{"title":"尿脑源性神经营养因子和神经生长因子作为儿童膀胱过度活动的无创生物标志物。","authors":"Merima Colic, Dunja Rogic, Jasna Lenicek Krleza, Ana Kozmar, Lorna Stemberger Maric, Slaven Abdovic","doi":"10.11613/BM.2022.030706","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Overactive bladder (OAB) is the most common urinary disorder and the leading cause of functional daytime intermittent urinary incontinence in children. The aim of this study was to determine whether urinary brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) concentrations, normalized to urine creatinine, could be used as biomarkers for diagnosis and treatment monitoring of OAB in children.</p><p><strong>Materials and methods: </strong>Urine samples of 48 pediatric patients with OAB were collected at the start of anticholinergic therapy (baseline), at follow-up visits (3 and 6 months), and from 48 healthy controls. Urinary BDNF and NGF concentrations were determined by ELISA method (Merck, Darmstadt, Germany) and Luminex method (Thermo Fisher Scientific, Waltham, USA). Differences of frequency between quantifiable analyte concentrations between subject groups were determined using Fisher's exact test.</p><p><strong>Results: </strong>There was no statistically significant difference between quantifiable analyte concentrations between patients at baseline and the control group for BDNF and NGF by either the ELISA or Luminex method (P = 1.000, P = 0.170, P = 1.000, and P = N/A, respectively). There was a statistically significant difference between quantifiable BDNF by the ELISA method between patients at baseline and complete success follow-up (P = 0.027), while BDNF by Luminex method and NGF by both methods were not statistically significant (P = 0.078, P = 0.519, and P = N/A, respectively).</p><p><strong>Conclusions: </strong>This study did not demonstrate that urinary BDNF and NGF concentrations, can be used as biomarkers for diagnosis and therapy monitoring of OAB in children.</p>","PeriodicalId":9021,"journal":{"name":"Biochemia Medica","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562800/pdf/","citationCount":"0","resultStr":"{\"title\":\"Urinary brain-derived neurotrophic factor and nerve growth factor as noninvasive biomarkers of overactive bladder in children.\",\"authors\":\"Merima Colic, Dunja Rogic, Jasna Lenicek Krleza, Ana Kozmar, Lorna Stemberger Maric, Slaven Abdovic\",\"doi\":\"10.11613/BM.2022.030706\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Overactive bladder (OAB) is the most common urinary disorder and the leading cause of functional daytime intermittent urinary incontinence in children. 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Differences of frequency between quantifiable analyte concentrations between subject groups were determined using Fisher's exact test.</p><p><strong>Results: </strong>There was no statistically significant difference between quantifiable analyte concentrations between patients at baseline and the control group for BDNF and NGF by either the ELISA or Luminex method (P = 1.000, P = 0.170, P = 1.000, and P = N/A, respectively). 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引用次数: 0
摘要
膀胱过动症(OAB)是最常见的泌尿系统疾病,也是儿童日间功能性间歇性尿失禁的主要原因。本研究的目的是确定尿脑源性神经营养因子(BDNF)和神经生长因子(NGF)浓度与尿肌酐归一化是否可以作为儿童OAB诊断和治疗监测的生物标志物。材料和方法:收集48例OAB患儿在抗胆碱能治疗开始(基线)、随访(3个月和6个月)和48例健康对照者的尿液样本。采用ELISA法(Merck, Darmstadt, Germany)和Luminex法(Thermo Fisher Scientific, Waltham, USA)测定尿液BDNF和NGF浓度。使用Fisher精确检验确定受试者组间可量化分析物浓度之间的频率差异。结果:ELISA法和Luminex法测定基线时患者BDNF和NGF可量化分析物浓度与对照组比较,差异均无统计学意义(P = 1.000, P = 0.170, P = 1.000, P = N/A)。基线时和完全成功随访时,ELISA法可量化BDNF差异有统计学意义(P = 0.027),而Luminex法BDNF和两种方法的NGF均无统计学意义(P = 0.078, P = 0.519, P = N/ a)。结论:本研究并未证明尿BDNF和NGF浓度可作为儿童OAB诊断和治疗监测的生物标志物。
Urinary brain-derived neurotrophic factor and nerve growth factor as noninvasive biomarkers of overactive bladder in children.
Introduction: Overactive bladder (OAB) is the most common urinary disorder and the leading cause of functional daytime intermittent urinary incontinence in children. The aim of this study was to determine whether urinary brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) concentrations, normalized to urine creatinine, could be used as biomarkers for diagnosis and treatment monitoring of OAB in children.
Materials and methods: Urine samples of 48 pediatric patients with OAB were collected at the start of anticholinergic therapy (baseline), at follow-up visits (3 and 6 months), and from 48 healthy controls. Urinary BDNF and NGF concentrations were determined by ELISA method (Merck, Darmstadt, Germany) and Luminex method (Thermo Fisher Scientific, Waltham, USA). Differences of frequency between quantifiable analyte concentrations between subject groups were determined using Fisher's exact test.
Results: There was no statistically significant difference between quantifiable analyte concentrations between patients at baseline and the control group for BDNF and NGF by either the ELISA or Luminex method (P = 1.000, P = 0.170, P = 1.000, and P = N/A, respectively). There was a statistically significant difference between quantifiable BDNF by the ELISA method between patients at baseline and complete success follow-up (P = 0.027), while BDNF by Luminex method and NGF by both methods were not statistically significant (P = 0.078, P = 0.519, and P = N/A, respectively).
Conclusions: This study did not demonstrate that urinary BDNF and NGF concentrations, can be used as biomarkers for diagnosis and therapy monitoring of OAB in children.
期刊介绍:
Biochemia Medica is the official peer-reviewed journal of the Croatian Society of Medical Biochemistry and Laboratory Medicine. Journal provides a wide coverage of research in all aspects of clinical chemistry and laboratory medicine. Following categories fit into the scope of the Journal: general clinical chemistry, haematology and haemostasis, molecular diagnostics and endocrinology. Development, validation and verification of analytical techniques and methods applicable to clinical chemistry and laboratory medicine are welcome as well as studies dealing with laboratory organization, automation and quality control. Journal publishes on a regular basis educative preanalytical case reports (Preanalytical mysteries), articles dealing with applied biostatistics (Lessons in biostatistics) and research integrity (Research integrity corner).