Gaowa Hasi, Liyasu Wu, Tserennadmid Sodnompil, Ruhan Yi, Rihan Wu, Rui Zhang, Haya Na, Hejie Liu, Musi Ji, Wangwei Xie, Narenhua Nasenochir
{"title":"热休克蛋白70 (HSP70)和谷胱甘肽过氧化物酶5 (GPX5)在双峰驼睾丸和附睾中的差异表达和定位。","authors":"Gaowa Hasi, Liyasu Wu, Tserennadmid Sodnompil, Ruhan Yi, Rihan Wu, Rui Zhang, Haya Na, Hejie Liu, Musi Ji, Wangwei Xie, Narenhua Nasenochir","doi":"10.1071/RD23026","DOIUrl":null,"url":null,"abstract":"<p><strong>Context: </strong>Heat shock protein 70 (HSP70) and glutathione peroxidase 5 (GPX5) are biomarkers of oxidative stress and stress in temperate, tropical environments, which are crucial for male reproduction. Their expression and distribution patterns in the testis and epididymis of Bactrian camels are still unknown.</p><p><strong>Aims: </strong>This study aims to investigate the HSP70 and GPX5 expression and localisation in 3- and 6-year-old Bactrian camel testis and epididymis.</p><p><strong>Methods: </strong>Reverse transcription quantitative polymerase chain reaction (qRT-PCR), Western blot and immunohistochemistry were used to detect HSP70 in the testis and epididymis (caput, corpus and cauda) and GPX5 in the epididymis at two developmental stages (3-year-old puberty group and 6-year-old adult group).</p><p><strong>Key results: </strong>HSP70 was upregulated in the testis. Immunohistochemistry results indicated the HSP70 protein was mainly detected in spermatids and Leydig cells of testicular tissue. In the epididymis, HSP70 was located at the luminal spermatozoa, the epithelium lining the epididymal and the epididymal interstitium. GPX5 expression was significantly higher in the caput epididymis than in the corpus and cauda epididymis. GPX5 protein was observed in the epithelium lining the epididymal, interstitium and luminal spermatozoa in the epididymis by immunohistochemistry.</p><p><strong>Conclusions: </strong>Bactrian camel HSP70 and GPX5 exhibited spatiotemporal expression specificity.</p><p><strong>Implications: </strong>HSP70 and GPX5 may be essential for germ cell development and reproductive success after sexual maturation in Sonid Bactrian camels.</p>","PeriodicalId":20932,"journal":{"name":"Reproduction, fertility, and development","volume":"35 10","pages":"552-562"},"PeriodicalIF":1.8000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Differential expression and localisation of heat shock protein 70 (HSP70) and glutathione peroxidase 5 (GPX5) in the testis and epididymis of Sonid Bactrian camels.\",\"authors\":\"Gaowa Hasi, Liyasu Wu, Tserennadmid Sodnompil, Ruhan Yi, Rihan Wu, Rui Zhang, Haya Na, Hejie Liu, Musi Ji, Wangwei Xie, Narenhua Nasenochir\",\"doi\":\"10.1071/RD23026\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Context: </strong>Heat shock protein 70 (HSP70) and glutathione peroxidase 5 (GPX5) are biomarkers of oxidative stress and stress in temperate, tropical environments, which are crucial for male reproduction. Their expression and distribution patterns in the testis and epididymis of Bactrian camels are still unknown.</p><p><strong>Aims: </strong>This study aims to investigate the HSP70 and GPX5 expression and localisation in 3- and 6-year-old Bactrian camel testis and epididymis.</p><p><strong>Methods: </strong>Reverse transcription quantitative polymerase chain reaction (qRT-PCR), Western blot and immunohistochemistry were used to detect HSP70 in the testis and epididymis (caput, corpus and cauda) and GPX5 in the epididymis at two developmental stages (3-year-old puberty group and 6-year-old adult group).</p><p><strong>Key results: </strong>HSP70 was upregulated in the testis. Immunohistochemistry results indicated the HSP70 protein was mainly detected in spermatids and Leydig cells of testicular tissue. In the epididymis, HSP70 was located at the luminal spermatozoa, the epithelium lining the epididymal and the epididymal interstitium. GPX5 expression was significantly higher in the caput epididymis than in the corpus and cauda epididymis. GPX5 protein was observed in the epithelium lining the epididymal, interstitium and luminal spermatozoa in the epididymis by immunohistochemistry.</p><p><strong>Conclusions: </strong>Bactrian camel HSP70 and GPX5 exhibited spatiotemporal expression specificity.</p><p><strong>Implications: </strong>HSP70 and GPX5 may be essential for germ cell development and reproductive success after sexual maturation in Sonid Bactrian camels.</p>\",\"PeriodicalId\":20932,\"journal\":{\"name\":\"Reproduction, fertility, and development\",\"volume\":\"35 10\",\"pages\":\"552-562\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reproduction, fertility, and development\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1071/RD23026\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproduction, fertility, and development","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1071/RD23026","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
Differential expression and localisation of heat shock protein 70 (HSP70) and glutathione peroxidase 5 (GPX5) in the testis and epididymis of Sonid Bactrian camels.
Context: Heat shock protein 70 (HSP70) and glutathione peroxidase 5 (GPX5) are biomarkers of oxidative stress and stress in temperate, tropical environments, which are crucial for male reproduction. Their expression and distribution patterns in the testis and epididymis of Bactrian camels are still unknown.
Aims: This study aims to investigate the HSP70 and GPX5 expression and localisation in 3- and 6-year-old Bactrian camel testis and epididymis.
Methods: Reverse transcription quantitative polymerase chain reaction (qRT-PCR), Western blot and immunohistochemistry were used to detect HSP70 in the testis and epididymis (caput, corpus and cauda) and GPX5 in the epididymis at two developmental stages (3-year-old puberty group and 6-year-old adult group).
Key results: HSP70 was upregulated in the testis. Immunohistochemistry results indicated the HSP70 protein was mainly detected in spermatids and Leydig cells of testicular tissue. In the epididymis, HSP70 was located at the luminal spermatozoa, the epithelium lining the epididymal and the epididymal interstitium. GPX5 expression was significantly higher in the caput epididymis than in the corpus and cauda epididymis. GPX5 protein was observed in the epithelium lining the epididymal, interstitium and luminal spermatozoa in the epididymis by immunohistochemistry.
Conclusions: Bactrian camel HSP70 and GPX5 exhibited spatiotemporal expression specificity.
Implications: HSP70 and GPX5 may be essential for germ cell development and reproductive success after sexual maturation in Sonid Bactrian camels.
期刊介绍：
Reproduction, Fertility and Development is an international journal for the publication of original and significant contributions on vertebrate reproductive and developmental biology. Subject areas include, but are not limited to: physiology, biochemistry, cell and molecular biology, endocrinology, genetics and epigenetics, behaviour, immunology and the development of reproductive technologies in humans, livestock and wildlife, and in pest management.
Reproduction, Fertility and Development is a valuable resource for research scientists working in industry or academia on reproductive and developmental biology, clinicians and veterinarians interested in the basic science underlying their disciplines, and students.
Reproduction, Fertility and Development is the official journal of the International Embryo Technology Society and the Society for Reproductive Biology.
Reproduction, Fertility and Development is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.
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