六种生化分析物黄疸干扰指数的定义。

IF 3.8 3区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Ruth Cano-Corres, Gemma Sole-Enrech, Maria Isabel Aparicio-Calvente
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引用次数: 0

摘要

黄疸,如果没有检测到,会影响临床实验室提供的结果的有效性,导致错误的结果。本研究旨在定义某些生化分析物的胆红素干扰,并将其与制造商的数据进行比较。材料和方法:用门诊患者的样品制备血清池,加入增加胆红素浓度(默克,参考14370,达姆施塔特,德国)至513µmol/L,以评估以下生化分析物的偏倚:肌酐(CREA),肌酸激酶(CK),胆固醇(CHOL), γ -谷氨酰转移酶(GGT),高密度脂蛋白胆固醇(HDL)和总蛋白(TP)。对于每种分析物,准备了六个不同浓度的池。测量采用Cobas 8000分析仪c702-502,罗氏诊断公司(曼海姆,德国)。本研究采用西班牙检验医学学会定义的研究程序。结果:对CHOL产生负干扰的胆红素浓度为103 μ mol/L, TP为205 μ mol/L, CK为410 μ mol/L,但仅当CK值小于100 U/L时才会产生负干扰。低于513µmol/L的胆红素浓度不会对HDL和GGT产生干扰。最后,对于所研究的胆红素浓度,CREA高于80µmol/L时没有干扰。结论:每种分析物都定义了黄疸干扰,观察到与制造商提供的数据相比的差异。证据表明,每个实验室都应该评估黄疸干扰,以确保交付结果的高质量,从而有利于患者护理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Definition of icteric interference index for six biochemical analytes.

Definition of icteric interference index for six biochemical analytes.

Introduction: Icterus, if not detected, can affect the validity of results delivered by clinical laboratories, leading to erroneous results. This study aims to define bilirubin interference for some biochemical analytes and compare it with the manufacturer's data.

Material and methods: Serum pools prepared with outpatients' samples were spiked with increasing bilirubin concentration (Merck, reference14370, Darmstadt, Germany) up to 513 µmol/L in order to evaluate the bias for the following biochemical analytes: creatinine (CREA), creatine kinase (CK), cholesterol (CHOL), gamma-glutamyltransferase (GGT), high-density lipoprotein cholesterol (HDL), and total protein (TP). For each analyte, six pools of different concentrations were prepared. Measurements were made employing Cobas 8000 analyser c702-502, Roche Diagnostics (Mannheim, Germany). This study employed a study procedure defined by the Spanish Society of Laboratory Medicine.

Results: Obtained bilirubin concentrations producing a negative interference were 103 µmol/L for CHOL, 205 µmol/L for TP and 410 µmol/L for CK, but only for CK values less than 100 U/L. Bilirubin concentrations lower than 513 µmol/L do not produce interference for HDL and GGT. Finally, for the studied bilirubin concentrations, there is no interference for CREA higher than 80 µmol/L.

Conclusion: Icterus interferences have been defined for each analyte, observing differences compared to data provided by the manufacturer. The evidence indicates that each laboratory should evaluate icteric interferences to ensure the high quality of the delivered results, thus benefiting patient care.

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来源期刊
Biochemia Medica
Biochemia Medica 医学-医学实验技术
CiteScore
5.50
自引率
3.00%
发文量
70
审稿时长
>12 weeks
期刊介绍: Biochemia Medica is the official peer-reviewed journal of the Croatian Society of Medical Biochemistry and Laboratory Medicine. Journal provides a wide coverage of research in all aspects of clinical chemistry and laboratory medicine. Following categories fit into the scope of the Journal: general clinical chemistry, haematology and haemostasis, molecular diagnostics and endocrinology. Development, validation and verification of analytical techniques and methods applicable to clinical chemistry and laboratory medicine are welcome as well as studies dealing with laboratory organization, automation and quality control. Journal publishes on a regular basis educative preanalytical case reports (Preanalytical mysteries), articles dealing with applied biostatistics (Lessons in biostatistics) and research integrity (Research integrity corner).
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