidation of the mechanisms of action of physical, chemical and biological agents capable of producing genetic change in living organisms and the study of the consequences of such changes.
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