人类肠道病毒组的群落类型和溃疡性结肠炎的内镜检查结果有关。

IF 8.3 2区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY
Daan Jansen, Gwen Falony, Sara Vieira-Silva, Ceren Simsek, Tine Marcelis, Clara Caenepeel, Kathleen Machiels, Jeroen Raes, Séverine Vermeire, Jelle Matthijnssens
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引用次数: 0

摘要

背景:炎症性肠病是一种主要的使人衰弱的疾病。近年来,肠道微生物群作为参与IBD病理生理学的重要因素而受到关注。作为对已建立的与IBD相关的细菌“肠道类型”的补充,我们在这里重点关注病毒。我们调查了接受生物治疗的IBD患者的肠道病毒组,以确定是否存在与IBD相关的病毒组构型,并揭示这些构型如何与治疗成功相关。方法:对181名开始生物治疗的IBD患者的432份粪便样本进行病毒样颗粒富集,然后进行深度测序。冗余度分析和Dirichlet多项式混合物分别用于确定病毒组组成的协变量,并将肠道病毒群浓缩为“病毒群落类型”。结果:根据无监督聚类将患者分为两种病毒群落类型。群落型CA表现出较低的α-多样性和较高的Caudoviricetes[非CrAss]噬菌体相对丰度,并与失调菌2型肠型有关。群落型CrM表现出较高的α-多样性和较高的Crassvirales和Malgrandaviretes噬菌体的相对丰度。在介入后分析中,内镜检查结果与肠道病毒组分有关。缓解型UC患者具有较高的社区型CrM百分比、较高的Shannon多样性和较低的溶原潜力。介入前分析还发现了五种与治疗成功相关的新型噬菌体。结论:本研究提出了两种可能参与IBD病理生理学的肠道病毒组结构。有趣的是,这些病毒配置与治疗成功进一步相关,这表明了潜在的临床相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Community Types of the Human Gut Virome are Associated with Endoscopic Outcome in Ulcerative Colitis.

Community Types of the Human Gut Virome are Associated with Endoscopic Outcome in Ulcerative Colitis.

Community Types of the Human Gut Virome are Associated with Endoscopic Outcome in Ulcerative Colitis.

Community Types of the Human Gut Virome are Associated with Endoscopic Outcome in Ulcerative Colitis.

Background: Inflammatory bowel disease [IBD] is a major debilitating disease. Recently, the gut microbiota has gained attention as an important factor involved in the pathophysiology of IBD. As a complement to the established bacterial 'enterotypes' associated with IBD, we focused here on viruses. We investigated the intestinal virome of IBD patients undergoing biological therapy for the presence of virome configurations associated with IBD, and to uncover how those configurations are associated with therapeutic success.

Methods: Viral-like particle enrichment followed by deep sequencing was performed on 432 faecal samples from 181 IBD patients starting biological therapy. Redundancy analysis and Dirichlet Multinomial Mixtures were applied to determine covariates of the virome composition and to condense the gut virota into 'viral community types', respectively.

Results: Patients were stratified based on unsupervised clustering into two viral community types. Community type CA showed a low α-diversity and a high relative abundance of Caudoviricetes [non-CrAss] phages and was associated with the dysbiotic Bact2-enterotype. Community type CrM showed a high α-diversity and a high relative abundance of Crassvirales and Malgrandaviricetes phages. During post-interventional analysis, endoscopic outcome was associated with gut virome composition. Remitting UC patients had a high percentage of community type CrM, a high Shannon diversity and a low lysogenic potential. Pre-interventional analyses also identified five novel phages associated with treatment success.

Conclusions: This study proposed two gut virome configurations that may be involved in the pathophysiology of IBD. Interestingly, those viral configurations are further associated with therapeutic success, suggesting a potential clinical relevance.

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来源期刊
Journal of Crohns & Colitis
Journal of Crohns & Colitis 医学-胃肠肝病学
CiteScore
15.50
自引率
7.50%
发文量
1048
审稿时长
1 months
期刊介绍: Journal of Crohns and Colitis is concerned with the dissemination of knowledge on clinical, basic science and innovative methods related to inflammatory bowel diseases. The journal publishes original articles, review papers, editorials, leading articles, viewpoints, case reports, innovative methods and letters to the editor.
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